Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CDG60_RS02500 Genome accession   NZ_CP032134
Coordinates   359649..361361 (-) Length   570 a.a.
NCBI ID   WP_087512626.1    Uniprot ID   A0A3B7LSC4
Organism   Acinetobacter chinensis strain WCHAc010005     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 354649..366361
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDG60_RS02470 (CDG60_02470) - 354765..355088 (+) 324 WP_087512620.1 pyrimidine/purine nucleoside phosphorylase -
  CDG60_RS02475 (CDG60_02475) rlmB 355220..355969 (+) 750 WP_087512621.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  CDG60_RS02480 (CDG60_02480) - 356004..356918 (+) 915 WP_087512622.1 DMT family transporter -
  CDG60_RS02485 (CDG60_02485) coaE 356920..357519 (-) 600 WP_087512623.1 dephospho-CoA kinase -
  CDG60_RS02490 (CDG60_02490) pilD 357534..358394 (-) 861 WP_087512624.1 A24 family peptidase Machinery gene
  CDG60_RS02495 (CDG60_02495) pilC 358394..359620 (-) 1227 WP_087512625.1 type II secretion system F family protein Machinery gene
  CDG60_RS02500 (CDG60_02500) pilB 359649..361361 (-) 1713 WP_087512626.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CDG60_RS02505 (CDG60_02505) tpiA 361647..362438 (+) 792 WP_087512627.1 triose-phosphate isomerase -
  CDG60_RS02510 (CDG60_02510) secG 362452..362784 (+) 333 WP_087512628.1 preprotein translocase subunit SecG -
  CDG60_RS02535 (CDG60_02535) rimP 363579..364103 (+) 525 WP_087512629.1 ribosome maturation factor RimP -
  CDG60_RS02540 (CDG60_02540) nusA 364140..365624 (+) 1485 WP_087512630.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63354.56 Da        Isoelectric Point: 4.8792

>NTDB_id=313817 CDG60_RS02500 WP_087512626.1 359649..361361(-) (pilB) [Acinetobacter chinensis strain WCHAc010005]
MSVLQGSPKFTGFIRRLVDEGTVSAEKMQQAVAGAKKAKQDIVPYLIEQLSLSPKTVAEKISEEFAEPIFDLAAYDTAQI
IREGIDDKLITRHRILPVFKRGHILYVATSNPTNMDAMDAIRFNSKMNIEAIIVEHDKLEKIIEQNFAEESTFDFSDDDI
DLDLDEGTSPQTTDTDDEPQADEAPIVKYINKLLIDAIRMGASDLHFEPYEKTYRVRYRVDGVLRQIATPPLQLSNRLAS
RLKVMSQMDISEKRIPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKALFMEALEKP
QGMLLITGPTGSGKTVSLYTGLNILNQEDTNISTAEDPVEINLEGVNQVNVNPKVGLTFAVALKSFLRQDPDIVMVGEIR
DLETAEIAVKAAQTGHMVLSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSHCKEPVDVPRNSLLELGF
TEEDLNNPEFQVFQPVGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNAIKIAEASEKSGFNNLRRSGLVKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=313817 CDG60_RS02500 WP_087512626.1 359649..361361(-) (pilB) [Acinetobacter chinensis strain WCHAc010005]
ATGTCAGTATTACAGGGTTCACCTAAATTCACAGGATTTATTCGTCGCCTCGTCGATGAAGGAACAGTTTCTGCCGAAAA
AATGCAGCAGGCTGTTGCAGGAGCAAAAAAAGCCAAACAGGATATTGTTCCCTATCTGATTGAACAGCTCAGCCTTTCTC
CCAAAACTGTTGCTGAAAAAATTTCAGAAGAGTTCGCAGAACCCATTTTTGATCTTGCTGCCTATGATACAGCTCAGATT
ATCCGTGAAGGCATAGACGATAAGCTGATCACACGCCACCGTATTCTGCCTGTTTTTAAACGCGGTCACATCCTGTACGT
TGCAACCAGCAATCCAACCAACATGGATGCCATGGATGCGATCCGCTTCAACAGTAAAATGAATATCGAAGCGATTATTG
TTGAACACGATAAACTCGAAAAAATTATTGAACAGAATTTCGCTGAAGAAAGTACTTTTGATTTTTCAGATGATGATATT
GACCTGGATCTTGATGAGGGCACCTCCCCTCAGACTACAGATACCGATGATGAACCACAGGCAGATGAAGCCCCAATCGT
CAAATATATCAATAAATTACTGATTGACGCCATACGTATGGGGGCTTCGGATTTACATTTTGAACCCTATGAAAAAACCT
ATCGTGTCCGCTACCGGGTAGATGGTGTACTGCGCCAGATTGCGACCCCCCCCTTACAGTTGTCAAACCGTCTTGCCTCC
CGACTGAAAGTCATGTCACAGATGGATATTTCTGAAAAACGGATTCCTCAGGATGGCCGTATCAAACTGAAACTGTCCAA
AACCAAAGCCATCGACTTCCGTGTCAACTCATTACCGACACTGTTTGGTGAGAAACTAGTTCTGCGTATCCTGGACCCAT
CCAGTGCCATGCTGGGCATTGATGCTTTAGGTTATGAACCTGAACAGAAAGCCCTGTTCATGGAAGCTTTGGAAAAACCT
CAGGGAATGTTACTGATCACAGGTCCAACCGGTTCAGGTAAAACGGTTTCTCTTTATACCGGTCTGAATATTCTGAACCA
GGAAGACACCAATATCTCCACAGCCGAAGACCCTGTCGAAATTAACCTTGAAGGGGTTAACCAGGTGAACGTCAACCCTA
AAGTTGGTCTGACCTTCGCCGTCGCACTTAAATCATTCCTACGTCAGGATCCTGACATTGTTATGGTCGGTGAGATCCGG
GATCTTGAAACAGCTGAAATTGCAGTCAAAGCCGCACAGACAGGACACATGGTTCTTTCCACGCTGCACACCAACAGCGC
ACCTGAAACATTGACCCGTTTGCGGAATATGGGGGTTCCATCCTTCAACATTGCAACCTCTGTCAACCTGGTGATTGCTC
AGCGTCTGGCCCGCCGGTTATGTTCACACTGTAAAGAGCCTGTTGATGTTCCACGTAACAGCTTACTGGAACTGGGTTTC
ACAGAAGAAGACCTCAACAATCCAGAGTTCCAGGTTTTTCAGCCAGTCGGCTGTTCAGAATGCCGTGAAGGTTATAAGGG
TCGTGTCGGTATTTATGAAGTTATGAAAGTTACACCAGAAATTTCAAAGATTATTATGGAAGATGGTAATGCGATTAAAA
TTGCAGAAGCATCTGAAAAATCGGGGTTTAATAATCTTCGCCGCTCTGGATTAGTGAAAGTCATGCAGGGGGTTACTTCG
CTTCAGGAAGTAAATCGTGTGACGAGTGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3B7LSC4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.053

100

0.811
  pilB Acinetobacter baylyi ADP1

77.448

100

0.777
  pilB Legionella pneumophila strain ERS1305867

52.182

96.491

0.504
  pilF Neisseria gonorrhoeae MS11

50.356

98.596

0.496
  pilB Vibrio cholerae strain A1552

49.91

97.368

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

50.287

91.754

0.461
  pilB Vibrio campbellii strain DS40M4

50.577

91.228

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

34.365

100

0.37
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.795

93.158

0.361

Multiple sequence alignment