Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   D0851_RS05635 Genome accession   NZ_CP031848
Coordinates   1206860..1208575 (+) Length   571 a.a.
NCBI ID   WP_117617742.1    Uniprot ID   A0A346RYX9
Organism   Marinobacter sp. Arc7-DN-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1201860..1213575
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0851_RS05620 (D0851_05620) - 1202807..1203235 (-) 429 WP_117617740.1 hypothetical protein -
  D0851_RS05625 (D0851_05625) - 1203947..1205233 (-) 1287 WP_117617741.1 O-antigen ligase family protein -
  D0851_RS05630 (D0851_05630) - 1205878..1206369 (-) 492 WP_413773546.1 pilin -
  D0851_RS05635 (D0851_05635) pilB 1206860..1208575 (+) 1716 WP_117617742.1 type IV-A pilus assembly ATPase PilB Machinery gene
  D0851_RS05640 (D0851_05640) pilC 1208578..1209798 (+) 1221 WP_117617743.1 type II secretion system F family protein Machinery gene
  D0851_RS05645 (D0851_05645) pilD 1209969..1210844 (+) 876 WP_117617744.1 prepilin peptidase Machinery gene
  D0851_RS05650 (D0851_05650) coaE 1210994..1211593 (+) 600 WP_117617745.1 dephospho-CoA kinase -
  D0851_RS05655 (D0851_05655) - 1211565..1212680 (+) 1116 WP_227539458.1 hypothetical protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62658.68 Da        Isoelectric Point: 5.0141

>NTDB_id=311843 D0851_RS05635 WP_117617742.1 1206860..1208575(+) (pilB) [Marinobacter sp. Arc7-DN-1]
MSTNKSNITLTGLARRFVDDGLLDEATAKDAFLQASQNRIPLITYLTQNSLADSSRLAFSAAMEFGVSVLDLSAFLPEMM
PEKVVDEKLIRKHNALPLYKRGNRLFIAVSDPTNIQALDEIKFNTGLSTDAILVDDAKLREAIDKYLESQDTTMGDLDDS
DLEGVETEGGEQEDDGAVTASEVDDAPIVKYVNKMLLDAIRGGASDVHFEPYEKIYRVRYRTDGILKEVSHPSIKLAPKI
SARVKIMAQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAKLGIDVLGYEEDQKKLYMDALA
QPQGMILVTGPTGSGKTVSLYTGLNILNTPGINISTAEDPAEINLEGINQVNVNTRVGLGFAEALRAFLRQDPDVIMVGE
IRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPSFNIATSVSLIIAQRLGRRLCNSCKQAADIPKDVLLAE
GFTQEQIDTGFTLYRPKGCDKCNGGYKGRVGIYEVVKITDELANMIMEEASSIKIAKQAQAEGFRTLRQSALRKVVEGVT
SLEEANRVTKD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=311843 D0851_RS05635 WP_117617742.1 1206860..1208575(+) (pilB) [Marinobacter sp. Arc7-DN-1]
ATGTCTACCAACAAAAGCAATATCACGCTTACCGGCCTTGCGAGGCGCTTCGTGGACGACGGGCTCCTTGACGAGGCAAC
GGCAAAAGACGCTTTTCTCCAGGCGTCTCAGAACCGGATTCCTTTAATCACCTACCTCACCCAGAACAGTCTGGCAGACA
GCTCCAGGCTTGCATTTTCAGCAGCTATGGAATTCGGGGTTTCGGTTCTGGATCTTTCCGCCTTCCTGCCGGAAATGATG
CCCGAGAAGGTGGTAGACGAGAAACTGATCCGAAAGCACAACGCTCTCCCACTCTACAAACGCGGGAATCGTTTGTTCAT
TGCGGTTTCAGACCCGACGAACATCCAGGCGCTGGATGAAATCAAGTTCAACACAGGCTTGAGCACTGACGCCATTCTGG
TCGACGACGCCAAACTGAGAGAGGCCATCGACAAGTATCTGGAATCCCAGGACACCACGATGGGAGACCTGGACGACTCG
GACCTTGAAGGCGTTGAAACCGAAGGCGGCGAGCAGGAAGACGATGGCGCGGTAACCGCAAGCGAAGTTGATGATGCGCC
CATTGTTAAGTACGTGAACAAGATGCTGCTCGATGCCATTCGTGGTGGCGCCTCGGATGTTCACTTCGAGCCTTACGAGA
AGATCTATCGTGTGCGCTACCGCACCGACGGCATTCTGAAAGAGGTCTCCCATCCTTCCATCAAGCTGGCACCCAAAATT
TCGGCCCGCGTAAAGATCATGGCCCAGTTGGACATTTCCGAGCGCCGGGTGCCTCAGGACGGCCGGATCAAGATGAAGCT
GTCGAAAACCAAGGCGATCGATTTCCGGGTGAACACACTGCCCACCCTCTGGGGCGAGAAAATCGTTTTGCGGATTCTGG
ATCCGAGCCAGGCCAAGCTCGGCATTGATGTGCTTGGGTATGAGGAAGACCAGAAAAAACTGTACATGGATGCCCTTGCT
CAGCCCCAGGGTATGATTCTGGTCACCGGCCCGACCGGGTCCGGTAAGACGGTTTCCCTCTATACCGGGCTGAACATTCT
GAACACGCCGGGTATCAATATTTCCACGGCGGAAGACCCGGCGGAAATCAACCTTGAGGGCATCAACCAGGTAAACGTGA
ACACCAGGGTGGGCCTTGGCTTCGCCGAGGCCCTGCGAGCCTTCCTGCGCCAGGACCCGGATGTGATCATGGTGGGTGAG
ATCCGGGATCTGGAAACCGCAAACATTGCCATCAAGGCGGCACAAACCGGCCACCTTGTTCTTTCAACACTCCACACCAA
CAGCGCGGCGGAAACCCTCACGCGGATGATGAACATGGGTGTACCCTCGTTCAACATTGCCACGTCGGTTAGCTTGATTA
TTGCCCAGCGCCTGGGCCGGAGGCTGTGTAATTCCTGCAAACAGGCTGCGGACATTCCGAAAGACGTGCTTTTGGCGGAA
GGGTTCACACAGGAACAAATTGATACAGGCTTCACGTTGTACCGCCCCAAGGGCTGTGATAAATGCAATGGAGGATATAA
AGGCCGCGTCGGTATTTACGAGGTGGTCAAGATTACCGACGAGCTGGCGAATATGATTATGGAAGAAGCCAGCTCCATCA
AAATTGCAAAGCAGGCTCAGGCAGAGGGCTTCCGGACATTGCGGCAATCCGCTCTTCGCAAAGTTGTTGAGGGGGTTACC
AGTCTTGAGGAAGCCAACCGCGTGACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A346RYX9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.894

100

0.571
  pilB Acinetobacter baylyi ADP1

56.119

100

0.562
  pilB Legionella pneumophila strain ERS1305867

54.417

99.124

0.539
  pilB Vibrio parahaemolyticus RIMD 2210633

51.444

97.023

0.499
  pilB Vibrio campbellii strain DS40M4

50.355

98.774

0.497
  pilB Vibrio cholerae strain A1552

54.423

91.068

0.496
  pilF Neisseria gonorrhoeae MS11

47.527

99.124

0.471
  pilB/pilB1 Synechocystis sp. PCC 6803

41.04

90.893

0.373
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.544

92.119

0.364

Multiple sequence alignment