Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   D0N37_RS14470 Genome accession   NZ_CP031759
Coordinates   3104316..3105194 (+) Length   292 a.a.
NCBI ID   WP_088531399.1    Uniprot ID   A0AAD0W2V7
Organism   Pseudoalteromonas piscicida strain DE1-A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3099316..3110194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0N37_RS14450 (D0N37_14450) ampD 3099843..3100385 (-) 543 WP_119231795.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  D0N37_RS14455 (D0N37_14455) nadC 3100961..3101803 (+) 843 WP_119232292.1 carboxylating nicotinate-nucleotide diphosphorylase -
  D0N37_RS25640 (D0N37_14460) - 3102459..3102908 (+) 450 WP_119231796.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  D0N37_RS14465 (D0N37_14465) pilC 3102959..3104182 (+) 1224 WP_119231797.1 type II secretion system F family protein Machinery gene
  D0N37_RS14470 (D0N37_14470) pilD 3104316..3105194 (+) 879 WP_088531399.1 prepilin peptidase Machinery gene
  D0N37_RS14475 (D0N37_14475) coaE 3105204..3105833 (+) 630 WP_119231798.1 dephospho-CoA kinase -
  D0N37_RS14480 (D0N37_14480) pilB 3105943..3107619 (+) 1677 WP_117332834.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 292 a.a.        Molecular weight: 32489.80 Da        Isoelectric Point: 6.6755

>NTDB_id=311023 D0N37_RS14470 WP_088531399.1 3104316..3105194(+) (pilD) [Pseudoalteromonas piscicida strain DE1-A]
MQDIINLMQTQLWYWLLTVGLVSLCIGSFLNVVILRLPKMMKQSWQSECRVLLADELPKQTAEAPVFNLIKPNSHCPSCK
TPIKAWQNIPLISWLLLKGKCNHCDASISVRYPLVELTTSLLSTWVAWHFGATLEATMYIVITWVLVALTVIDIDEMLLP
DQLTLPTLWLVLVASCFGVGIDPNSAIIGAAVGYLSLWSVYWLFKLLTGKEGMGYGDFKLLAIFGALMGWQAILTIVLLS
SLVGAIIGSIQLSVQGRDKATPIPFGPYLAIAGWITLMYGEQLQLWYVNLIM

Nucleotide


Download         Length: 879 bp        

>NTDB_id=311023 D0N37_RS14470 WP_088531399.1 3104316..3105194(+) (pilD) [Pseudoalteromonas piscicida strain DE1-A]
ATGCAAGATATTATCAACTTGATGCAAACCCAGTTATGGTATTGGTTACTCACCGTGGGACTCGTTAGTCTTTGCATCGG
CAGCTTTCTCAATGTGGTTATTCTTCGCTTACCTAAAATGATGAAGCAGAGTTGGCAGTCAGAATGTCGCGTGTTGCTCG
CTGATGAGCTACCAAAACAAACCGCTGAAGCACCTGTTTTTAATTTAATTAAGCCAAACTCTCATTGCCCCAGCTGCAAA
ACGCCGATTAAGGCGTGGCAAAACATTCCACTGATCAGTTGGCTATTATTAAAAGGCAAATGCAACCACTGCGACGCGTC
AATCTCTGTCCGCTATCCTTTGGTAGAGCTAACAACCTCCCTACTCTCCACATGGGTTGCGTGGCATTTTGGTGCCACTT
TAGAAGCCACGATGTATATTGTGATTACTTGGGTATTAGTCGCACTTACGGTTATCGATATCGACGAAATGTTGCTGCCC
GATCAGTTAACGCTACCAACCTTATGGTTAGTGCTGGTTGCCAGTTGTTTTGGTGTTGGCATCGACCCCAATAGTGCCAT
TATTGGTGCAGCGGTTGGTTATTTAAGTTTATGGAGTGTGTATTGGCTCTTTAAATTACTCACAGGTAAAGAAGGCATGG
GCTACGGCGACTTTAAACTGCTGGCCATCTTTGGCGCATTAATGGGCTGGCAAGCAATCTTAACCATAGTGCTACTTTCA
AGTCTGGTCGGCGCAATCATTGGTAGCATCCAATTAAGCGTTCAAGGCCGAGATAAAGCCACGCCTATTCCGTTTGGTCC
TTACTTAGCCATTGCAGGTTGGATCACTCTAATGTATGGCGAACAGCTGCAGCTGTGGTACGTAAACCTAATAATGTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(140-248)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(22-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

53.979

98.973

0.534
  pilD Acinetobacter nosocomialis M2

53.979

98.973

0.534
  pilD Vibrio cholerae strain A1552

51.786

95.89

0.497
  pilD Vibrio campbellii strain DS40M4

49.828

99.658

0.497
  pilD Neisseria gonorrhoeae MS11

43.939

90.411

0.397

Multiple sequence alignment