Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   D0851_RS05645 Genome accession   NZ_CP031848
Coordinates   1209969..1210844 (+) Length   291 a.a.
NCBI ID   WP_117617744.1    Uniprot ID   A0A346RYY1
Organism   Marinobacter sp. Arc7-DN-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1204969..1215844
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0851_RS05630 (D0851_05630) - 1205878..1206369 (-) 492 WP_413773546.1 pilin -
  D0851_RS05635 (D0851_05635) pilB 1206860..1208575 (+) 1716 WP_117617742.1 type IV-A pilus assembly ATPase PilB Machinery gene
  D0851_RS05640 (D0851_05640) pilC 1208578..1209798 (+) 1221 WP_117617743.1 type II secretion system F family protein Machinery gene
  D0851_RS05645 (D0851_05645) pilD 1209969..1210844 (+) 876 WP_117617744.1 prepilin peptidase Machinery gene
  D0851_RS05650 (D0851_05650) coaE 1210994..1211593 (+) 600 WP_117617745.1 dephospho-CoA kinase -
  D0851_RS05655 (D0851_05655) - 1211565..1212680 (+) 1116 WP_227539458.1 hypothetical protein -
  D0851_RS05660 (D0851_05660) tsaA 1212887..1213633 (+) 747 WP_117617747.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32302.36 Da        Isoelectric Point: 7.5408

>NTDB_id=311845 D0851_RS05645 WP_117617744.1 1209969..1210844(+) (pilD) [Marinobacter sp. Arc7-DN-1]
MLTLDTFLSTPWLLYLTVTLVSLCIGSFLNVVILRLPKMMHQDWRCQCEEFLELPEKQRKQDERITLSRPASTCPSCGHG
IRAWENIPVVSYLVLRGRCASCKARISPRYPLIETVTAVFSVVTVALLGANESALWALLLVWALVALTVIDFDTQLLPDN
ITLPLMWLGLVLNYFGVLTDFTSAFWGAVAGYLSLWSVYWLFKLVTGKEGMGHGDFKLLAALGAWLGWQLLPAVILLSSV
VGAVVGISLMVFKKHGREVPIPFGPYLATAGLLCLWFGSEIQGFWFGFLGV

Nucleotide


Download         Length: 876 bp        

>NTDB_id=311845 D0851_RS05645 WP_117617744.1 1209969..1210844(+) (pilD) [Marinobacter sp. Arc7-DN-1]
ATGCTTACTTTGGATACCTTTCTTTCTACCCCCTGGCTGCTTTACCTTACCGTTACCCTGGTTTCCCTCTGCATCGGCAG
CTTCCTGAATGTTGTGATTCTGAGGCTGCCGAAGATGATGCACCAGGACTGGCGCTGCCAGTGCGAGGAGTTTCTGGAGT
TGCCTGAGAAGCAGCGCAAGCAGGACGAGCGTATAACTCTTTCCAGGCCGGCTTCCACCTGCCCCTCCTGCGGCCATGGC
ATTCGTGCCTGGGAAAACATTCCGGTGGTGAGCTATCTGGTGCTTCGGGGCAGGTGTGCCTCCTGCAAGGCTCGGATTTC
GCCCCGCTATCCGCTGATTGAGACGGTAACCGCGGTGTTTTCCGTGGTTACGGTCGCCCTGCTTGGGGCCAATGAGTCTG
CGCTTTGGGCGTTGTTGCTGGTGTGGGCTTTGGTGGCCCTTACCGTGATTGATTTCGATACCCAGCTGCTGCCGGATAAC
ATCACCCTGCCCCTGATGTGGCTGGGGCTGGTGCTGAATTATTTTGGTGTGCTGACGGACTTTACCAGTGCCTTCTGGGG
CGCGGTGGCTGGCTATCTTTCCCTGTGGTCGGTTTACTGGCTGTTCAAGCTGGTGACCGGGAAGGAAGGCATGGGGCATG
GCGACTTCAAGTTGCTGGCCGCTCTGGGTGCCTGGCTGGGGTGGCAGCTGTTGCCGGCGGTGATTCTGCTTTCCTCGGTG
GTTGGTGCGGTTGTGGGTATCAGCCTGATGGTGTTCAAGAAGCATGGGCGGGAAGTGCCGATTCCGTTCGGGCCTTATCT
GGCTACGGCGGGGTTGCTTTGCCTTTGGTTTGGTAGTGAGATTCAGGGGTTCTGGTTTGGGTTTTTGGGGGTTTGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(138-247)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-125)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A346RYY1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

54.015

94.158

0.509
  pilD Vibrio cholerae strain A1552

51.579

97.938

0.505
  pilD Acinetobacter nosocomialis M2

53.285

94.158

0.502
  pilD Vibrio campbellii strain DS40M4

51.228

97.938

0.502
  pilD Neisseria gonorrhoeae MS11

50

86.598

0.433

Multiple sequence alignment