Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   D0N37_RS14480 Genome accession   NZ_CP031759
Coordinates   3105943..3107619 (+) Length   558 a.a.
NCBI ID   WP_117332834.1    Uniprot ID   A0AAD0RF77
Organism   Pseudoalteromonas piscicida strain DE1-A     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3100943..3112619
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0N37_RS14455 (D0N37_14455) nadC 3100961..3101803 (+) 843 WP_119232292.1 carboxylating nicotinate-nucleotide diphosphorylase -
  D0N37_RS25640 (D0N37_14460) - 3102459..3102908 (+) 450 WP_119231796.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  D0N37_RS14465 (D0N37_14465) pilC 3102959..3104182 (+) 1224 WP_119231797.1 type II secretion system F family protein Machinery gene
  D0N37_RS14470 (D0N37_14470) pilD 3104316..3105194 (+) 879 WP_088531399.1 prepilin peptidase Machinery gene
  D0N37_RS14475 (D0N37_14475) coaE 3105204..3105833 (+) 630 WP_119231798.1 dephospho-CoA kinase -
  D0N37_RS14480 (D0N37_14480) pilB 3105943..3107619 (+) 1677 WP_117332834.1 type IV-A pilus assembly ATPase PilB Machinery gene
  D0N37_RS14485 (D0N37_14485) katG 3108208..3110391 (-) 2184 WP_119231799.1 catalase/peroxidase HPI -
  D0N37_RS14490 (D0N37_14490) nhaA 3110588..3111987 (-) 1400 Protein_2803 Na+/H+ antiporter NhaA -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62071.36 Da        Isoelectric Point: 5.8452

>NTDB_id=311024 D0N37_RS14480 WP_117332834.1 3105943..3107619(+) (pilB) [Pseudoalteromonas piscicida strain DE1-A]
MEHHSPLLRKFITLGRVTAEQIKSRQSEANTTAELICLSSGMSSKELAEECLDLFKVPFFNLDHFNVSEIPSALVKEKLI
RKHHILPLVQKGRKLYVAASDPTDFGAFENFEFSTGLQCEVLVTDYKKLEAKIDQLFDATGGLSISEEEFKEFADLEVDD
EPKQQNTNEDKDDAPIIVYINKILMDAIKKGASDLHFEPYEKKYRVRFRIDGLLHEMASPPNTLATRLAARIKVMAHLDI
AEKRKPQDGRIKLKISERKSIDFRVSTLPTMWGEKIVMRILDSSSAMLGIDVLGYEPEQKKLYLDALEQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGINQVQINIKADMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETLTRLLNMGVPAYNVASSVSLIIAQRLARRLCPKCKTAETLPEEALLGQGFTAEQIKEITL
FAPKGCEHCTDGYKGRVGIYEVVKITPELSHLIMEGGNSLEIAEKAEQLGFDNLRKSGLRKAAAGVTSLTEINRVTSY

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=311024 D0N37_RS14480 WP_117332834.1 3105943..3107619(+) (pilB) [Pseudoalteromonas piscicida strain DE1-A]
ATGGAACATCATTCCCCGCTACTGCGTAAATTTATTACCTTAGGGCGAGTCACTGCTGAGCAAATAAAGTCTCGCCAGTC
AGAAGCAAACACCACTGCCGAGTTGATTTGCCTAAGTTCAGGAATGAGCTCCAAAGAGCTAGCTGAAGAATGCCTAGACT
TATTTAAAGTGCCATTCTTTAATCTTGATCACTTTAATGTCAGTGAAATTCCATCAGCGCTAGTCAAAGAAAAACTGATC
CGTAAACACCATATTTTACCGCTAGTACAAAAAGGTAGAAAACTCTACGTTGCTGCCTCCGATCCCACTGATTTTGGCGC
ATTTGAAAACTTTGAGTTTAGTACTGGGTTACAGTGTGAAGTACTGGTAACCGACTACAAAAAACTCGAAGCAAAGATTG
ATCAGCTGTTTGATGCAACAGGCGGCCTCTCGATCTCTGAAGAAGAATTTAAAGAATTTGCAGACTTAGAAGTCGATGAC
GAGCCTAAACAGCAAAACACCAACGAAGACAAAGACGATGCGCCAATCATCGTTTATATCAATAAAATCTTAATGGACGC
GATAAAAAAAGGCGCGTCGGATCTGCACTTTGAGCCCTACGAGAAAAAATATCGCGTTCGCTTTCGTATCGATGGCCTGC
TGCATGAAATGGCAAGCCCACCTAACACACTAGCGACTCGGCTCGCTGCCCGTATCAAAGTAATGGCGCACTTAGACATA
GCGGAAAAACGTAAACCGCAAGATGGCCGCATTAAATTAAAGATTTCCGAGCGCAAAAGTATCGATTTTCGTGTGAGTAC
GCTGCCTACCATGTGGGGCGAGAAAATCGTAATGCGTATTCTAGACTCGTCCAGCGCCATGCTCGGCATCGATGTGCTAG
GTTATGAGCCAGAGCAGAAAAAACTCTACCTCGACGCACTAGAGCAGCCTCAGGGCATGATTTTGGTAACCGGCCCAACG
GGTTCTGGTAAAACTGTTTCACTTTACACGGGTTTAAATATTCTTAATCAGCCAGAGCGCAATATCAGCACAGCGGAAGA
CCCAGTCGAAATAAACCTCGAGGGCATTAACCAAGTACAAATCAATATCAAAGCGGATATGACCTTTGCCAATGCCTTGA
GGGCATTCCTGCGTCAGGATCCTGATGTGGTGATGGTAGGTGAGATCCGTGACCTTGAAACCGCAGAGATCTCAATAAAA
GCGGCTCAAACCGGTCACTTAGTATTGAGTACATTGCATACCAATTCTGCGCCTGAAACGCTCACCCGTCTATTAAACAT
GGGCGTGCCGGCGTACAACGTAGCAAGCTCAGTGAGTCTGATTATTGCCCAGCGTCTCGCAAGACGCCTCTGTCCTAAGT
GTAAAACAGCAGAAACGCTGCCGGAAGAAGCATTGCTAGGGCAAGGCTTTACTGCCGAGCAGATCAAAGAGATTACCCTT
TTTGCGCCTAAAGGCTGTGAACACTGCACCGATGGTTACAAGGGCCGTGTGGGTATTTATGAAGTGGTAAAAATAACGCC
AGAGCTTTCGCACCTGATCATGGAAGGAGGCAATTCTCTTGAGATTGCCGAAAAAGCAGAGCAGTTAGGTTTTGATAACT
TACGTAAGTCTGGTTTGAGAAAAGCCGCGGCAGGCGTAACATCGCTCACAGAAATCAACCGAGTGACAAGTTATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(182-449)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.544

100

0.588
  pilB Acinetobacter baylyi ADP1

56.915

100

0.575
  pilB Vibrio cholerae strain A1552

53.273

98.566

0.525
  pilB Legionella pneumophila strain ERS1305867

54.894

93.369

0.513
  pilB Vibrio parahaemolyticus RIMD 2210633

53.409

94.624

0.505
  pilB Vibrio campbellii strain DS40M4

52.93

94.803

0.502
  pilF Neisseria gonorrhoeae MS11

47.312

100

0.473
  pilB/pilB1 Synechocystis sp. PCC 6803

35.91

100

0.4
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.048

94.086

0.367

Multiple sequence alignment