Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CDG61_RS15740 Genome accession   NZ_CP032143
Coordinates   3224211..3225923 (+) Length   570 a.a.
NCBI ID   WP_087550411.1    Uniprot ID   -
Organism   Acinetobacter sp. WCHAc010052     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3219211..3230923
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDG61_RS15700 (CDG61_15700) nusA 3219948..3221432 (-) 1485 WP_087550415.1 transcription termination factor NusA -
  CDG61_RS15705 (CDG61_15705) rimP 3221469..3221993 (-) 525 WP_087550414.1 ribosome maturation factor RimP -
  CDG61_RS15730 (CDG61_15730) secG 3222788..3223120 (-) 333 WP_087550413.1 preprotein translocase subunit SecG -
  CDG61_RS15735 (CDG61_15735) tpiA 3223134..3223925 (-) 792 WP_087550412.1 triose-phosphate isomerase -
  CDG61_RS15740 (CDG61_15740) pilB 3224211..3225923 (+) 1713 WP_087550411.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CDG61_RS15745 (CDG61_15745) pilC 3225952..3227178 (+) 1227 WP_087550410.1 type II secretion system F family protein Machinery gene
  CDG61_RS15750 (CDG61_15750) pilD 3227178..3228038 (+) 861 WP_087550409.1 prepilin peptidase Machinery gene
  CDG61_RS15755 (CDG61_15755) coaE 3228053..3228652 (+) 600 WP_087550408.1 dephospho-CoA kinase -
  CDG61_RS15760 (CDG61_15760) - 3228654..3229568 (-) 915 WP_087550407.1 DMT family transporter -
  CDG61_RS15765 (CDG61_15765) rlmB 3229603..3230352 (-) 750 WP_087550406.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  CDG61_RS15770 (CDG61_15770) - 3230484..3230807 (-) 324 WP_087512620.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63357.52 Da        Isoelectric Point: 4.8792

>NTDB_id=313876 CDG61_RS15740 WP_087550411.1 3224211..3225923(+) (pilB) [Acinetobacter sp. WCHAc010052]
MSVLQGSPKFTGFIRRLVDEGTVSAEKMQQAVAGAKKAKQDIVPYLIEQLSLSPKTVAEKISEEFAEPIFDLAAYDTAQI
IREGIDDKLITRHRILPVFKRGHILYVATSNPTNMDAMDAIRFNSKMNIEAIIVEHNKLEKIIEQNFAEESTFDFSDDDI
DLDLDEGTSPQTTDDDDEPQADEAPIVKYINKLLIDAIRMGASDLHFEPYEKTYRVRYRVDGVLRQIATPPLQLSNRLAS
RLKVMSQMDISEKRIPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKALFMEALEKP
QGMLLITGPTGSGKTVSLYTGLNILNQEDTNISTAEDPVEINLEGVNQVNVNPKVGLTFAVALKSFLRQDPDIVMVGEIR
DLETAEIAVKAAQTGHMVLSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSHCKEPVDVPRNSLLELGF
TEEDLNNSEFQVFQPVGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNAIKIAEASEKSGFNNLRRSGLVKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=313876 CDG61_RS15740 WP_087550411.1 3224211..3225923(+) (pilB) [Acinetobacter sp. WCHAc010052]
ATGTCAGTATTACAGGGTTCACCTAAATTCACAGGATTTATTCGTCGCCTCGTCGATGAAGGGACAGTTTCTGCCGAAAA
AATGCAGCAGGCTGTTGCAGGAGCAAAAAAAGCCAAACAGGATATTGTTCCCTATCTGATTGAACAGCTCAGCCTTTCTC
CCAAAACTGTTGCTGAAAAAATTTCAGAAGAGTTCGCAGAACCCATTTTTGATCTTGCTGCCTATGATACAGCTCAGATT
ATCCGTGAAGGCATAGACGATAAGCTGATCACACGCCACCGTATTCTGCCTGTTTTCAAACGGGGTCACATCCTGTACGT
TGCAACCAGCAATCCAACCAATATGGATGCCATGGATGCGATCCGCTTCAACAGTAAAATGAATATTGAAGCGATTATTG
TTGAACACAATAAACTCGAAAAAATTATTGAACAGAATTTCGCTGAAGAAAGTACTTTTGATTTTTCAGATGATGATATT
GACCTGGATCTTGATGAGGGTACCTCCCCCCAGACTACAGATGACGATGATGAACCACAGGCAGATGAAGCCCCAATCGT
AAAATATATCAATAAATTACTGATTGATGCCATACGTATGGGTGCATCGGATTTACATTTTGAACCCTATGAAAAAACCT
ATCGTGTCCGCTACCGTGTAGATGGTGTACTGCGCCAGATTGCGACACCACCCTTACAGTTGTCAAACCGTCTTGCCTCC
CGACTGAAAGTCATGTCACAGATGGATATTTCTGAAAAAAGGATTCCTCAGGACGGACGTATCAAACTGAAACTGTCCAA
AACCAAAGCCATCGACTTCCGTGTCAACTCATTACCGACACTGTTTGGTGAGAAACTGGTTCTGCGTATCCTGGATCCAT
CCAGTGCCATGCTGGGCATTGATGCTTTAGGATATGAACCTGAACAGAAAGCCCTGTTTATGGAAGCTCTGGAAAAACCT
CAGGGAATGTTACTGATCACAGGTCCAACCGGTTCGGGTAAAACGGTTTCTCTTTATACCGGTCTGAATATTCTGAACCA
GGAAGACACCAACATCTCCACAGCCGAAGACCCTGTCGAAATTAACCTTGAAGGGGTTAACCAGGTGAACGTCAACCCCA
AAGTTGGTCTGACCTTCGCCGTCGCACTTAAATCATTCCTGCGTCAGGATCCTGACATTGTTATGGTCGGTGAGATCCGG
GATCTTGAAACAGCTGAAATAGCAGTCAAAGCCGCACAGACAGGACACATGGTTCTTTCCACGCTGCACACCAACAGTGC
ACCTGAAACATTGACCCGTTTGCGGAATATGGGGGTTCCATCCTTCAACATTGCAACCTCTGTCAACCTGGTGATTGCTC
AGCGACTGGCCCGTCGCTTATGTTCACACTGCAAGGAACCTGTTGATGTTCCACGTAACAGCTTACTGGAACTGGGTTTC
ACAGAAGAAGATCTGAATAATTCAGAATTCCAGGTTTTTCAGCCAGTCGGCTGTTCAGAATGCCGTGAAGGTTATAAGGG
TCGTGTCGGTATTTATGAAGTTATGAAAGTTACACCAGAAATTTCAAAGATTATTATGGAAGATGGTAATGCGATCAAAA
TTGCAGAAGCATCTGAAAAATCGGGGTTTAATAATCTTCGCCGCTCTGGATTAGTGAAAGTCATGCAGGGGGTTACTTCT
CTTCAGGAAGTAAATCGTGTGACGAGTGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.877

100

0.809
  pilB Acinetobacter baylyi ADP1

77.273

100

0.775
  pilB Legionella pneumophila strain ERS1305867

52.364

96.491

0.505
  pilF Neisseria gonorrhoeae MS11

50.178

98.596

0.495
  pilB Vibrio cholerae strain A1552

49.639

97.193

0.482
  pilB Vibrio campbellii strain DS40M4

50.382

91.93

0.463
  pilB Vibrio parahaemolyticus RIMD 2210633

50.287

91.754

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

34.202

100

0.368

Multiple sequence alignment