Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   D5S10_RS02040 Genome accession   NZ_CP032456
Coordinates   447523..448587 (+) Length   354 a.a.
NCBI ID   WP_002551652.1    Uniprot ID   A0A7Z6Y2Y4
Organism   Pseudomonas savastanoi strain CAS03     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 442523..453587
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D5S10_RS02030 (D5S10_02235) - 442684..443952 (+) 1269 WP_002551650.1 malic enzyme-like NAD(P)-binding protein -
  D5S10_RS02035 (D5S10_02265) - 444871..447313 (-) 2443 Protein_409 penicillin-binding protein 1A -
  D5S10_RS02040 (D5S10_02270) pilM 447523..448587 (+) 1065 WP_002551652.1 pilus assembly protein PilM Machinery gene
  D5S10_RS02045 (D5S10_02275) - 448587..449186 (+) 600 WP_004654254.1 PilN domain-containing protein -
  D5S10_RS02050 (D5S10_02280) pilO 449183..449806 (+) 624 WP_002551654.1 type 4a pilus biogenesis protein PilO -
  D5S10_RS02055 (D5S10_02285) pilP 449803..450330 (+) 528 WP_002551655.1 type 4a pilus biogenesis lipoprotein PilP -
  D5S10_RS02060 (D5S10_02290) pilQ 450378..452483 (+) 2106 WP_005743117.1 type IV pilus secretin PilQ Machinery gene
  D5S10_RS02065 (D5S10_02295) aroK 452488..453006 (+) 519 WP_002551657.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38078.57 Da        Isoelectric Point: 4.7008

>NTDB_id=315983 D5S10_RS02040 WP_002551652.1 447523..448587(+) (pilM) [Pseudomonas savastanoi strain CAS03]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLAKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=315983 D5S10_RS02040 WP_002551652.1 447523..448587(+) (pilM) [Pseudomonas savastanoi strain CAS03]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACTCGTTACAAGGTCGAGTCTTACGCAGTCGAGCCGTTGCCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCCGAGCTCGAAGGGGTTGGTCAGGCGCTGTCTCGCGTGCTCGCCAAGGCCAAGACCAGCGTCAAGATCGTGGCGGTC
GCCGTAGCCGGTTCCGCCGTGATCACCAAGACGATTGAAATGGACGCCGGTCTTTCCGACGACGACATGGAAAACCAGCT
CAAGCTTGAGGCTGACCAGTACATCCCTTATCCGCTGGAAGAAGTCGCCATCGATTTCGAAGTGCAGGGCTACTCGGTGC
GCAATCCCGAACGCGTCGAAGTATTGCTGGCTGCCTGCCGCAAAGAGAACGTCGAAGTGCGTGAGGCGGCGCTGGCTCTG
GCCGGGCTGACTGCGCGCGTGGTGGACGTCGAGGCCTATGCCCTGGAGCGCTCGTTCGGCCTGCTGGCTGCGCAACTGGG
TAACGGTCATGACGAGCTTACCGTGGCGGTGGTCGATATCGGTGCCACCATGACCACCCTCAGCGTGCTGCATCATGGCC
GGATCATCTATACCCGCGAGCAACTGTTTGGTGGCCGCCAGCTGACTGACGAAATCCAGCGCCGCTATGGGCTTTCCATG
GAGGAGGCCGGGCTTGCCAAGAAGCAGGGCGGGTTGCCCGACGACTATGTCAGCGAGGTGCTGGACCCCTTCAAGGATGC
GTTGGTGCAGCAGGTCTCCCGTTCTTTGCAGTTCTTCTTTGCGGCCGGTCAATACAACTCCGTGGATCACATCATGCTGG
CAGGCGGCACGGCTTCGATTTCAGGGCTGGAGCATTTGATTCAACGACGCATTGGCACCCCGACGATGGTCGCCAACCCG
TTTGCCGACATGGCCCTGAGCTCCAAGGTCAATGCCGGGGCGCTGGCCAGTGACGCTCCCGCGTTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z6Y2Y4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

53.672

100

0.537
  pilM Legionella pneumophila strain ERS1305867

46.761

100

0.469

Multiple sequence alignment