Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   D0B54_RS18910 Genome accession   NZ_CP031704
Coordinates   4196222..4197955 (+) Length   577 a.a.
NCBI ID   WP_117293103.1    Uniprot ID   A0A346N4Y7
Organism   Solimonas sp. K1W22B-7     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4191222..4202955
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0B54_RS18890 (D0B54_18890) - 4193095..4193763 (+) 669 WP_205527178.1 class I SAM-dependent methyltransferase -
  D0B54_RS18895 (D0B54_18895) - 4193763..4194164 (+) 402 WP_117293097.1 GtrA family protein -
  D0B54_RS18900 (D0B54_18900) - 4194161..4195129 (+) 969 WP_117293099.1 fatty acid desaturase -
  D0B54_RS18905 (D0B54_18905) - 4195147..4196127 (+) 981 WP_162932562.1 class I SAM-dependent methyltransferase -
  D0B54_RS18910 (D0B54_18910) pilB 4196222..4197955 (+) 1734 WP_117293103.1 type IV-A pilus assembly ATPase PilB Machinery gene
  D0B54_RS18915 (D0B54_18915) pilC 4198064..4199239 (+) 1176 WP_240433655.1 type II secretion system F family protein Machinery gene
  D0B54_RS18920 (D0B54_18920) pilD 4199264..4200124 (+) 861 WP_117293107.1 A24 family peptidase Machinery gene
  D0B54_RS18925 (D0B54_18925) coaE 4200124..4200771 (+) 648 WP_117293109.1 dephospho-CoA kinase -
  D0B54_RS18930 (D0B54_18930) zapD 4200807..4201583 (+) 777 WP_117293111.1 cell division protein ZapD -
  D0B54_RS18935 (D0B54_18935) - 4201580..4201786 (+) 207 WP_162932563.1 DNA gyrase inhibitor YacG -
  D0B54_RS18940 (D0B54_18940) - 4201899..4202840 (-) 942 WP_162932564.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62457.57 Da        Isoelectric Point: 5.0712

