Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   D0B88_RS07790 Genome accession   NZ_CP031727
Coordinates   1841895..1843619 (-) Length   574 a.a.
NCBI ID   WP_151056321.1    Uniprot ID   -
Organism   Cellvibrio sp. KY-YJ-3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1836895..1848619
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0B88_RS07765 (D0B88_07825) - 1837880..1838836 (+) 957 WP_151056313.1 Nudix family hydrolase -
  D0B88_RS07770 (D0B88_07830) yacG 1838853..1839065 (-) 213 WP_151056315.1 DNA gyrase inhibitor YacG -
  D0B88_RS07775 (D0B88_07835) coaE 1839074..1839685 (-) 612 WP_151056317.1 dephospho-CoA kinase -
  D0B88_RS07780 (D0B88_07840) pilD 1839712..1840608 (-) 897 WP_370452492.1 A24 family peptidase Machinery gene
  D0B88_RS07785 (D0B88_07845) pilC 1840611..1841891 (-) 1281 WP_151056319.1 type II secretion system F family protein Machinery gene
  D0B88_RS07790 (D0B88_07850) pilB 1841895..1843619 (-) 1725 WP_151056321.1 type IV-A pilus assembly ATPase PilB Machinery gene
  D0B88_RS07795 (D0B88_07855) - 1843948..1844466 (+) 519 WP_151056323.1 pilin -
  D0B88_RS07800 (D0B88_07860) - 1844514..1845734 (+) 1221 WP_151056325.1 serine hydrolase -
  D0B88_RS07805 (D0B88_07865) tfpZ 1845759..1846460 (+) 702 WP_151056327.1 TfpX/TfpZ family type IV pilin accessory protein -
  D0B88_RS07810 (D0B88_07870) - 1846629..1847099 (+) 471 WP_151056329.1 pilin -
  D0B88_RS07815 (D0B88_07875) nadC 1847100..1847954 (-) 855 WP_151056331.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62702.89 Da        Isoelectric Point: 5.5835

