Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   Ahae5227_RS01480 Genome accession   NZ_CP031988
Coordinates   320086..322218 (+) Length   710 a.a.
NCBI ID   WP_171479087.1    Uniprot ID   -
Organism   Acinetobacter haemolyticus strain 5227     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 315086..327218
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Ahae5227_RS01460 (Ahae5227_01480) comM 317088..318146 (+) 1059 WP_004641327.1 pilus assembly protein PilM Machinery gene
  Ahae5227_RS01465 (Ahae5227_01485) pilN 318146..318790 (+) 645 WP_004641325.1 PilN domain-containing protein Machinery gene
  Ahae5227_RS01470 (Ahae5227_01490) pilO 318787..319497 (+) 711 WP_008941136.1 type 4a pilus biogenesis protein PilO Machinery gene
  Ahae5227_RS01475 (Ahae5227_01495) pilP 319497..320024 (+) 528 WP_005088514.1 pilus assembly protein PilP Machinery gene
  Ahae5227_RS01480 (Ahae5227_01500) pilQ 320086..322218 (+) 2133 WP_171479087.1 type IV pilus secretin PilQ family protein Machinery gene
  Ahae5227_RS01485 (Ahae5227_01505) aroK 322257..322799 (+) 543 WP_005087029.1 shikimate kinase AroK -
  Ahae5227_RS01490 (Ahae5227_01510) aroB 322820..323905 (+) 1086 WP_008941139.1 3-dehydroquinate synthase -
  Ahae5227_RS01495 (Ahae5227_01515) - 323921..324793 (+) 873 WP_005088508.1 hypothetical protein -

Sequence

Protein


Download         Length: 710 a.a.        Molecular weight: 77118.96 Da        Isoelectric Point: 7.2181

>NTDB_id=313159 Ahae5227_RS01480 WP_171479087.1 320086..322218(+) (pilQ) [Acinetobacter haemolyticus strain 5227]
MNRAFRQFSMGAVAIAVMQVASAQVNMTNIVPMSLADQGTEIRVMFNGLPPQPQAYQLEQPSRLILDFDKAKQTLKQSSI
PVATNEASSVEVAADDQRSRVIVNLKDAGAFTTRVEGNTFILKINSARPAIASPVSATARQVQQGVSNIGFQRGNQGEGL
VVIDLLGSNTPVDVQQQGSKIVVRTMGNKIPTHLARRLNTNDFATPVASIDAYNDKGNGVITIQSAGSYEYMAYQAENKL
TISLKRPEEKSPLKAKSQTYTGNKISLDFQDIEVRRVLQLLADFTNINMVAADTVQGNITLRLKDVPWDQALDIVLKTKN
LDKRRNGNVIWIAPVAELIKAEEEEAKAIAQSVKLAPIQSEYIKLSYAKAADILKLIEDSRDGKAGASNRTANSDSLALE
SLLSPRGSAVADVRTNTLIVNDTAQNIDKIRKMVDLLDVSVKQVMIEARIVRASTDFTKEMGVKWGILSQGITRNNDLLV
GGSDTTLWNLREPKKDSETGGWKYEIERPDNLNVDLGAVTPGASRIAFGLISLSDFMLDLELSAVQADGFGEVISTPKVM
TADKQPAKIESGTKIPYASSTGGGANAVPKTEFIDATLSLDVTPSITPDGKVMMKLNIAKDAPGAPTPTGQLTISKNSID
TNVLVDNGETVVLGGIFEQENLSSQIKVPFLGDLPYVGRLFRRDLKTDNKRELLIFVTPRIVNDTLTRNH