>NTDB_id=310637 D0B54_RS18910 WP_117293103.1 4196222..4197955(+) (pilB) [Solimonas sp. K1W22B-7]
MAISPQTPTPTAFLGGLARRLVADGILSEDKGRELQQQAMKSGTPFVKLLVDSKLASPARVAEAASTEFGVPLVDLSTIE
IDSAAFKEVSEKVMRKSHALPIFKRGKKMYVCVSDPTNLSALDEIKFATGNMTEAILVEEDKLAKAIDAAINALNASDMT
VEDEDLADIDISDGSEDKGPEVTRSDADDAPIVKFVNKILIDAINQGASDVHFEPYEKTFRIRYRRDGELKTISQPPISQ
AVRIAARVKVMSRLDLAERRVPQDGRIKLHLSKTKAIDFRVSTCPTLFGEKICCRILDPSSAQLGIDALGYEAEQKEAYM
KSLAEPQGMILVTGPTGSGKTVSLYTGLNILNTDDINISTAEDPAEINLPGINQVNVQPKVGLTFAAALKAFLRQDPDVI
MVGEIRDLETAEIAIKAAQTGHLVLSTLHTNDAPQTIVRLMNMGVPAYNIASTLSLIIAQRLARRLCKICKRPANIPRAE
LIKQGFTEQEVDTPGFTTYEAVGCDACDNGYKGRTGIYQVMPFSDAMGRIIMEGGNAIEIADQSAKEGILDLRQSALRKV
KDGHIDLAQANACTAGD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=310637 D0B54_RS18910 WP_117293103.1 4196222..4197955(+) (pilB) [Solimonas sp. K1W22B-7]
ATGGCCATCAGCCCTCAAACCCCGACCCCGACCGCCTTCCTCGGCGGCCTGGCCCGTCGCCTGGTGGCGGACGGCATCCT
GAGCGAGGACAAGGGCCGCGAGTTGCAGCAGCAGGCCATGAAGTCCGGGACCCCCTTCGTCAAGCTGCTGGTGGACTCCA
AGCTGGCCTCCCCGGCGCGGGTGGCCGAGGCCGCCAGCACCGAGTTCGGCGTACCGCTGGTGGACCTGTCGACCATCGAG
ATCGACTCCGCGGCCTTCAAGGAGGTCAGCGAGAAGGTCATGCGCAAGTCGCATGCCCTGCCGATCTTCAAACGCGGCAA
GAAGATGTACGTCTGCGTTTCCGACCCCACCAACCTCTCGGCCCTGGACGAGATCAAGTTCGCCACCGGCAACATGACCG
AGGCGATCCTGGTTGAGGAAGACAAGCTGGCCAAGGCGATCGACGCCGCCATCAATGCCCTCAATGCCTCGGACATGACG
GTCGAGGACGAAGACCTCGCCGACATCGACATCTCCGACGGCAGCGAGGACAAGGGCCCTGAGGTCACGCGCTCGGACGC
CGACGATGCCCCGATCGTCAAGTTCGTCAACAAGATCCTGATCGACGCCATCAACCAGGGCGCCTCCGACGTCCATTTCG
AGCCCTACGAAAAGACCTTCCGCATCCGCTACCGCCGCGACGGCGAGCTCAAGACCATCTCCCAGCCGCCGATCAGCCAG
GCCGTGCGCATCGCCGCCCGCGTCAAGGTCATGTCGCGCCTGGACCTGGCCGAGCGCCGCGTGCCGCAGGACGGACGCAT
CAAGCTGCACCTGTCCAAGACCAAGGCGATCGACTTCCGCGTCAGCACCTGCCCGACGCTGTTCGGCGAAAAGATCTGCT
GCCGTATCCTCGACCCCAGCAGCGCGCAGCTCGGCATCGACGCGCTGGGCTACGAGGCGGAGCAGAAAGAGGCCTACATG
AAGTCCCTGGCGGAGCCCCAGGGCATGATCCTGGTCACCGGCCCCACCGGCTCCGGCAAGACCGTGTCGCTGTACACCGG
CCTGAACATCCTCAACACCGACGACATCAACATCTCCACGGCGGAGGACCCGGCTGAAATCAACCTGCCGGGCATCAACC
AGGTCAACGTGCAGCCCAAGGTCGGCCTGACCTTCGCCGCCGCGCTCAAGGCCTTCCTGCGCCAGGACCCCGACGTCATC
ATGGTCGGCGAAATCCGCGACCTGGAAACCGCTGAGATCGCGATCAAGGCGGCGCAGACCGGGCATCTGGTGCTGTCCAC
CCTGCACACCAACGACGCGCCGCAGACCATCGTGCGCCTGATGAACATGGGCGTGCCGGCCTACAACATCGCCTCGACGC
TGTCGCTGATCATCGCGCAGCGCCTGGCACGCCGCCTGTGCAAGATCTGCAAGCGCCCCGCCAACATCCCGCGCGCCGAG
CTGATCAAGCAGGGCTTCACCGAACAGGAAGTCGACACCCCGGGCTTCACCACCTATGAAGCGGTGGGCTGCGATGCCTG
CGACAACGGCTACAAGGGCCGTACCGGCATCTACCAGGTAATGCCGTTCAGCGACGCCATGGGACGTATCATCATGGAAG
GCGGCAACGCCATCGAGATCGCCGACCAGTCTGCCAAGGAAGGTATACTTGACCTGCGGCAATCGGCACTCAGGAAAGTC
AAGGACGGACACATCGATCTCGCCCAGGCCAACGCCTGCACGGCGGGAGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-149)

Type II/IV secretion system protein (T2SSE)

(198-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A346N4Y7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.357

97.054

0.537
  pilB Acinetobacter baylyi ADP1

54.306

98.614

0.536
  pilB Legionella pneumophila strain ERS1305867

51.675

98.267

0.508
  pilB Vibrio cholerae strain A1552

49.451

94.627

0.468
  pilF Neisseria gonorrhoeae MS11

47.865

97.4

0.466
  pilB Vibrio campbellii strain DS40M4

47.406

96.88

0.459
  pilB Vibrio parahaemolyticus RIMD 2210633

47.913

95.494

0.458
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.639

100

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

34.578

100

0.369

Multiple sequence alignment