Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   DV311_RS09275 Genome accession   NZ_CP031253
Coordinates   1692574..1694250 (-) Length   558 a.a.
NCBI ID   WP_114935779.1    Uniprot ID   -
Organism   Neisseria lactamica strain M17106     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1687574..1699250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV311_RS09255 pilG 1689487..1690719 (+) 1233 WP_003710974.1 type 4 pilus assembly protein PilG Machinery gene
  DV311_RS09260 pilD 1690793..1691653 (+) 861 WP_114935776.1 prepilin peptidase Machinery gene
  DV311_RS09265 coaE 1691655..1692287 (+) 633 WP_114935777.1 dephospho-CoA kinase -
  DV311_RS09270 - 1692280..1692489 (+) 210 WP_114935778.1 DNA gyrase inhibitor YacG -
  DV311_RS09275 pilF 1692574..1694250 (-) 1677 WP_114935779.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DV311_RS09290 - 1694882..1695328 (-) 447 WP_003710982.1 DUF441 domain-containing protein -
  DV311_RS09295 - 1695333..1696307 (-) 975 WP_003710984.1 polyprenyl synthetase family protein -
  DV311_RS09305 rplU 1696531..1696839 (+) 309 WP_002216394.1 50S ribosomal protein L21 -
  DV311_RS09310 rpmA 1696865..1697137 (+) 273 WP_002212328.1 50S ribosomal protein L27 -
  DV311_RS09315 ubiM 1697522..1698706 (+) 1185 WP_003714013.1 5-demethoxyubiquinol-8 5-hydroxylase UbiM -
  DV311_RS09320 rpmG 1698895..1699050 (-) 156 WP_003689824.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62033.12 Da        Isoelectric Point: 5.4107

>NTDB_id=305069 DV311_RS09275 WP_114935779.1 1692574..1694250(-) (pilF) [Neisseria lactamica strain M17106]
MSVGLLRTLVQNQVVTVEQAEHYYKESQAGKEVLPMLFSDGVISPKSLAALIARVFSYSVLDLRHYPRHRVLMGVLTEEQ
MVEFHCVPVFRRDNKVFFAVSDPTQMPQIQKTVSAAGIAVELVIVEDDQLAGLLDWVGSRSTSLLQELGEEQEEEESHTL
YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPIAVRGQLASRIKVMSRLDISEKR
IPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSG
KTVSLYTCLNILNTESVNIATAEDPAEINLPGINQVNVNEKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQ
TGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEIGFTDEDLAKDWKLY
HAVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=305069 DV311_RS09275 WP_114935779.1 1692574..1694250(-) (pilF) [Neisseria lactamica strain M17106]
ATGAGCGTAGGTTTGCTGAGAACTTTGGTTCAAAACCAAGTGGTTACTGTCGAACAGGCCGAACACTATTATAAGGAGTC
GCAGGCGGGTAAAGAAGTGTTGCCGATGCTGTTTTCAGACGGTGTCATTTCGCCTAAATCCCTTGCGGCACTGATTGCCA
GGGTGTTCAGTTATTCGGTTCTTGATTTGCGCCATTATCCGCGCCACAGGGTGCTGATGGGGGTGTTGACGGAAGAGCAG
ATGGTGGAGTTCCACTGTGTGCCGGTTTTCCGTCGGGACAATAAAGTATTTTTTGCGGTTTCCGATCCGACCCAGATGCC
GCAAATTCAGAAAACCGTGTCTGCCGCAGGGATTGCGGTTGAGTTGGTCATTGTCGAGGATGACCAGTTGGCGGGTTTGC
TCGATTGGGTGGGGTCGAGATCGACATCGCTGCTTCAGGAGCTTGGGGAGGAGCAGGAGGAAGAGGAAAGCCACACCCTG
TATATCGACAATGAGGAGGCGGAGGACGGCCCTGTTCCGAGGTTTATCCATAAAACTTTGTCGGATGCCTTGCGCAGCGG
GGCATCGGACATTCATTTTGAGTTTTACGAACACAATGCCCGTATCCGTTTCCGCGTGGACGGGCAGCTTCGCGAGGTGG
TTCAGCCGCCTATTGCGGTAAGGGGGCAGCTTGCTTCCCGGATTAAGGTGATGTCGCGTTTGGATATTTCCGAAAAACGC
ATACCGCAGGACGGCAGGATGCAGCTGACCTTTCAAAAGGGCGGCAAGCCTGTCGATTTCCGCGTCAGCACTTTGCCGAC
GCTGTTTGGCGAAAAGGTTGTGATGCGGATTTTGAACTCCGATGCCGCGTCTTTGAATATCGACCAGCTCGGTTTTGAGC
CGTTTCAGAAAAAATTGCTGTTGGAAGCGATTCATCGTCCTTACGGTATGGTGCTGGTAACCGGCCCGACGGGTTCGGGT
AAGACGGTGTCGCTCTATACCTGTTTGAATATTTTGAATACGGAGTCGGTAAATATTGCAACGGCGGAAGACCCTGCCGA
GATTAACCTGCCGGGCATCAATCAGGTCAACGTCAATGAGAAGCAGGGTCTGACTTTTGCCGCTGCTTTAAAGTCTTTCC
TGCGTCAGGATCCGGACATCATTATGGTCGGTGAGATTCGTGATTTGGAAACTGCCGATATTGCGATTAAGGCGGCACAA
ACAGGGCATATGGTGTTTTCCACCCTGCACACCAATAATGCGCCGGCGACGTTGTCGCGTATGCTGAATATGGGTGTCGC
GCCGTTTAATATTGCCAGTTCGGTCAGCCTGATTATGGCGCAGCGTCTTTTACGCAGGTTGTGTTCGAGCTGCAAACAGG
AAGTGGAACGCCCGTCTGCTTCTGCTCTGAAGGAAATCGGCTTCACCGATGAGGATCTTGCAAAAGATTGGAAACTTTAC
CATGCCGTCGGTTGCGACCGTTGCCGGGGTCAGGGTTACAAGGGGCGTGCGGGCGTGTATGAGGTTATGCCCATCAGCGA
AGAAATGCAGCGTGTGATTATGAACAACGGTACGGAAGTGGATATTTTGGACGTTGCCTATAAGGAGGGTATGGTGGATT
TGCGCCGGGCCGGTATTTTGAAAGTTATGCAGGGCATTACTTCATTGGAAGAGGTAACGGCAAATACCAACGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(178-446)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

98.029

100

0.98
  pilB Acinetobacter baumannii D1279779

50.177

100

0.507
  pilB Acinetobacter baylyi ADP1

50.178

100

0.505
  pilB Legionella pneumophila strain ERS1305867

47.527

100

0.482
  pilB Vibrio cholerae strain A1552

45.374

100

0.457
  pilB Vibrio parahaemolyticus RIMD 2210633

45.213

100

0.457
  pilB Vibrio campbellii strain DS40M4

43.972

100

0.444
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.179

96.057

0.376
  pilF Thermus thermophilus HB27

36.949

97.491

0.36

Multiple sequence alignment