Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   Ahae5227_RS01470 Genome accession   NZ_CP031988
Coordinates   318787..319497 (+) Length   236 a.a.
NCBI ID   WP_008941136.1    Uniprot ID   C0VN50
Organism   Acinetobacter haemolyticus strain 5227     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 313787..324497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Ahae5227_RS01455 (Ahae5227_01475) ponA 314333..316924 (-) 2592 WP_004641329.1 penicillin-binding protein PBP1a -
  Ahae5227_RS01460 (Ahae5227_01480) comM 317088..318146 (+) 1059 WP_004641327.1 pilus assembly protein PilM Machinery gene
  Ahae5227_RS01465 (Ahae5227_01485) pilN 318146..318790 (+) 645 WP_004641325.1 PilN domain-containing protein Machinery gene
  Ahae5227_RS01470 (Ahae5227_01490) pilO 318787..319497 (+) 711 WP_008941136.1 type 4a pilus biogenesis protein PilO Machinery gene
  Ahae5227_RS01475 (Ahae5227_01495) pilP 319497..320024 (+) 528 WP_005088514.1 pilus assembly protein PilP Machinery gene
  Ahae5227_RS01480 (Ahae5227_01500) pilQ 320086..322218 (+) 2133 WP_171479087.1 type IV pilus secretin PilQ family protein Machinery gene
  Ahae5227_RS01485 (Ahae5227_01505) aroK 322257..322799 (+) 543 WP_005087029.1 shikimate kinase AroK -
  Ahae5227_RS01490 (Ahae5227_01510) aroB 322820..323905 (+) 1086 WP_008941139.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 236 a.a.        Molecular weight: 26973.56 Da        Isoelectric Point: 4.8810

>NTDB_id=313157 Ahae5227_RS01470 WP_008941136.1 318787..319497(+) (pilO) [Acinetobacter haemolyticus strain 5227]
MSQDELNDLNQEGAAVKKKKMTVEQFFQQFNTLDMNNYGSWPWSVKVTCWIFILLAVVALGYFVVIQKQLQTIANAEARE
QSLLNEFREKESKLRNLQQYQTQLQEMEANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEKEVRQEFFIEQP
ISLEATGDYHAFGAFATGIAALPRIVTLHDFIVEAKEDTQKKSDIPVVHYTVKAKTYRYVGADDQVDSTAVSGGQR

Nucleotide


Download         Length: 711 bp        

>NTDB_id=313157 Ahae5227_RS01470 WP_008941136.1 318787..319497(+) (pilO) [Acinetobacter haemolyticus strain 5227]
ATGAGTCAAGATGAACTAAATGATCTCAATCAAGAAGGCGCAGCTGTAAAGAAAAAGAAAATGACAGTTGAGCAATTTTT
TCAGCAATTTAATACCTTAGATATGAACAACTATGGCAGTTGGCCATGGTCGGTAAAAGTGACGTGTTGGATTTTTATTT
TACTCGCTGTTGTGGCATTGGGTTATTTCGTTGTGATCCAAAAGCAGCTACAGACGATAGCTAATGCTGAAGCGCGTGAA
CAAAGTCTATTAAATGAGTTTAGAGAAAAGGAATCTAAATTACGCAACTTGCAGCAATACCAAACTCAATTGCAAGAAAT
GGAAGCGAATTTTAATCAGCAGCTTGAGCAGTTGCCAAAAGAAACTGAAATTCCGAGTTTGGTTGAGGATATAAACTTAA
CTGGGGTAAATTCGGGTCTGAAATTTAAAAATATTCGTTTAGAAAAAGAAGTTAGACAAGAGTTCTTCATTGAGCAACCA
ATTAGTCTGGAAGCAACAGGCGATTATCATGCCTTCGGTGCTTTTGCGACAGGTATTGCCGCTTTGCCTCGAATCGTGAC
TTTGCATGATTTTATCGTCGAAGCGAAAGAAGATACTCAGAAAAAATCTGATATCCCTGTTGTCCATTATACGGTTAAAG
CAAAAACTTATCGTTATGTTGGGGCGGATGATCAGGTTGATAGCACTGCTGTTTCAGGGGGGCAACGCTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C0VN50

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

74.348

97.458

0.725
  comO Acinetobacter baylyi ADP1

71.861

97.881

0.703

Multiple sequence alignment