Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DVV14_RS09565 Genome accession   NZ_CP031472
Coordinates   2119617..2121263 (+) Length   548 a.a.
NCBI ID   WP_064488043.1    Uniprot ID   -
Organism   Vibrio coralliilyticus strain RE22     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2114617..2126263
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DVV14_RS09535 (DVV14_09590) - 2114882..2115634 (-) 753 WP_006958128.1 thioredoxin fold domain-containing protein -
  DVV14_RS09540 (DVV14_09595) xerD 2115674..2116570 (-) 897 WP_129542214.1 site-specific tyrosine recombinase XerD -
  DVV14_RS09545 (DVV14_09600) fldB 2116734..2117255 (+) 522 WP_038163561.1 flavodoxin FldB -
  DVV14_RS09550 (DVV14_09605) ampD 2117382..2117927 (-) 546 WP_064488041.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DVV14_RS09555 (DVV14_09610) nadC 2118059..2118946 (+) 888 WP_019277527.1 carboxylating nicotinate-nucleotide diphosphorylase -
  DVV14_RS09560 (DVV14_09615) - 2119174..2119614 (+) 441 WP_064488042.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  DVV14_RS09565 (DVV14_09620) pilB 2119617..2121263 (+) 1647 WP_064488043.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DVV14_RS09570 (DVV14_09625) - 2121272..2122486 (+) 1215 WP_064488044.1 type II secretion system F family protein -
  DVV14_RS09575 (DVV14_09630) pilD 2122536..2123405 (+) 870 WP_064488045.1 A24 family peptidase Machinery gene
  DVV14_RS09580 (DVV14_09635) coaE 2123407..2124015 (+) 609 WP_064488046.1 dephospho-CoA kinase -
  DVV14_RS09585 (DVV14_09640) zapD 2124049..2124789 (+) 741 WP_043009234.1 cell division protein ZapD -
  DVV14_RS09590 (DVV14_09645) yacG 2124841..2125035 (+) 195 WP_019277521.1 DNA gyrase inhibitor YacG -
  DVV14_RS09595 (DVV14_09650) rplS 2125259..2125612 (-) 354 WP_006958143.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 548 a.a.        Molecular weight: 60702.08 Da        Isoelectric Point: 4.8489

>NTDB_id=307050 DVV14_RS09565 WP_064488043.1 2119617..2121263(+) (pilB) [Vibrio coralliilyticus strain RE22]
MSELPFLLKKANKISEEQALQAISATETRQIEAYSALLEISDLTSKQLTAAISELYALPILSLDDFDYRTCCEQLGIGDL
IRSHTALPLEVERQHLTLALADPSISEIEQEFRFATGLAIKLVLAELPTLARAINNLYGAQSASDSLGYKEVSALDLEQL
VEAEEHELIDSEDLNHDSSPISRYIQQILLNAVRKQASDIHFEPHESWFRVRFRCDGLLVEVQRPPQHLCQRLTARLKIL
AKLDIAERRLPQDGRITLNVTQTDSIDIRVSTLPTIWGEKIVLRLLDNRMTSFDLSSLGYNHIQNESYRQALTKPQGLIL
ITGPTGSGKTLSLYSGLNIINQNEINIATAEDPVEIHLTGINQVQIQPQIGFDFAQALRAFLRQDPDVVMVGEIRDRETA
EIATKAAQTGHLVLSTLHTNSAAEAILRLQNMGIEAFNLAASLSLIIAQRLIRCLCPQCKRPTKTQTGHCEASSEGCQDC
NQGYSGRTGIYEVLTVTPEIAQAINDGATANQIEDLAIQQGMQTLPHSGLEKLAQGLTSKEELQRVLF

