Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA   Type   Machinery gene
Locus tag   D1100_RS04845 Genome accession   NZ_CP031869
Coordinates   1052403..1052849 (-) Length   148 a.a.
NCBI ID   WP_005597397.1    Uniprot ID   B0BPG5
Organism   Actinobacillus pleuropneumoniae strain WF83     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 1047403..1057849
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1100_RS04820 (D1100_04835) yacG 1048310..1048498 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  D1100_RS04825 (D1100_04840) coaE 1048480..1049118 (-) 639 WP_005617375.1 dephospho-CoA kinase -
  D1100_RS04830 (D1100_04845) - 1049140..1049778 (-) 639 WP_012478451.1 prepilin peptidase -
  D1100_RS04835 (D1100_04850) pilC 1049805..1051007 (-) 1203 WP_005617379.1 type II secretion system F family protein Machinery gene
  D1100_RS04840 (D1100_04855) pilB 1050991..1052385 (-) 1395 WP_005601209.1 GspE/PulE family protein Machinery gene
  D1100_RS04845 (D1100_04860) pilA 1052403..1052849 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  D1100_RS04850 (D1100_04865) radA 1053031..1054410 (+) 1380 WP_017357656.1 DNA repair protein RadA -
  D1100_RS04855 (D1100_04870) - 1054454..1054687 (-) 234 WP_005615190.1 YgjV family protein -
  D1100_RS04860 (D1100_04875) folA 1054814..1055302 (-) 489 WP_005597400.1 type 3 dihydrofolate reductase -
  D1100_RS04865 (D1100_04880) proB 1055389..1056480 (+) 1092 WP_005601213.1 glutamate 5-kinase -
  D1100_RS04870 (D1100_04885) - 1056493..1057269 (+) 777 WP_005597402.1 TSUP family transporter -

Sequence


Protein


Download         Length: 148 a.a.        Molecular weight: 15980.38 Da        Isoelectric Point: 7.6257

>NTDB_id=312208 D1100_RS04845 WP_005597397.1 1052403..1052849(-) (pilA) [Actinobacillus pleuropneumoniae strain WF83]
MQKLSLIRPLTNAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLAASASYKTDVEICIYNTGDSKNCSGGQNGV
RKMTELRQAKYLNAITVEGGTITVTGKGNLQEYGYTMTPIHNGSTISWETKCKGEDLSLFPANFCASN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=312208 D1100_RS04845 WP_005597397.1 1052403..1052849(-) (pilA) [Actinobacillus pleuropneumoniae strain WF83]
ATGCAAAAATTAAGTCTTATTCGACCGCTTACTAACGCGTTTACTTTAATTGAATTGATGATCGTGATTGCGATTATTGC
CATTTTAGCTACGGTTGCAATTCCGTCATATAACAGTTATACCCAAAAAGCGGCGCTTTCGGAGCTATTGGCGGCATCGG
CTTCTTATAAAACGGATGTCGAGATCTGCATATATAACACCGGAGATTCTAAAAACTGTAGCGGCGGTCAAAACGGTGTC
AGAAAAATGACGGAGCTTAGACAGGCTAAATATTTAAATGCCATTACGGTGGAAGGCGGAACGATTACAGTAACGGGGAA
AGGGAATCTACAGGAATACGGTTATACGATGACACCGATTCATAACGGTAGCACTATTTCTTGGGAAACGAAATGTAAAG
GGGAGGACTTAAGTTTATTTCCGGCAAATTTCTGCGCATCAAATTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB B0BPG5

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

68.919

100

0.689

  pilA Haemophilus influenzae 86-028NP

55.102

99.324

0.547

  pilA Haemophilus influenzae Rd KW20

53.061

99.324

0.527


Multiple sequence alignment