Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CJA_RS13180 Genome accession   NC_010995
Coordinates   3210370..3212091 (+) Length   573 a.a.
NCBI ID   WP_012488324.1    Uniprot ID   B3PBG3
Organism   Cellvibrio japonicus Ueda107     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3194456..3213384 3210370..3212091 within 0


Gene organization within MGE regions


Location: 3194456..3213384
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJA_RS13125 (CJA_2719) - 3194507..3195325 (+) 819 WP_012488313.1 ABC transporter permease -
  CJA_RS13130 (CJA_2720) - 3195398..3196171 (+) 774 WP_012488314.1 ABC transporter ATP-binding protein -
  CJA_RS13135 (CJA_2721) - 3196202..3196939 (+) 738 WP_012488315.1 urea amidolyase associated protein UAAP1 -
  CJA_RS13140 (CJA_2722) - 3196957..3197598 (+) 642 WP_012488316.1 urea amidolyase associated protein UAAP2 -
  CJA_RS13145 (CJA_2723) uca 3197686..3201303 (+) 3618 WP_012488317.1 urea carboxylase -
  CJA_RS13150 (CJA_2724) - 3201370..3203835 (-) 2466 WP_012488318.1 DUF1631 domain-containing protein -
  CJA_RS13155 (CJA_2725) nadC 3204037..3204921 (+) 885 WP_012488319.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CJA_RS13160 (CJA_2726) - 3204955..3207981 (-) 3027 WP_012488320.1 fused MFS/spermidine synthase -
  CJA_RS13165 (CJA_2727) tfpZ 3208083..3208826 (-) 744 WP_012488321.1 TfpX/TfpZ family type IV pilin accessory protein -
  CJA_RS19890 (CJA_2728) - 3208852..3209337 (-) 486 WP_012488322.1 pilin -
  CJA_RS20025 - 3209443..3209760 (-) 318 WP_238526867.1 pilin -
  CJA_RS20030 - 3209835..3209943 (-) 109 Protein_2594 pilin -
  CJA_RS13180 (CJA_2730) pilB 3210370..3212091 (+) 1722 WP_012488324.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CJA_RS13185 (CJA_2731) pilC 3212098..3213384 (+) 1287 WP_012488325.1 type II secretion system F family protein Machinery gene

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62727.88 Da        Isoelectric Point: 5.7534

>NTDB_id=31128 CJA_RS13180 WP_012488324.1 3210370..3212091(+) (pilB) [Cellvibrio japonicus Ueda107]
MNTPVSSLSGLARRLVSDNLLDPDLAGKAIIQAKKDKVPFVHHLVSNGILDARIIARVAAEEFGTPVFDLGALNKDTIPQ
KLVDEKLIRKHQTLPLSRRGNRLFLAVADPTDLHALDEIKFNTGINTEAVLVEADKLKSTIEKYLSSQEEPLSLGDGLDS
DLEDLDVETIDTNASTGDESTEADEAPIVKYINKVLLDAIKGGSSDIHFEPYEKSYRVRFRTDGVLHEVSRPPVNLATRM
AARLKVMSQMDISERRVPQDGRIKLKVSKTRAIDFRVNTLPTLFGEKIVLRILDPSSAKLGIDALGYEDNQKKLYLDALA
QSQGMILVTGPTGSGKTVSLYTGLNILNTAEVNISTAEDPVEINLEGINQVQMNTRVGLTFAEALRSFLRQDPDVIMVGE
IRDLETAEIAIKASQTGHLVLSTLHTNSAPETLTRLLNMGVPAFNVATSVSLIIAQRLARRLCPTCRKPATDIPEEILKQ
EGFDDIGIPRAEFQLFHPVGCDKCNGGYKGRVGVYEVVRITPEIANLIMEGGNSLQIARVAKEAGFNNLRVSALRKVAMG
LTSLEEANRVTKD

