Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA   Type   Machinery gene
Locus tag   D1110_RS04680 Genome accession   NZ_CP031856
Coordinates   1021259..1021705 (-) Length   148 a.a.
NCBI ID   WP_005597397.1    Uniprot ID   B0BPG5
Organism   Actinobacillus pleuropneumoniae strain 16287-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 1016259..1026705
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1110_RS04655 (D1110_04655) yacG 1017166..1017354 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  D1110_RS04660 (D1110_04660) coaE 1017336..1017974 (-) 639 WP_005601202.1 dephospho-CoA kinase -
  D1110_RS04665 (D1110_04665) - 1017996..1018634 (-) 639 WP_005604550.1 prepilin peptidase -
  D1110_RS04670 (D1110_04670) pilC 1018661..1019863 (-) 1203 WP_237586308.1 type II secretion system F family protein Machinery gene
  D1110_RS04675 (D1110_04675) pilB 1019847..1021241 (-) 1395 WP_237586309.1 GspE/PulE family protein Machinery gene
  D1110_RS04680 (D1110_04680) pilA 1021259..1021705 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  D1110_RS04685 (D1110_04685) radA 1021887..1023266 (+) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  D1110_RS04690 (D1110_04690) - 1023310..1023543 (-) 234 WP_005597399.1 YgjV family protein -
  D1110_RS04695 (D1110_04695) folA 1023670..1024158 (-) 489 WP_005597400.1 type 3 dihydrofolate reductase -
  D1110_RS04700 (D1110_04700) proB 1024245..1025336 (+) 1092 WP_005601213.1 glutamate 5-kinase -
  D1110_RS04705 (D1110_04705) - 1025349..1026125 (+) 777 WP_005597402.1 TSUP family transporter -

Sequence


Protein


Download         Length: 148 a.a.        Molecular weight: 15980.38 Da        Isoelectric Point: 7.6257

>NTDB_id=311944 D1110_RS04680 WP_005597397.1 1021259..1021705(-) (pilA) [Actinobacillus pleuropneumoniae strain 16287-1]
MQKLSLIRPLTNAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLAASASYKTDVEICIYNTGDSKNCSGGQNGV
RKMTELRQAKYLNAITVEGGTITVTGKGNLQEYGYTMTPIHNGSTISWETKCKGEDLSLFPANFCASN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=311944 D1110_RS04680 WP_005597397.1 1021259..1021705(-) (pilA) [Actinobacillus pleuropneumoniae strain 16287-1]
ATGCAAAAACTAAGTCTTATTCGACCGCTTACTAACGCGTTTACTTTAATTGAATTGATGATCGTGATTGCGATTATTGC
CATTTTAGCTACGGTTGCAATTCCGTCATATAACAGTTATACCCAAAAAGCGGCGCTTTCGGAGCTATTGGCGGCATCGG
CTTCTTATAAAACGGATGTCGAGATCTGCATATATAACACCGGAGATTCTAAAAACTGTAGCGGCGGTCAAAACGGTGTC
AGAAAAATGACGGAGCTTAGACAGGCTAAATATTTAAATGCCATTACGGTGGAAGGCGGAACGATTACAGTAACGGGGAA
AGGGAATCTACAGGAATACGGTTATACGATGACACCGATTCATAACGGTAGCACTATTTCTTGGGAAACGAAATGTAAAG
GGGAGGACTTAAGTTTATTTCCGGCAAATTTCTGCGCATCAAATTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB B0BPG5

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

68.919

100

0.689

  pilA Haemophilus influenzae 86-028NP

55.102

99.324

0.547

  pilA Haemophilus influenzae Rd KW20

53.061

99.324

0.527


Multiple sequence alignment