Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BEN71_RS02140 Genome accession   NZ_CP031716
Coordinates   315470..317182 (-) Length   570 a.a.
NCBI ID   WP_068973843.1    Uniprot ID   -
Organism   Acinetobacter wuhouensis strain WCHA60     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 310470..322182
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BEN71_RS02115 (BEN71_02115) rlmB 310967..311719 (+) 753 WP_068973848.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  BEN71_RS02120 (BEN71_02120) - 311776..312693 (+) 918 WP_068973847.1 DMT family transporter -
  BEN71_RS02125 (BEN71_02125) coaE 312706..313317 (-) 612 WP_068973846.1 dephospho-CoA kinase -
  BEN71_RS02130 (BEN71_02130) pilD 313356..314216 (-) 861 WP_068973845.1 A24 family peptidase Machinery gene
  BEN71_RS02135 (BEN71_02135) pilC 314216..315442 (-) 1227 WP_068973844.1 type II secretion system F family protein Machinery gene
  BEN71_RS02140 (BEN71_02140) pilB 315470..317182 (-) 1713 WP_068973843.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BEN71_RS02145 (BEN71_02145) tpiA 317472..318266 (+) 795 WP_068973842.1 triose-phosphate isomerase -
  BEN71_RS02150 (BEN71_02150) secG 318276..318608 (+) 333 WP_068973841.1 preprotein translocase subunit SecG -
  BEN71_RS02175 (BEN71_02175) rimP 319613..320137 (+) 525 WP_068973840.1 ribosome maturation factor RimP -
  BEN71_RS02180 (BEN71_02180) nusA 320172..321656 (+) 1485 WP_068973839.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63282.68 Da        Isoelectric Point: 4.9132

>NTDB_id=310673 BEN71_RS02140 WP_068973843.1 315470..317182(-) (pilB) [Acinetobacter wuhouensis strain WCHA60]
MSIQNGSPKFSGYIRRLVDEGFLSAQQMKDAIDSAKKNKMSTVAYLVEKLDIKAILIAEMISSEFGEPIFDLDSFNPEML
PKEVADNNLCFKHKVIPLVQRGNILYVATSDPTNVEALDAIRFNCGLNIEPIIVEYDKLEKALEKHFTVEESFSFGEEDF
DVDIIATDDQESTDVDNDTPEDEAPIVKYVNKLLVDAIRMGASDLHFEPYEKSYRVRYRVDGVLRSITNPPLQLANRLAS
RLKVMSKMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEHQKALFMEALEKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLSFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSHCKAPVEIPKQSLLEMGF
TEQDLSNPDLQIYQPIGCSECREGYKGRVGVYEVMKVTPEISKIIMEDGNALQIAAASEKLGFNNLRRSGLIKVMNGLTS
LQEINRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=310673 BEN71_RS02140 WP_068973843.1 315470..317182(-) (pilB) [Acinetobacter wuhouensis strain WCHA60]
ATGTCTATACAGAATGGTTCACCTAAGTTTTCGGGATATATTCGTCGCTTAGTGGATGAAGGCTTTTTATCAGCCCAACA
AATGAAAGATGCGATTGACAGTGCTAAAAAGAATAAAATGAGCACAGTTGCCTATTTAGTCGAAAAATTAGATATTAAAG
CGATTCTCATTGCTGAAATGATCTCATCTGAATTTGGCGAACCAATTTTTGATCTAGATAGCTTTAACCCTGAAATGTTA
CCCAAAGAAGTGGCTGATAATAATTTATGTTTCAAACATAAAGTGATTCCTTTGGTACAGCGTGGCAATATTTTATATGT
TGCGACCTCAGATCCAACCAATGTTGAAGCGCTTGACGCCATTCGTTTTAATTGTGGGCTCAACATTGAACCGATCATTG
TTGAATATGACAAATTAGAAAAAGCTTTAGAAAAGCATTTTACAGTAGAAGAAAGTTTTAGTTTTGGCGAAGAAGACTTT
GATGTCGATATTATTGCCACAGATGATCAAGAATCAACGGACGTCGATAATGACACCCCAGAAGATGAAGCGCCGATTGT
TAAATATGTTAATAAGTTATTGGTCGATGCAATTCGAATGGGCGCATCCGATTTGCATTTTGAACCCTATGAAAAATCTT
ATCGTGTGCGTTACCGTGTCGATGGTGTTTTAAGATCTATTACCAACCCACCACTACAATTGGCAAATCGTTTAGCTTCA
CGTCTAAAAGTGATGTCCAAAATGGATATTTCTGAAAAACGTGTTCCTCAAGATGGTCGTATTAAATTAAAACTATCTAA
AACCAAAGCCATTGATTTTCGTGTGAACTCACTCCCAACCCTCTTTGGTGAAAAACTGGTACTGCGTATTCTTGATCCAT
CCAGCGCCATGCTCGGGATTGATGCCTTAGGCTATGAAGAACATCAAAAAGCCTTATTTATGGAAGCGCTCGAAAAACCT
CAAGGCATGTTACTTATCACAGGTCCAACGGGTTCAGGTAAAACGGTTTCTTTATATACAGGCTTGAATATTTTAAACAC
CGAAAGCTCGAATATTTCTACAGCCGAAGACCCTGTCGAAATCAACTTGGAAGGGATTAACCAAGTTAACGTCAATCCTA
AAGTGGGTTTAAGCTTCTCCGCAGCGCTCAAATCATTCCTGCGTCAAGATCCAGATATCATCATGGTCGGGGAGATTCGA
GACCTTGAAACTGCTGAAATTGCGATTAAGGCAGCACAGACCGGTCATATGGTGATGTCTACTCTACATACCAATAGTGC
TCCTGAAACATTGACCCGTTTAAGAAACATGGGGGTGCCTTCTTTTAACATCGCAACATCAGTGAATCTCGTGATTGCTC
AACGATTGGCGCGTCGCCTATGTAGTCACTGTAAAGCGCCCGTTGAAATTCCAAAGCAAAGTCTGTTAGAAATGGGTTTT
ACAGAACAAGATTTAAGCAACCCAGACTTACAAATATATCAACCGATTGGCTGTAGTGAATGTCGTGAAGGCTATAAGGG
GCGTGTTGGTGTATATGAAGTGATGAAAGTAACACCTGAAATTTCAAAGATTATTATGGAAGATGGTAATGCGCTTCAAA
TCGCAGCAGCATCTGAGAAATTGGGGTTTAATAATCTAAGACGTTCAGGACTGATTAAGGTCATGAATGGTTTAACTTCT
TTACAAGAAATTAACCGCGTAACCAGCGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

78.984

100

0.791
  pilB Acinetobacter baylyi ADP1

74.386

100

0.744
  pilB Legionella pneumophila strain ERS1305867

51.304

100

0.518
  pilF Neisseria gonorrhoeae MS11

49.466

98.596

0.488
  pilB Vibrio parahaemolyticus RIMD 2210633

48.746

97.895

0.477
  pilB Vibrio campbellii strain DS40M4

47.594

98.421

0.468
  pilB Vibrio cholerae strain A1552

48.282

97.018

0.468
  pilB/pilB1 Synechocystis sp. PCC 6803

35.458

100

0.381

Multiple sequence alignment