Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   D1110_RS04675 Genome accession   NZ_CP031856
Coordinates   1019847..1021241 (-) Length   464 a.a.
NCBI ID   WP_237586309.1    Uniprot ID   -
Organism   Actinobacillus pleuropneumoniae strain 16287-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1014847..1026241
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1110_RS04645 (D1110_04645) lptE 1015461..1015955 (+) 495 WP_005597385.1 LPS assembly lipoprotein LptE -
  D1110_RS04650 (D1110_04650) holA 1016064..1017089 (+) 1026 WP_237586307.1 DNA polymerase III subunit delta -
  D1110_RS04655 (D1110_04655) yacG 1017166..1017354 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  D1110_RS04660 (D1110_04660) coaE 1017336..1017974 (-) 639 WP_005601202.1 dephospho-CoA kinase -
  D1110_RS04665 (D1110_04665) - 1017996..1018634 (-) 639 WP_005604550.1 prepilin peptidase -
  D1110_RS04670 (D1110_04670) pilC 1018661..1019863 (-) 1203 WP_237586308.1 type II secretion system F family protein Machinery gene
  D1110_RS04675 (D1110_04675) pilB 1019847..1021241 (-) 1395 WP_237586309.1 GspE/PulE family protein Machinery gene
  D1110_RS04680 (D1110_04680) pilA 1021259..1021705 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  D1110_RS04685 (D1110_04685) radA 1021887..1023266 (+) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  D1110_RS04690 (D1110_04690) - 1023310..1023543 (-) 234 WP_005597399.1 YgjV family protein -
  D1110_RS04695 (D1110_04695) folA 1023670..1024158 (-) 489 WP_005597400.1 type 3 dihydrofolate reductase -
  D1110_RS04700 (D1110_04700) proB 1024245..1025336 (+) 1092 WP_005601213.1 glutamate 5-kinase -
  D1110_RS04705 (D1110_04705) - 1025349..1026125 (+) 777 WP_005597402.1 TSUP family transporter -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 53090.93 Da        Isoelectric Point: 6.5042

>NTDB_id=311943 D1110_RS04675 WP_237586309.1 1019847..1021241(-) (pilB) [Actinobacillus pleuropneumoniae strain 16287-1]
MQYSVCDVNNRQVFRISEEQWQKNYDERHILLRYLTVPLREDQQTLWLAIDDMNNLSACEIFAFLTHKRIEPVLVSSEEL
KYLLGQLMPEQQSVYDETELPYQPLAQAQHSDVNDPIIQVLDGLFKYGLKHNASDIHFEPQKDKMLIRLRIDGVLHPYKS
FSLQLANRLISRIKLLAKLDISESRLPQDGQFGFKTILSETLDFRVSSLPTYWGEKVVLRLQKNKPTHFDFTTLGFLAEQ
KEKFLRALRQPQGLILVTGPTGSGKSITLYSALDYLNDSKRHILTAEDPIEIELEGIIQTQINPAIDLGFSRLLRTFLRQ
DPDVIMLGEIRDQESAEIALSAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEINNSLLLVVAQRLVRKLCTECGGKGC
KYCYHGYRGRIGVYQLFSKNGKIFEKSTACLDFENLYQSAQQKFKTGITSLEEIERVLGHVESV

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=311943 D1110_RS04675 WP_237586309.1 1019847..1021241(-) (pilB) [Actinobacillus pleuropneumoniae strain 16287-1]
ATGCAGTATTCCGTATGTGATGTAAACAATCGGCAAGTATTCCGGATTTCCGAGGAACAGTGGCAAAAAAATTATGATGA
ACGTCATATCTTATTACGTTATCTCACCGTACCGCTTCGGGAAGACCAACAAACATTATGGTTGGCAATCGATGATATGA
ATAATCTCAGTGCTTGCGAGATTTTTGCTTTTCTTACGCATAAGCGAATTGAGCCGGTATTGGTCTCTTCCGAAGAATTG
AAATACCTGCTCGGGCAATTAATGCCGGAACAACAATCGGTATATGACGAAACGGAATTACCTTATCAACCTTTAGCACA
AGCGCAGCATTCGGATGTTAATGATCCGATTATTCAAGTGTTGGACGGCCTGTTTAAATACGGTTTAAAACATAATGCTT
CGGATATTCATTTTGAACCGCAAAAAGACAAAATGTTGATTCGATTACGTATCGACGGCGTGTTACATCCGTATAAATCA
TTTTCTCTCCAACTTGCCAATCGACTTATTTCTCGCATTAAATTATTAGCTAAACTGGATATTAGTGAATCCCGTCTTCC
GCAAGACGGGCAATTCGGTTTTAAGACAATCTTATCCGAAACATTGGATTTCCGTGTTTCGAGTTTACCGACTTATTGGG
GCGAAAAAGTCGTTTTACGGCTACAAAAAAATAAACCGACGCATTTTGATTTTACTACGTTGGGCTTCTTGGCGGAGCAG
AAGGAAAAGTTTTTGCGGGCATTGCGGCAGCCTCAAGGATTAATATTAGTTACGGGACCGACCGGCAGTGGTAAAAGTAT
TACCTTATATAGTGCGTTGGATTATTTAAATGACAGTAAAAGGCATATTTTGACCGCCGAAGATCCGATAGAAATCGAAC
TTGAAGGCATAATCCAAACGCAAATAAACCCGGCGATAGATTTAGGGTTCAGCCGGCTATTAAGAACGTTTTTACGACAA
GATCCGGATGTGATTATGTTAGGTGAAATTCGTGATCAAGAAAGTGCCGAAATTGCATTAAGTGCGGCACAAACCGGGCA
TTTAGTGCTTTCGACCTTACATACCAATGATGCGCCTTCGGCAATCGAACGCTTATTGCAGCTCGGTATCAAAGAATATG
AAATCAATAATTCGTTATTACTTGTCGTGGCGCAACGTTTAGTTCGTAAATTATGTACCGAATGCGGCGGAAAAGGCTGT
AAATATTGTTATCACGGCTACAGAGGCAGAATCGGCGTTTATCAACTGTTTTCAAAAAATGGAAAAATTTTTGAGAAATC
GACCGCTTGTTTGGATTTTGAAAACTTATATCAAAGTGCGCAACAGAAATTCAAGACGGGAATCACTTCATTAGAAGAAA
TCGAGAGAGTGTTAGGTCATGTTGAAAGTGTATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(28-92)

Type II/IV secretion system protein (T2SSE)

(129-390)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

67.974

98.922

0.672
  pilB Haemophilus influenzae Rd KW20

58.72

97.629

0.573
  pilB Haemophilus influenzae 86-028NP

58.278

97.629

0.569
  pilB Vibrio campbellii strain DS40M4

40.333

100

0.418
  pilB Legionella pneumophila strain ERS1305867

39.673

100

0.418
  pilB Acinetobacter baumannii D1279779

39.264

100

0.414
  pilB Vibrio parahaemolyticus RIMD 2210633

39.917

100

0.414
  pilB Acinetobacter baylyi ADP1

38.73

100

0.407
  pilB Vibrio cholerae strain A1552

39.212

100

0.407
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.538

100

0.369

Multiple sequence alignment