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=31128 CJA_RS13180 WP_012488324.1 3210370..3212091(+) (pilB) [Cellvibrio japonicus Ueda107]
ATGAACACCCCGGTATCCTCCCTCAGCGGTTTAGCAAGGCGCTTGGTTTCCGATAACCTGCTTGATCCTGATCTTGCCGG
TAAAGCGATCATCCAAGCCAAGAAAGATAAGGTTCCTTTTGTTCACCATCTAGTGAGCAATGGCATTCTGGATGCCCGGA
TAATTGCAAGAGTAGCCGCGGAAGAGTTTGGGACACCGGTATTTGATTTAGGGGCACTCAATAAAGACACCATCCCACAA
AAGCTGGTTGATGAAAAACTTATACGTAAACACCAAACCTTGCCTTTATCCCGGCGAGGCAACCGATTGTTTCTGGCAGT
TGCCGACCCTACAGACCTGCATGCACTGGACGAAATCAAATTCAATACCGGTATCAACACCGAGGCCGTTCTGGTTGAGG
CCGATAAGCTCAAAAGTACTATTGAAAAATACCTAAGTTCCCAGGAAGAACCCCTGAGCCTGGGTGATGGTTTGGACTCT
GACCTGGAAGATTTGGATGTTGAGACCATCGACACCAACGCATCCACCGGGGATGAATCTACCGAAGCCGATGAAGCCCC
TATCGTTAAATACATTAATAAGGTACTACTGGATGCCATCAAGGGTGGCTCTTCGGATATTCACTTTGAACCCTACGAGA
AGAGTTATCGGGTTCGCTTCCGTACCGATGGTGTACTCCACGAGGTATCACGCCCACCTGTAAACCTGGCAACACGGATG
GCCGCACGCCTGAAGGTCATGTCGCAAATGGACATTTCCGAGCGTCGGGTGCCACAGGACGGACGCATTAAGCTGAAAGT
ATCGAAAACCCGGGCTATCGATTTCCGGGTTAACACCCTGCCGACCCTGTTTGGGGAAAAGATTGTACTGCGTATCCTCG
ACCCCAGTTCTGCCAAACTGGGCATTGATGCACTGGGCTATGAGGATAATCAGAAGAAACTGTACCTGGATGCCCTCGCC
CAATCGCAAGGTATGATCCTGGTTACCGGCCCGACCGGTAGTGGTAAAACGGTATCCCTTTACACGGGTCTCAACATCCT
GAACACTGCGGAGGTCAATATCTCCACTGCTGAGGACCCGGTAGAAATCAACCTGGAGGGTATTAACCAGGTACAGATGA
ATACCCGGGTGGGACTGACCTTTGCCGAAGCCCTGCGCTCCTTCCTGCGCCAGGACCCGGATGTAATCATGGTGGGTGAG
ATCCGTGACCTGGAAACCGCAGAAATCGCCATTAAAGCCTCCCAGACCGGTCACCTTGTCCTCTCCACACTCCATACCAA
CTCTGCCCCCGAAACCCTGACCCGCCTGCTTAACATGGGCGTACCGGCCTTTAACGTGGCTACCTCCGTCAGCCTGATTA
TTGCCCAACGCCTGGCACGGCGCCTGTGCCCGACATGCCGCAAACCCGCTACCGATATCCCCGAAGAGATCTTGAAGCAA
GAGGGATTTGACGACATAGGGATACCAAGAGCAGAATTCCAGCTTTTCCATCCCGTGGGTTGTGACAAGTGTAACGGCGG
CTATAAAGGCCGGGTGGGGGTTTATGAAGTGGTTCGCATCACCCCGGAGATTGCCAATCTTATAATGGAGGGCGGCAACT
CGTTGCAAATCGCCCGTGTCGCCAAAGAAGCCGGCTTTAATAATTTAAGAGTCTCAGCCCTGCGCAAAGTCGCGATGGGA
CTAACCAGCCTCGAAGAGGCCAATCGCGTAACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B3PBG3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.035

99.127

0.595
  pilB Acinetobacter baylyi ADP1

59.436

98.953

0.588
  pilB Legionella pneumophila strain ERS1305867

54.85

98.953

0.543
  pilB Vibrio parahaemolyticus RIMD 2210633

52.015

95.288

0.496
  pilB Vibrio campbellii strain DS40M4

51.554

95.462

0.492
  pilB Vibrio cholerae strain A1552

51.661

94.59

0.489
  pilF Neisseria gonorrhoeae MS11

48.063

99.127

0.476
  pilB/pilB1 Synechocystis sp. PCC 6803

42.004

90.576

0.38

Multiple sequence alignment