Nucleotide


Download         Length: 1647 bp        

>NTDB_id=307050 DVV14_RS09565 WP_064488043.1 2119617..2121263(+) (pilB) [Vibrio coralliilyticus strain RE22]
ATGAGTGAACTGCCCTTCTTACTAAAAAAAGCCAATAAAATTAGTGAAGAACAAGCCCTGCAAGCAATATCAGCTACAGA
AACCCGACAAATTGAGGCGTATTCTGCTCTGCTAGAGATTAGTGACCTGACATCCAAACAGCTGACAGCAGCTATCAGTG
AACTCTATGCTCTCCCTATCTTGTCGTTAGATGACTTCGATTATCGAACTTGTTGCGAGCAGCTCGGCATTGGCGACTTA
ATTCGCAGCCACACGGCACTGCCACTAGAAGTAGAGCGACAACATCTTACTCTAGCGCTCGCCGATCCTTCCATTTCCGA
GATAGAACAAGAATTTCGTTTTGCAACTGGCCTAGCAATCAAACTCGTATTGGCCGAGTTACCGACTTTAGCCCGTGCGA
TAAACAACCTATATGGAGCTCAAAGCGCCAGTGACTCTTTAGGCTACAAAGAGGTTAGCGCCCTAGACCTTGAGCAGTTA
GTCGAAGCTGAAGAACATGAATTAATAGACAGTGAGGATCTCAATCACGATAGCTCGCCAATCAGTCGCTATATCCAGCA
GATACTGCTGAATGCCGTACGTAAGCAAGCTTCTGATATCCATTTTGAACCTCATGAATCATGGTTTCGCGTCAGGTTTC
GTTGTGATGGCTTGCTTGTTGAAGTGCAGCGACCTCCTCAGCACTTATGCCAAAGGTTAACCGCCAGACTCAAAATCCTC
GCTAAGCTGGACATCGCCGAGCGCAGACTCCCGCAAGATGGCCGTATCACTCTCAATGTGACTCAAACTGATTCTATCGA
CATACGTGTATCGACTCTGCCCACCATATGGGGTGAAAAAATTGTCCTTAGATTGCTCGACAACAGAATGACCAGCTTTG
ACTTAAGCAGCCTTGGCTACAATCACATTCAAAACGAAAGCTATCGTCAGGCGCTGACCAAGCCTCAGGGACTCATTCTA
ATCACTGGCCCCACGGGGAGCGGTAAGACTCTGTCTTTATACAGTGGACTTAACATCATCAATCAGAATGAGATCAACAT
CGCGACGGCAGAAGATCCCGTAGAGATTCACCTAACTGGAATCAATCAGGTGCAAATTCAACCTCAAATCGGCTTTGATT
TCGCTCAGGCACTCAGAGCCTTTCTACGTCAGGATCCCGATGTGGTTATGGTGGGTGAAATTCGCGATCGAGAAACTGCC
GAGATAGCCACCAAAGCGGCTCAAACAGGCCATTTAGTCCTTTCAACACTCCATACCAACTCAGCAGCAGAAGCTATTCT
TCGCCTCCAGAATATGGGAATAGAAGCTTTCAACCTTGCTGCTTCTCTTAGCCTCATCATTGCCCAGCGTTTAATCAGAT
GCCTATGCCCTCAATGCAAGCGCCCTACAAAAACCCAGACTGGCCACTGCGAAGCTTCTTCTGAAGGTTGTCAGGACTGC
AACCAAGGCTACTCAGGACGAACTGGTATTTATGAAGTACTCACAGTGACACCTGAAATTGCGCAAGCTATTAACGACGG
CGCCACAGCTAACCAGATTGAGGACTTAGCGATACAACAAGGCATGCAAACATTACCGCACTCGGGCTTAGAAAAATTGG
CCCAAGGTCTCACCAGTAAGGAAGAACTGCAGCGAGTGCTGTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(187-453)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

55.893

100

0.571
  pilB Vibrio parahaemolyticus RIMD 2210633

55.914

100

0.569
  pilB Vibrio campbellii strain DS40M4

55.635

100

0.568
  pilB Legionella pneumophila strain ERS1305867

47.025

95.073

0.447
  pilB Acinetobacter baylyi ADP1

45.817

95.985

0.44
  pilB Acinetobacter baumannii D1279779

44.177

98.723

0.436
  pilF Neisseria gonorrhoeae MS11

41.843

95.073

0.398
  pilB Haemophilus influenzae Rd KW20

44.017

85.401

0.376
  pilB Haemophilus influenzae 86-028NP

43.376

85.401

0.37
  pilB Glaesserella parasuis strain SC1401

40.909

88.321

0.361

Multiple sequence alignment