Nucleotide


Download         Length: 2133 bp        

>NTDB_id=313159 Ahae5227_RS01480 WP_171479087.1 320086..322218(+) (pilQ) [Acinetobacter haemolyticus strain 5227]
ATGAATCGTGCATTCCGTCAATTTTCAATGGGTGCTGTCGCAATAGCAGTGATGCAGGTAGCATCAGCACAAGTGAACAT
GACCAACATTGTGCCAATGAGTTTGGCTGATCAGGGGACTGAAATTCGTGTCATGTTTAATGGTCTGCCACCACAGCCGC
AGGCATATCAACTTGAACAACCCTCTCGTTTGATTTTGGATTTTGATAAAGCTAAACAAACCTTAAAGCAGAGTTCTATA
CCTGTTGCAACCAATGAAGCCAGTTCAGTAGAAGTCGCTGCGGATGACCAGCGTTCTCGCGTGATTGTGAATTTGAAAGA
TGCAGGCGCTTTTACCACACGTGTAGAAGGTAATACTTTTATTCTAAAAATTAATTCTGCGCGTCCTGCTATTGCGAGTC
CTGTATCTGCCACTGCCCGTCAAGTACAACAAGGGGTTTCAAATATTGGTTTCCAACGTGGCAATCAAGGTGAAGGTCTA
GTTGTGATTGATTTATTGGGTAGTAATACGCCAGTAGATGTACAACAGCAAGGGAGTAAAATCGTTGTGCGGACGATGGG
GAACAAAATTCCGACCCATTTAGCACGTCGTTTAAATACCAATGATTTCGCAACTCCTGTAGCAAGTATTGATGCCTATA
ATGATAAAGGTAATGGTGTGATTACCATTCAGTCTGCGGGTAGTTATGAATACATGGCTTATCAAGCTGAAAATAAACTG
ACGATTAGTTTAAAGCGACCTGAAGAAAAATCACCATTAAAAGCAAAATCTCAAACTTATACTGGCAATAAGATTTCTTT
AGATTTCCAAGATATTGAAGTGCGTCGTGTGCTGCAGTTACTTGCTGATTTTACCAATATCAATATGGTGGCTGCTGATA
CGGTACAAGGGAATATTACTTTACGCCTAAAAGATGTACCTTGGGATCAGGCTTTGGATATCGTGCTGAAAACCAAAAAC
TTGGATAAGCGTCGTAATGGTAATGTGATTTGGATTGCACCAGTGGCAGAACTGATCAAGGCAGAGGAAGAAGAGGCAAA
AGCCATCGCTCAGAGTGTGAAGCTCGCGCCGATCCAATCAGAGTATATCAAGCTGAGTTATGCCAAAGCGGCGGATATCT
TAAAGCTGATTGAAGATTCTCGTGACGGTAAGGCTGGGGCATCAAATCGTACTGCGAATTCAGATTCACTCGCACTGGAA
AGTTTGTTAAGTCCACGTGGTAGTGCAGTGGCTGATGTACGAACCAATACTTTAATTGTAAATGATACTGCTCAAAATAT
TGACAAGATCCGTAAGATGGTTGATTTGCTGGATGTCTCTGTTAAGCAAGTTATGATCGAAGCCCGCATTGTTCGAGCAA
GCACTGACTTTACGAAGGAAATGGGGGTGAAGTGGGGAATCTTGTCTCAAGGTATCACTCGAAATAATGATTTGCTGGTT
GGTGGTAGTGATACTACGTTGTGGAATCTAAGAGAGCCTAAGAAAGACAGTGAGACTGGTGGGTGGAAGTATGAAATCGA
ACGTCCTGATAATTTAAATGTGGATTTGGGGGCAGTTACTCCGGGTGCAAGTCGTATTGCTTTTGGCTTAATTAGCCTCT
CTGATTTTATGTTGGATTTAGAACTTTCTGCAGTACAGGCGGATGGTTTTGGTGAGGTTATTTCAACGCCTAAAGTGATG
ACTGCTGATAAGCAACCTGCAAAGATCGAGTCAGGAACCAAAATTCCTTATGCATCTTCGACGGGTGGGGGCGCAAATGC
GGTACCTAAAACGGAGTTCATTGATGCAACTTTAAGCTTAGATGTGACTCCAAGCATTACGCCCGATGGAAAAGTCATGA
TGAAACTTAACATTGCAAAAGATGCACCTGGCGCACCAACGCCAACGGGTCAATTAACCATTAGTAAGAATTCAATTGAT
ACCAATGTTTTGGTGGATAATGGTGAAACTGTGGTGCTCGGCGGTATTTTTGAGCAGGAAAATCTCAGCTCACAAATTAA
AGTGCCATTTTTAGGTGATCTTCCGTATGTGGGTCGACTTTTCCGTCGTGATCTGAAAACAGACAATAAACGTGAACTGC
TTATTTTTGTGACACCGAGAATTGTTAATGACACTTTGACCAGAAATCATTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(292-334)

Bacterial type II/III secretion system short domain (Secretin_N)

(362-443)

AMIN domain (AMIN)

(37-121)

Bacterial type II and III secretion system protein (Secretin)

(544-702)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Acinetobacter baumannii D1279779

75.138

100

0.766
  pilQ Acinetobacter baumannii strain A118

75

100

0.765
  comQ Acinetobacter baylyi ADP1

70.99

100

0.717
  pilQ Pseudomonas aeruginosa PAK

39.538

100

0.41
  pilQ Legionella pneumophila strain ERS1305867

39.021

94.93

0.37
  pilQ Legionella pneumophila strain Lp02

38.872

94.93

0.369

Multiple sequence alignment