Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   D1103_RS04695 Genome accession   NZ_CP031864
Coordinates   1038368..1039762 (-) Length   464 a.a.
NCBI ID   WP_237610862.1    Uniprot ID   -
Organism   Actinobacillus pleuropneumoniae serovar 10 str. D13039     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1033368..1044762
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1103_RS04665 (D1103_04715) lptE 1033982..1034476 (+) 495 WP_005597385.1 LPS assembly lipoprotein LptE -
  D1103_RS04670 (D1103_04720) holA 1034585..1035610 (+) 1026 WP_005612206.1 DNA polymerase III subunit delta -
  D1103_RS04675 (D1103_04725) yacG 1035687..1035875 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  D1103_RS04680 (D1103_04730) coaE 1035857..1036495 (-) 639 WP_005612207.1 dephospho-CoA kinase -
  D1103_RS04685 (D1103_04735) - 1036517..1037155 (-) 639 WP_005604550.1 prepilin peptidase -
  D1103_RS04690 (D1103_04740) pilC 1037182..1038384 (-) 1203 WP_005612209.1 type II secretion system F family protein Machinery gene
  D1103_RS04695 (D1103_04745) pilB 1038368..1039762 (-) 1395 WP_237610862.1 GspE/PulE family protein Machinery gene
  D1103_RS04700 (D1103_04750) pilA 1039780..1040226 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  D1103_RS04705 (D1103_04755) radA 1040408..1041787 (+) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  D1103_RS04710 (D1103_04760) - 1041831..1042064 (-) 234 WP_005597399.1 YgjV family protein -
  D1103_RS04715 (D1103_04765) folA 1042191..1042679 (-) 489 WP_005597400.1 type 3 dihydrofolate reductase -
  D1103_RS04720 (D1103_04770) proB 1042766..1043857 (+) 1092 WP_005601213.1 glutamate 5-kinase -
  D1103_RS04725 (D1103_04775) - 1043870..1044646 (+) 777 WP_005612213.1 TSUP family transporter -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 53090.93 Da        Isoelectric Point: 6.5042

>NTDB_id=312127 D1103_RS04695 WP_237610862.1 1038368..1039762(-) (pilB) [Actinobacillus pleuropneumoniae serovar 10 str. D13039]
MQYSVCDVNNRQVFRISEEQWQKNYDERHILLRYFTVPLREDQQTLWLAIDDMNNLSACEIFAFLTHKRIEPVLVSSEEL
KYLLGQLMPEQQSVYDETELPYQPLAQAQHSDVNDPIIQVLDGLFKYGLKHNASDIHFEPQKDKMLIRLRIDGVLHPYKS
FSLQLANRLISRIKLLAKLDISESRLPQDGQFGFKTILSETLDFRVSSLPTYWGEKVVLRLQKNKPTHFDFTTLGFLAEQ
KEKLLRALRQPQGLILVTGPTGSGKSITLYSALDYLNDSKRHILTAEDPIEIELEGIIQTQINPAIDLGFSRLLRTFLRQ
DPDVIMLGEIRDQESAEIALSAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEINNSLLLVVAQRLVRKLCTECGGKGC
KYCYHGYRGRIGVYQLFSKNGKIFEKSTACLDFENLYQSAQQKFKTGITSLEEIERVLGHVESV

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=312127 D1103_RS04695 WP_237610862.1 1038368..1039762(-) (pilB) [Actinobacillus pleuropneumoniae serovar 10 str. D13039]
ATGCAGTATTCCGTATGTGATGTAAACAATCGGCAAGTATTCCGGATTTCCGAGGAACAGTGGCAAAAAAATTATGATGA
ACGTCATATCTTATTACGTTATTTCACCGTACCGCTTCGGGAAGACCAACAAACATTATGGTTGGCAATCGATGATATGA
ATAATCTCAGTGCTTGCGAGATTTTTGCTTTTCTTACGCATAAGCGAATTGAGCCGGTATTGGTCTCTTCCGAAGAATTG
AAATACCTGCTCGGGCAATTAATGCCGGAACAACAATCGGTATATGACGAAACGGAATTACCTTATCAACCTTTAGCACA
AGCGCAGCATTCGGATGTTAATGATCCGATTATTCAAGTGTTGGACGGCCTGTTTAAATACGGTTTAAAACATAATGCTT
CGGATATTCATTTTGAACCGCAAAAAGACAAAATGTTGATTCGATTACGTATCGACGGCGTGTTACATCCGTATAAATCA
TTTTCTCTCCAACTTGCCAATCGACTTATTTCTCGCATTAAATTATTAGCTAAACTGGATATTAGTGAATCCCGTCTTCC
GCAAGACGGGCAATTCGGTTTTAAGACAATCTTATCCGAAACATTGGATTTCCGTGTTTCGAGTTTACCGACTTATTGGG
GCGAAAAAGTCGTTTTACGGCTACAAAAAAATAAACCGACGCATTTTGATTTTACTACGTTGGGCTTCTTGGCGGAGCAG
AAGGAAAAGTTGTTGCGGGCATTGCGGCAGCCTCAAGGATTAATATTAGTTACGGGACCGACCGGCAGTGGTAAAAGTAT
TACCTTATATAGTGCGTTGGATTATTTAAATGACAGTAAAAGGCATATTTTGACCGCCGAAGATCCGATAGAAATCGAAC
TTGAAGGCATAATCCAAACGCAAATAAACCCGGCGATAGATTTAGGGTTCAGCCGGCTATTAAGAACGTTTTTACGACAA
GATCCGGATGTGATTATGTTAGGTGAAATTCGTGATCAAGAAAGTGCCGAAATTGCATTAAGTGCGGCACAAACCGGGCA
TTTAGTGCTTTCGACCTTACATACCAATGATGCGCCTTCGGCAATCGAACGCTTATTGCAGCTCGGTATCAAAGAATATG
AAATCAATAATTCGTTATTACTTGTCGTGGCGCAACGTTTAGTTCGTAAATTATGTACCGAATGCGGCGGAAAAGGCTGT
AAATATTGTTATCACGGCTACAGAGGCAGAATCGGCGTTTATCAACTGTTTTCAAAAAATGGAAAAATTTTTGAGAAATC
GACCGCTTGTTTGGATTTTGAAAACTTATATCAAAGTGCGCAACAGAAATTCAAGACGGGAATCACTTCATTAGAAGAAA
TCGAGAGAGTGTTAGGTCATGTTGAAAGTGTATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(28-92)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

67.974

98.922

0.672
  pilB Haemophilus influenzae Rd KW20

58.72

97.629

0.573
  pilB Haemophilus influenzae 86-028NP

58.278

97.629

0.569
  pilB Vibrio campbellii strain DS40M4

40.333

100

0.418
  pilB Legionella pneumophila strain ERS1305867

39.468

100

0.416
  pilB Vibrio parahaemolyticus RIMD 2210633

39.917

100

0.414
  pilB Acinetobacter baumannii D1279779

39.059

100

0.412
  pilB Vibrio cholerae strain A1552

39.212

100

0.407
  pilB Acinetobacter baylyi ADP1

38.525

100

0.405
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.538

100

0.369

Multiple sequence alignment