Detailed information NTG0310970

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: D0Y85_RS16160
• Genome accession: NZ_CP031741
• Coordinates: 3517443..3519170 (-)
• Length: 575 a.a.
• NCBI ID: WP_117311242.1
• Uniprot ID: -
• Organism: Stenotrophomonas sp. G4
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3512443..3524170

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D0Y85_RS16145	sucC	3512658..3513827 (-)	1170	WP_025874298.1	ADP-forming succinate--CoA ligase subunit beta	-
D0Y85_RS16150	-	3514204..3515817 (+)	1614	WP_117311237.1	ATP-binding protein	-
D0Y85_RS16155	pilR	3515872..3517245 (+)	1374	WP_117311240.1	sigma-54 dependent transcriptional regulator	Regulator
D0Y85_RS16160	pilB	3517443..3519170 (-)	1728	WP_117311242.1	type IV-A pilus assembly ATPase PilB	Machinery gene
D0Y85_RS16165	-	3519392..3519802 (-)	411	WP_117311244.1	pilin	-
D0Y85_RS16170	comP	3519932..3520375 (-)	444	WP_117311246.1	pilin	Machinery gene
D0Y85_RS16175	pilC	3520724..3521983 (+)	1260	WP_117311249.1	type II secretion system F family protein	Machinery gene
D0Y85_RS16180	-	3521992..3522855 (+)	864	WP_025874291.1	A24 family peptidase	-
D0Y85_RS16185	coaE	3522867..3523478 (+)	612	WP_117311250.1	dephospho-CoA kinase	-
D0Y85_RS16190	-	3523531..3523917 (-)	387	WP_117311251.1	hypothetical protein	-

Sequence

Protein

• Length: 575 a.a.
• Molecular weight: 62287.16 Da
• Isoelectric Point: 5.7256

>NTDB_id=310970 D0Y85_RS16160 WP_117311242.1 3517443..3519170(-) (pilB) [Stenotrophomonas sp. G4]
MNALTTSNLVGITGLARRLVQDGALDEAAARDAMAKATAARQPLPTWFAQNKVVGASQLAAANAVEFGMPLFDVSTFDSS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWQSSHDTIGDALGGD
DEGMANLDIGGGDDEMGNTDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPNKL
NQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTRQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLFL
DAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDII
MVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCSNCKRPANLPEHA
LLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGLYQVMPMTDEIATIVLAGGNALQIAEAAQAIGVNDLRQSALKKAA
AGVTSLAEINRVTKD

Nucleotide

• Length: 1728 bp

>NTDB_id=310970 D0Y85_RS16160 WP_117311242.1 3517443..3519170(-) (pilB) [Stenotrophomonas sp. G4]
ATGAACGCACTCACTACTTCTAACCTCGTCGGCATCACCGGCTTGGCCCGCCGCCTGGTCCAGGACGGCGCGCTGGATGA
GGCTGCCGCCCGCGATGCCATGGCCAAGGCCACTGCCGCGCGCCAGCCGCTGCCAACCTGGTTCGCGCAGAACAAGGTGG
TCGGGGCTTCGCAGCTGGCGGCCGCCAACGCGGTTGAGTTCGGCATGCCGCTGTTCGACGTGTCTACCTTCGACAGCAGC
CAGAACGCGATGAGCCTCGTCAGCGAGGAGCTGCTGCGCAAGCACAACGTGTTGCCGCTGTTCAAACGCGGCGGCAAGCT
GTTCGTCGGCACCAGCAACCCCACCCATTCTCTGGACGAGATCAAGTTCCACACCAATCTGGTGGTGGAGCCGATCCTGG
TCGATGAAGACCAGATCCGTCGCACCCTCGAGCAGTGGCAGTCCAGCCACGACACCATTGGTGATGCCTTGGGCGGCGAT
GACGAGGGCATGGCGAACCTCGACATCGGCGGTGGCGACGATGAGATGGGCAACACCGACAGTGGCATCGACGCCAAGGG
CGACGATACCCCGGTGGTCAAGTTCGTCAACAAGGTGCTGGTCGATGCGATCCGCAAGGGCGCCTCGGACATCCATTTCG
AGCCCTACGAAGACGACTACCGCGTGCGTCTGCGCATCGACGGCCTGCTGAAGATGGTCGCGCGCGCGCCGAACAAGCTC
AACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCGCAACTGGATATCGCCGAAAAGCGCGTGCCGCAGGATGGGCGTAT
CAAGCTCAATCTTTCCAAGACCCGGCAGATCGACTTCCGCGTCAGCACGCTGCCTACGCTGTTCGGTGAGAAGGTGGTGC
TGCGTATCCTCGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGACCAGCAGAAGCTGTTCCTG
GATGCGATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGTCCGACCGGTTCGGGCAAAACGGTGTCGCTGTATACCGC
GCTGGGCATCCTCAACGACGAGACCCGCAACATTTCCACTGCCGAAGATCCGGTGGAAATCCGCCTGCCGGGCGTCAACC
AGGTGCAGCAGAACAACAAGCGCGGCATGACCTTTGCTGCAGCGCTGCGCTCGTTCCTGCGACAGGATCCGGACATCATC
ATGGTCGGCGAAATCCGCGACCTGGAAACGGCGGAGATCGCGATCAAGGCGGCGCAGACCGGTCACATGGTGCTGTCCAC
GCTGCACACCAACGACGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGTATCGCGCCGTACAACATCACCAGTTCGG
TGACGCTGGTGATCGCCCAGCGCCTGGCGCGCCGGCTGTGTTCGAACTGCAAGCGCCCGGCCAATCTACCGGAGCATGCG
CTGCTGGCCGAGGGCTTCACCCAGGCGCAGCTGGATGCAGGCATCGAACTGTATGAGGCGGTGGGCTGCGATGAGTGCAC
CGAGGGTTACAAGGGCCGTACCGGCCTCTACCAGGTGATGCCGATGACCGACGAGATCGCGACCATCGTGCTGGCGGGCG
GCAACGCGCTGCAGATTGCCGAGGCCGCGCAGGCGATCGGTGTGAATGACCTGCGGCAGTCGGCGTTGAAGAAGGCGGCT
GCGGGTGTGACCAGCCTGGCCGAGATCAACCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (50-150)

• Type II/IV secretion system protein (T2SSE) (198-465)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	55.536	100	0.558
pilB	Acinetobacter baylyi ADP1	55.19	100	0.555
pilB	Legionella pneumophila strain ERS1305867	52.113	98.783	0.515
pilB	Vibrio cholerae strain A1552	48.776	99.478	0.485
pilF	Neisseria gonorrhoeae MS11	48.944	98.783	0.483
pilB	Vibrio parahaemolyticus RIMD 2210633	46.235	99.304	0.459
pilB	Vibrio campbellii strain DS40M4	44.755	99.478	0.445