Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   D1100_RS04840 Genome accession   NZ_CP031869
Coordinates   1050991..1052385 (-) Length   464 a.a.
NCBI ID   WP_005601209.1    Uniprot ID   B0BPG4
Organism   Actinobacillus pleuropneumoniae strain WF83     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1045991..1057385
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1100_RS04810 (D1100_04825) lptE 1046605..1047099 (+) 495 WP_005597385.1 LPS assembly lipoprotein LptE -
  D1100_RS04815 (D1100_04830) holA 1047208..1048233 (+) 1026 WP_005612206.1 DNA polymerase III subunit delta -
  D1100_RS04820 (D1100_04835) yacG 1048310..1048498 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  D1100_RS04825 (D1100_04840) coaE 1048480..1049118 (-) 639 WP_005617375.1 dephospho-CoA kinase -
  D1100_RS04830 (D1100_04845) - 1049140..1049778 (-) 639 WP_012478451.1 prepilin peptidase -
  D1100_RS04835 (D1100_04850) pilC 1049805..1051007 (-) 1203 WP_005617379.1 type II secretion system F family protein Machinery gene
  D1100_RS04840 (D1100_04855) pilB 1050991..1052385 (-) 1395 WP_005601209.1 GspE/PulE family protein Machinery gene
  D1100_RS04845 (D1100_04860) pilA 1052403..1052849 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  D1100_RS04850 (D1100_04865) radA 1053031..1054410 (+) 1380 WP_017357656.1 DNA repair protein RadA -
  D1100_RS04855 (D1100_04870) - 1054454..1054687 (-) 234 WP_005615190.1 YgjV family protein -
  D1100_RS04860 (D1100_04875) folA 1054814..1055302 (-) 489 WP_005597400.1 type 3 dihydrofolate reductase -
  D1100_RS04865 (D1100_04880) proB 1055389..1056480 (+) 1092 WP_005601213.1 glutamate 5-kinase -
  D1100_RS04870 (D1100_04885) - 1056493..1057269 (+) 777 WP_005597402.1 TSUP family transporter -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 53022.90 Da        Isoelectric Point: 6.5042

>NTDB_id=312207 D1100_RS04840 WP_005601209.1 1050991..1052385(-) (pilB) [Actinobacillus pleuropneumoniae strain WF83]
MQYSVCDVNNRQVFRISEEQWQKNYDERHILLRYLTVPLREDQQTLWLAIDDMNNLSACEIFAFLTHKRIEPVLVSSEEL
KYLLGQLMPEQQSVYDETELPYQPLAQAQHSDVNDPIIQVLDGLFKYGLKHNASDIHLEPQKDKMLIRLRIDGVLHPYKS
FSLQLANRLISRIKLLAKLDISESRLPQDGQFGFKTILSETLDFRVSSLPTYWGEKVVLRLQKNKPTHFDFTTLGFLAEQ
KEKLLRALRQPQGLILVTGPTGSGKSITLYSALDYLNDSKRHILTAEDPIEIELEGIIQTQINPAIDLGFSRLLRTFLRQ
DPDVIMLGEIRDQESAEIALSAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEINNSLLLVVAQRLVRKLCTECGGKGC
KYCYHGYRGRIGVYQLFSKNGKIFEKSTACLDFENLYQSAQQKFKTGITSLEEIERVLGHVESV

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=312207 D1100_RS04840 WP_005601209.1 1050991..1052385(-) (pilB) [Actinobacillus pleuropneumoniae strain WF83]
ATGCAGTATTCCGTATGTGATGTAAACAATCGGCAAGTATTCCGGATTTCCGAGGAACAGTGGCAAAAAAATTATGATGA
ACGTCATATCTTATTACGTTATCTCACCGTACCGCTTCGGGAAGACCAACAAACATTATGGTTGGCAATCGATGATATGA
ATAATCTCAGTGCTTGCGAGATTTTTGCTTTTCTTACGCATAAGCGAATTGAGCCGGTATTGGTCTCTTCCGAAGAATTG
AAATACCTGCTCGGGCAATTAATGCCGGAACAACAATCGGTATATGACGAAACGGAATTACCTTATCAACCTTTAGCACA
AGCGCAGCATTCGGATGTTAATGATCCGATTATTCAAGTGTTGGACGGCCTGTTTAAATACGGTTTAAAACATAATGCTT
CGGATATTCATTTGGAACCGCAAAAAGACAAAATGTTGATTCGATTACGTATCGACGGCGTGTTACATCCGTATAAATCA
TTTTCTCTCCAACTTGCCAATCGACTTATTTCTCGCATTAAATTATTAGCTAAACTGGATATTAGTGAATCCCGTCTTCC
GCAAGACGGGCAATTCGGTTTTAAGACAATCTTATCCGAAACATTGGATTTCCGTGTTTCGAGTTTACCGACTTATTGGG
GCGAAAAAGTCGTTTTACGGCTACAAAAAAATAAACCGACGCATTTTGATTTTACTACGTTGGGCTTCTTGGCGGAGCAG
AAGGAAAAGTTGTTGCGGGCATTGCGGCAGCCTCAAGGATTAATATTAGTTACGGGACCGACCGGCAGTGGTAAAAGTAT
TACCTTATATAGTGCGTTGGATTATTTAAATGACAGTAAAAGGCATATTTTGACCGCCGAAGATCCGATAGAAATCGAAC
TTGAAGGCATAATCCAAACGCAAATAAACCCGGCGATAGATTTAGGGTTCAGCCGGCTATTAAGAACGTTTTTACGACAA
GATCCGGATGTGATTATGTTAGGTGAAATTCGTGATCAAGAAAGTGCCGAAATTGCATTAAGTGCGGCACAAACCGGGCA
TTTAGTGCTTTCGACCTTACATACCAATGATGCGCCTTCGGCAATCGAACGCTTATTGCAGCTCGGTATCAAAGAATATG
AAATCAATAATTCGTTATTACTTGTCGTGGCGCAACGTTTAGTTCGTAAATTATGTACCGAATGCGGCGGAAAAGGCTGT
AAATATTGTTATCACGGCTACAGAGGCAGAATCGGCGTTTATCAACTGTTTTCAAAAAATGGAAAAATTTTTGAGAAATC
GACCGCTTGTTTGGATTTTGAAAACTTATATCAAAGTGCGCAACAGAAATTCAAGACGGGAATCACTTCATTAGAAGAAA
TCGAGAGAGTGTTAGGTCATGTTGAAAGTGTATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(28-92)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B0BPG4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

68.192

98.922

0.675
  pilB Haemophilus influenzae Rd KW20

58.72

97.629

0.573
  pilB Haemophilus influenzae 86-028NP

58.278

97.629

0.569
  pilB Vibrio campbellii strain DS40M4

40.125

100

0.416
  pilB Legionella pneumophila strain ERS1305867

39.264

100

0.414
  pilB Vibrio parahaemolyticus RIMD 2210633

39.709

100

0.412
  pilB Acinetobacter baumannii D1279779

38.855

100

0.409
  pilB Vibrio cholerae strain A1552

39.004

100

0.405
  pilB Acinetobacter baylyi ADP1

38.32

100

0.403
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.538

100

0.369

Multiple sequence alignment