Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   D1100_RS04835 Genome accession   NZ_CP031869
Coordinates   1049805..1051007 (-) Length   400 a.a.
NCBI ID   WP_005617379.1    Uniprot ID   B3H1L7
Organism   Actinobacillus pleuropneumoniae strain WF83     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1044805..1056007
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1100_RS04810 (D1100_04825) lptE 1046605..1047099 (+) 495 WP_005597385.1 LPS assembly lipoprotein LptE -
  D1100_RS04815 (D1100_04830) holA 1047208..1048233 (+) 1026 WP_005612206.1 DNA polymerase III subunit delta -
  D1100_RS04820 (D1100_04835) yacG 1048310..1048498 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  D1100_RS04825 (D1100_04840) coaE 1048480..1049118 (-) 639 WP_005617375.1 dephospho-CoA kinase -
  D1100_RS04830 (D1100_04845) - 1049140..1049778 (-) 639 WP_012478451.1 prepilin peptidase -
  D1100_RS04835 (D1100_04850) pilC 1049805..1051007 (-) 1203 WP_005617379.1 type II secretion system F family protein Machinery gene
  D1100_RS04840 (D1100_04855) pilB 1050991..1052385 (-) 1395 WP_005601209.1 GspE/PulE family protein Machinery gene
  D1100_RS04845 (D1100_04860) pilA 1052403..1052849 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  D1100_RS04850 (D1100_04865) radA 1053031..1054410 (+) 1380 WP_017357656.1 DNA repair protein RadA -
  D1100_RS04855 (D1100_04870) - 1054454..1054687 (-) 234 WP_005615190.1 YgjV family protein -
  D1100_RS04860 (D1100_04875) folA 1054814..1055302 (-) 489 WP_005597400.1 type 3 dihydrofolate reductase -

Sequence

Protein


Download         Length: 400 a.a.        Molecular weight: 45594.13 Da        Isoelectric Point: 10.3238

>NTDB_id=312206 D1100_RS04835 WP_005617379.1 1049805..1051007(-) (pilC) [Actinobacillus pleuropneumoniae strain WF83]
MLKVYEFHWKAKNRFQQLQKGKALACHRTELERRLLAKGYSQIRIQRNFRFVTQASSESITQVIKQLALLLNAAVPLKQA
LNMLLENTYHIRIYMWLNDLNRLIESGYTMSSSLTKLNLYLAKSEIQLVRIGEQSGRLGEILQNIAVNRHQTEKLHKKIK
KIMFYPVVTLIISVALSLAMLLFIVPQFIELYSGREKPLPLITQMLFTLSLFLQHSLLQVTIVSLLAVGFFRFFAKKYRF
LTAIKNGMLSALPIFNKIVENVRIVFFSQNLGLMLKAHIRLETALNTFLATQSQDLRLQKEIENILAQLKQGYRFSESIN
ASVFGTQVVQMLAIGEQSGNVAQMCSYIGELYRQQLDDQIELLSQLLEPILMLIIGLIIGTVLIGLYLPIFDLGGLVGGG

Nucleotide


Download         Length: 1203 bp        

>NTDB_id=312206 D1100_RS04835 WP_005617379.1 1049805..1051007(-) (pilC) [Actinobacillus pleuropneumoniae strain WF83]
ATGTTGAAAGTGTATGAATTCCATTGGAAAGCTAAAAATCGTTTTCAACAACTTCAGAAAGGAAAAGCGTTAGCTTGTCA
TCGAACGGAATTGGAACGGCGTTTATTAGCAAAAGGCTATTCACAGATCCGTATCCAACGTAATTTTCGTTTTGTCACAC
AGGCTTCTTCAGAATCGATTACTCAAGTAATTAAACAGTTGGCGCTTTTACTGAATGCCGCAGTCCCTTTAAAACAAGCG
CTGAATATGTTGTTAGAAAATACTTATCATATTCGGATCTATATGTGGCTGAATGATTTAAACCGTTTGATAGAATCGGG
TTATACAATGTCGTCAAGTTTAACCAAATTGAATTTATATTTGGCGAAATCGGAAATACAGCTGGTACGGATTGGCGAAC
AAAGCGGGCGATTAGGCGAAATTTTGCAAAATATTGCGGTAAATCGCCATCAAACCGAAAAATTACATAAGAAAATAAAA
AAGATAATGTTTTATCCTGTCGTCACTTTGATTATTTCCGTTGCGCTTTCTCTCGCCATGCTCCTTTTTATCGTGCCGCA
ATTTATCGAGCTGTATTCCGGTCGTGAAAAGCCGCTTCCTTTAATTACACAAATGCTATTTACATTATCGCTCTTCTTAC
AACATTCTTTATTACAGGTAACGATAGTCAGTTTATTAGCGGTCGGTTTTTTCCGTTTTTTTGCAAAAAAATATCGATTT
TTAACCGCTATAAAAAACGGGATGTTAAGTGCTTTGCCTATTTTTAATAAGATAGTCGAAAACGTTCGTATAGTATTTTT
TAGTCAAAATCTCGGCTTGATGCTAAAAGCTCATATTCGGCTGGAAACGGCGTTAAATACCTTTCTTGCTACACAATCGC
AAGATTTGAGGTTACAAAAAGAGATAGAAAATATTTTAGCGCAATTAAAACAAGGTTATCGCTTTTCCGAAAGTATTAAT
GCGAGTGTGTTCGGTACTCAAGTAGTACAAATGCTTGCCATTGGCGAACAGAGTGGGAATGTAGCGCAGATGTGTAGCTA
TATCGGGGAGTTATATCGACAACAATTGGATGATCAGATTGAACTGTTATCGCAGCTGCTTGAGCCGATCTTAATGCTGA
TTATCGGATTAATTATCGGTACGGTTTTGATCGGATTATATTTACCGATTTTTGATTTAGGCGGACTAGTCGGAGGCGGT
TAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(267-389)

Type II secretion system (T2SS), protein F (T2SSF)

(64-186)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B3H1L7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Glaesserella parasuis strain SC1401

49.874

99.25

0.495
  pilC Haemophilus influenzae Rd KW20

40

100

0.4
  pilC Haemophilus influenzae 86-028NP

39

100

0.39

Multiple sequence alignment