>NTDB_id=310766 D0B88_RS07790 WP_151056321.1 1841895..1843619(-) (pilB) [Cellvibrio sp. KY-YJ-3]
MNNPTPASLNGLARRLVADNILEIELATKAVAQAKKDKMPFVQHLVANGILDARTLALVASEEFGSPVFDLNALNKDLIP
QKLVDDKLIRKHHTLPIARRGNRLFLAVTDPTDLHALDEIKFNTGINTEAVLVEADKLQVAIDKYLNAQEESLGDALGGL
DDDMNELDVQAVSDAGSPDEANEADEAPIVKYINKVLLDAIKGGSSDIHFEPYEKSYRIRFRTDGVLHEVSRPPVNLAFR
MAARLKVMSQMDISERRLPQDGRIKLKVSKTRAIDFRVNTLPTLFGEKIVLRILDPSSAKLGIDALGYEDEQKKLYMDAL
AQSQGMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLEGINQVQMNSRVGLTFAEALRSFLRQDPDVIMVG
EIRDLETAEIAIKASQTGHLVLSTLHTNSAPETLTRLLNMGVPAFNVATSVSLIIAQRLARRLCSACKKPATDIPADILT
QEGFDEIGIPRAEFQLFHPGGCEKCNNGYKGRVGVYEVVRITPTIANLIMEGGNSLQIARAAKEAGFNNLRVSALRKVAM
GLTSLEEANRVTKD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=310766 D0B88_RS07790 WP_151056321.1 1841895..1843619(-) (pilB) [Cellvibrio sp. KY-YJ-3]
ATGAATAACCCAACTCCCGCATCATTAAATGGATTAGCACGCCGGCTGGTCGCTGACAATATTTTGGAGATAGAGCTAGC
AACCAAGGCTGTCGCTCAGGCAAAAAAGGACAAAATGCCTTTCGTTCAGCATTTGGTTGCAAATGGAATTCTAGATGCAC
GAACCTTGGCATTAGTCGCCTCTGAAGAATTCGGTTCCCCGGTTTTCGATTTAAATGCGCTTAATAAGGATCTAATTCCG
CAAAAGTTAGTAGACGATAAACTGATTCGAAAACACCATACCTTACCAATTGCTCGTCGTGGAAACCGCCTGTTTCTGGC
AGTTACTGATCCCACTGATTTACATGCACTGGATGAAATTAAATTTAATACGGGGATAAATACCGAAGCGGTACTTGTTG
AAGCAGACAAACTTCAAGTTGCTATCGACAAGTACCTCAATGCTCAAGAAGAGTCACTAGGCGATGCATTGGGCGGCCTT
GATGACGATATGAATGAGCTTGATGTCCAAGCTGTTAGCGACGCAGGTTCACCGGATGAAGCCAACGAAGCCGATGAAGC
ACCGATCGTTAAATACATTAACAAAGTTTTATTGGATGCTATTAAGGGTGGCTCATCTGATATTCACTTTGAACCCTATG
AAAAAAGTTACCGCATTCGTTTCCGTACCGATGGTGTTTTACATGAGGTATCCCGCCCGCCCGTAAACCTTGCGTTTCGT
ATGGCGGCGCGCTTGAAAGTCATGTCACAAATGGATATTTCAGAGCGTCGCTTGCCGCAAGATGGCCGCATAAAATTAAA
AGTATCCAAAACCCGCGCTATCGATTTCCGAGTAAACACCCTGCCCACCTTGTTCGGCGAGAAAATTGTATTGCGTATTC
TCGATCCCAGCTCCGCAAAATTGGGGATTGATGCATTAGGTTATGAAGACGAGCAGAAAAAGCTCTATATGGATGCGCTA
GCGCAATCGCAGGGCATGATTCTGGTAACAGGCCCCACCGGTAGCGGTAAAACGGTATCGCTTTATACCGGTTTGAATAT
TTTGAATACCAGCGAAGTGAATATTTCCACTGCGGAAGACCCGGTGGAAATTAACCTTGAAGGCATTAATCAGGTTCAAA
TGAACTCACGTGTTGGGCTTACGTTTGCGGAGGCCCTACGCTCCTTCTTGCGTCAGGATCCAGATGTAATTATGGTGGGG
GAAATTCGCGACCTTGAAACCGCCGAAATTGCAATTAAAGCATCACAAACAGGTCACTTGGTCCTATCTACTCTGCACAC
CAACTCAGCGCCGGAAACCTTGACGCGCTTGCTGAATATGGGAGTACCCGCATTTAACGTTGCAACCAGTGTGAGCTTGA
TTATTGCCCAGCGGTTGGCGCGCAGACTGTGCAGTGCCTGTAAAAAACCAGCAACCGATATTCCTGCCGACATACTCACT
CAGGAAGGTTTTGATGAAATTGGGATTCCACGAGCGGAATTTCAATTGTTCCATCCGGGAGGTTGCGAGAAGTGCAATAA
CGGTTATAAAGGGCGCGTGGGGGTATATGAAGTGGTTCGCATCACCCCAACAATTGCCAACCTTATAATGGAGGGCGGCA
ATTCATTGCAAATCGCACGCGCTGCCAAAGAAGCGGGTTTTAATAATTTACGGGTATCTGCATTGCGCAAAGTCGCAATG
GGACTAACCAGCCTCGAAGAGGCCAACCGAGTCACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.14

99.652

0.599
  pilB Acinetobacter baylyi ADP1

58.537

100

0.585
  pilB Legionella pneumophila strain ERS1305867

54.211

99.303

0.538
  pilB Vibrio cholerae strain A1552

53.039

94.599

0.502
  pilB Vibrio campbellii strain DS40M4

50.088

99.129

0.497
  pilB Vibrio parahaemolyticus RIMD 2210633

51.005

95.296

0.486
  pilF Neisseria gonorrhoeae MS11

47.183

98.955

0.467
  pilB/pilB1 Synechocystis sp. PCC 6803

41.313

90.244

0.373

Multiple sequence alignment