Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   CH621_RS04050 Genome accession   NZ_CP031683
Coordinates   778714..779406 (+) Length   230 a.a.
NCBI ID   WP_014550482.1    Uniprot ID   -
Organism   Haemophilus influenzae strain P662-7189     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 773714..784406
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CH621_RS04030 (CH621_04030) ampD 774988..775548 (-) 561 WP_011271974.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CH621_RS04035 (CH621_04035) pilA 775663..776112 (+) 450 WP_011271973.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  CH621_RS04040 (CH621_04040) pilB 776109..777503 (+) 1395 WP_011271972.1 GspE/PulE family protein Machinery gene
  CH621_RS04045 (CH621_04045) pilC 777500..778717 (+) 1218 WP_014550481.1 type II secretion system F family protein Machinery gene
  CH621_RS04050 (CH621_04050) pilD 778714..779406 (+) 693 WP_014550482.1 A24 family peptidase Machinery gene
  CH621_RS04055 (CH621_04055) rho 779460..780722 (-) 1263 WP_005648966.1 transcription termination factor Rho -
  CH621_RS04060 (CH621_04060) metJ 780970..781287 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  CH621_RS04065 (CH621_04065) cueR 781301..781687 (-) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  CH621_RS04070 (CH621_04070) - 781764..781970 (+) 207 WP_005666692.1 heavy-metal-associated domain-containing protein -
  CH621_RS04075 (CH621_04075) - 782044..782250 (+) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -
  CH621_RS04080 (CH621_04080) - 782225..784393 (+) 2169 WP_042594231.1 heavy metal translocating P-type ATPase -

Sequence

Protein


Download         Length: 230 a.a.        Molecular weight: 26683.67 Da        Isoelectric Point: 8.5626

>NTDB_id=309959 CH621_RS04050 WP_014550482.1 778714..779406(+) (pilD) [Haemophilus influenzae strain P662-7189]
MIYFTMFLLGGILGIALWFYLSGFITRLQQEIYATYIELFPQNFLSFQPSVSSIQQKKCGHILRYFLNVGVEFIFLQIAF
KDSTFAIWLGLTLLILWAISYLDWHYQLISTTLCLWLLTIGLFGADNNFSLLTLSESIKSAASFFIVFYAIYWIAKFYYG
KEAFGRGDYWLAMALGSFIHLETLPHFLLLASVLGICFSLIHKKKKEFIPFAPFMNLSAVIIYFVKYYGY

Nucleotide


Download         Length: 693 bp        

>NTDB_id=309959 CH621_RS04050 WP_014550482.1 778714..779406(+) (pilD) [Haemophilus influenzae strain P662-7189]
ATGATTTACTTCACAATGTTTTTATTAGGCGGTATCTTAGGGATCGCATTGTGGTTCTACCTATCTGGTTTTATTACCCG
TTTACAGCAAGAGATTTATGCGACTTATATTGAATTATTTCCACAAAACTTTCTGTCATTTCAGCCTAGCGTTTCCTCTA
TTCAACAAAAAAAGTGCGGTCATATTTTGAGGTATTTTTTAAATGTTGGGGTAGAATTTATATTTTTACAAATTGCCTTC
AAGGACTCTACTTTCGCTATATGGCTAGGGCTCACACTTCTTATCCTTTGGGCAATCAGTTATCTTGATTGGCACTATCA
ACTTATTTCTACTACACTCTGTTTATGGCTACTTACAATCGGTTTATTTGGAGCAGACAATAATTTTTCATTGCTTACGT
TATCTGAAAGCATAAAAAGTGCGGCAAGTTTTTTTATTGTTTTCTACGCAATCTATTGGATTGCAAAATTCTATTACGGA
AAAGAAGCCTTTGGACGGGGAGATTATTGGCTAGCAATGGCATTAGGAAGTTTTATTCATTTAGAAACCTTACCGCACTT
TTTATTATTAGCCTCAGTTCTTGGAATATGTTTTTCGCTTATTCATAAAAAGAAAAAAGAATTTATACCTTTTGCCCCTT
TTATGAACTTATCAGCTGTCATCATTTATTTCGTCAAATATTACGGATATTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(92-199)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Haemophilus influenzae 86-028NP

89.565

100

0.896
  pilD Haemophilus influenzae Rd KW20

89.13

100

0.891

Multiple sequence alignment