Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   D0511_RS04540 Genome accession   NZ_CP031761
Coordinates   1007848..1009074 (-) Length   408 a.a.
NCBI ID   WP_088531398.1    Uniprot ID   A0AAD0W2P2
Organism   Pseudoalteromonas piscicida strain DE2-A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1002848..1014074
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0511_RS04525 (D0511_04525) pilB 1004410..1006086 (-) 1677 WP_117332834.1 type IV-A pilus assembly ATPase PilB Machinery gene
  D0511_RS04530 (D0511_04530) coaE 1006196..1006825 (-) 630 WP_088531400.1 dephospho-CoA kinase -
  D0511_RS04535 (D0511_04535) pilD 1006835..1007713 (-) 879 WP_088531399.1 prepilin peptidase Machinery gene
  D0511_RS04540 (D0511_04540) pilC 1007848..1009074 (-) 1227 WP_088531398.1 type II secretion system F family protein Machinery gene
  D0511_RS23785 - 1009354..1010115 (-) 762 WP_151208242.1 hypothetical protein -
  D0511_RS04550 (D0511_04550) - 1010127..1010600 (-) 474 WP_088531396.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  D0511_RS04555 (D0511_04555) nadC 1011256..1012098 (-) 843 WP_088532285.1 carboxylating nicotinate-nucleotide diphosphorylase -
  D0511_RS04560 (D0511_04560) ampD 1012533..1013075 (+) 543 WP_088531395.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  D0511_RS04565 (D0511_04565) ampE 1013082..1013933 (+) 852 WP_088531394.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 44768.80 Da        Isoelectric Point: 9.8090

>NTDB_id=311041 D0511_RS04540 WP_088531398.1 1007848..1009074(-) (pilC) [Pseudoalteromonas piscicida strain DE2-A]
MSKDKNRDHELFIWFGVNTRGKKLNGEMTGQSVALVKAQLRKQGITPSKVKRKPKPLFGFSGTQKIKSVDIATTTRQIAT
MLMAGVSLVQTLDMLARGIKNKSLSKLIAHIADEVKAGQPLAKSLRAYPRYFDELYCDLVHSGEQSGALDTIFDRIALYK
EKTEALKSKIKKALFYPIAVVAVALIVTGILLIFVVPQFQDIFAGFGAELPAFTLMVIAISEFMQEYWWVFVLGLGLFGY
TYKEMLIRSAAVKHFNDRMILRIPVIGEILKKAAVARYARTLSTTFAAGVPLGDALDSAAGASGNIIYKNAIKEIKAEVN
SGNQMHWAMHNTKVFPDMVIQMVSIGEESGSLDGMLAKVANIYEQEVDDAVDGLSSLLEPLIMVVLGVLIGGLIIAMYLP
IFQLGSVI

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=311041 D0511_RS04540 WP_088531398.1 1007848..1009074(-) (pilC) [Pseudoalteromonas piscicida strain DE2-A]
ATGAGCAAAGACAAGAATAGAGACCATGAGCTTTTTATTTGGTTTGGAGTAAATACTCGAGGCAAAAAACTTAATGGTGA
GATGACAGGCCAAAGTGTGGCCCTAGTCAAAGCCCAGCTGAGAAAACAAGGCATTACCCCATCTAAAGTTAAACGCAAGC
CTAAACCTCTCTTTGGCTTTTCTGGTACTCAAAAAATTAAATCTGTTGATATAGCAACGACAACCCGACAAATTGCTACA
ATGTTAATGGCAGGTGTTTCTTTAGTACAAACATTAGATATGCTTGCTCGTGGCATCAAAAATAAAAGCTTATCTAAGCT
GATTGCCCATATCGCTGATGAAGTGAAAGCAGGCCAGCCTCTTGCTAAATCTCTAAGAGCATACCCTCGATATTTTGATG
AGCTCTATTGTGACCTTGTCCATTCAGGGGAACAGTCTGGTGCTCTCGATACCATATTTGACCGCATCGCGCTTTATAAG
GAGAAGACTGAAGCACTTAAGTCTAAGATTAAAAAGGCGCTGTTCTACCCTATTGCCGTAGTGGCTGTAGCACTCATAGT
TACTGGTATTTTGCTTATTTTCGTTGTACCTCAATTCCAAGATATTTTTGCTGGTTTTGGCGCCGAACTCCCAGCCTTTA
CGCTAATGGTTATCGCGATTTCCGAATTTATGCAGGAATATTGGTGGGTATTTGTGCTTGGACTTGGCCTTTTTGGTTAT
ACCTATAAAGAAATGCTTATTCGCAGCGCTGCGGTAAAGCACTTTAATGACCGTATGATTTTACGTATTCCAGTGATTGG
CGAGATCCTTAAAAAAGCAGCAGTGGCGCGTTATGCTCGTACGCTTTCGACCACCTTTGCAGCGGGTGTGCCCCTTGGCG
ATGCGCTAGATTCTGCCGCAGGAGCGTCGGGCAATATTATTTATAAAAATGCAATTAAAGAAATTAAAGCAGAGGTTAAC
TCGGGTAACCAAATGCACTGGGCAATGCATAACACCAAAGTCTTCCCTGATATGGTGATACAAATGGTGTCTATTGGTGA
AGAATCGGGCTCTCTAGATGGCATGCTGGCAAAGGTTGCTAACATCTATGAGCAAGAGGTCGATGATGCGGTGGATGGAC
TATCTAGTCTATTAGAGCCTCTCATTATGGTGGTACTCGGCGTACTGATCGGTGGCCTAATTATTGCCATGTACTTGCCG
ATATTCCAATTAGGCTCAGTTATTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(75-197)

Type II secretion system (T2SS), protein F (T2SSF)

(279-400)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

59.25

98.039

0.581
  pilC Legionella pneumophila strain ERS1305867

54.68

99.51

0.544
  pilC Acinetobacter baylyi ADP1

50.735

100

0.507
  pilC Acinetobacter baumannii D1279779

51.637

97.304

0.502
  pilC Vibrio cholerae strain A1552

44.862

97.794

0.439
  pilC Vibrio campbellii strain DS40M4

44.059

99.02

0.436
  pilG Neisseria gonorrhoeae MS11

42.928

98.775

0.424
  pilG Neisseria meningitidis 44/76-A

42.928

98.775

0.424

Multiple sequence alignment