Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   DV446_RS06880 Genome accession   NZ_CP031252
Coordinates   1351348..1353027 (-) Length   559 a.a.
NCBI ID   WP_074894936.1    Uniprot ID   -
Organism   Neisseria elongata strain M15911     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1346348..1358027
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV446_RS06845 - 1346921..1347139 (+) 219 WP_003769492.1 SlyX family protein -
  DV446_RS06850 rpmE 1347274..1347492 (+) 219 WP_049248635.1 50S ribosomal protein L31 -
  DV446_RS06855 - 1347648..1347842 (-) 195 WP_049253000.1 DNA gyrase inhibitor YacG -
  DV446_RS06860 zapD 1347839..1348600 (-) 762 WP_074894942.1 cell division protein ZapD -
  DV446_RS06865 coaE 1348615..1349217 (-) 603 WP_074894940.1 dephospho-CoA kinase -
  DV446_RS06870 pilD 1349217..1350089 (-) 873 WP_074894938.1 prepilin peptidase Machinery gene
  DV446_RS06875 pilG 1350100..1351323 (-) 1224 WP_049248630.1 type II secretion system F family protein Machinery gene
  DV446_RS06880 pilF 1351348..1353027 (-) 1680 WP_074894936.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DV446_RS06885 - 1353159..1354748 (-) 1590 WP_074894934.1 inorganic phosphate transporter -
  DV446_RS06890 - 1355182..1356483 (+) 1302 WP_074894932.1 ATP-binding protein -
  DV446_RS06895 - 1356698..1357687 (+) 990 WP_074894931.1 NAD(P)H-dependent glycerol-3-phosphate dehydrogenase -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62131.44 Da        Isoelectric Point: 5.7232

>NTDB_id=305024 DV446_RS06880 WP_074894936.1 1351348..1353027(-) (pilF) [Neisseria elongata strain M15911]
MSMGLLRVLVQNKTLSSEQAGRYQQALKDNKDIVAMLFADGIVNPKALAELLAGMFNYPVLDLSYYPRSNIVQDVLNEEQ
MQANACVPIFKRGNKIFLAVSDPTRIQEFQKVVLSSGFSIDLVIVRHDQLMTLVEWLGQRSTTILREMSQDQLDVGASQS
LYIDNEEAEDGPIARFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAIRGQLASRIKVMARLDISEK
RIPQDGRIQIAFSKHGRPTDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEDFQKELLLEAIHRPYGMVLVTGPTGS
GKTVSLYTCLNILNTEGVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAS
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSNCKEPMERPPEHALRKAGFSDEDLAKDWTM
YRAVGCDTCRGKGYKGRAGVYEVMPITDEMQRVIMEGGTEVDIQKMAYQEGMVDLRRAGLLKVMSGITSLEEVLAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=305024 DV446_RS06880 WP_074894936.1 1351348..1353027(-) (pilF) [Neisseria elongata strain M15911]
ATGAGTATGGGATTATTAAGGGTTTTGGTTCAGAATAAAACCCTCAGCAGTGAACAAGCTGGACGCTATCAACAAGCATT
GAAAGATAATAAAGATATTGTTGCTATGTTGTTTGCTGACGGGATAGTTAATCCCAAGGCGTTGGCAGAATTGTTAGCGG
GGATGTTCAATTACCCTGTTCTTGATTTGAGTTACTATCCACGTAGTAATATCGTGCAAGATGTTTTAAATGAAGAACAA
ATGCAGGCTAATGCGTGTGTTCCTATTTTTAAGCGTGGTAATAAGATATTCCTTGCGGTATCAGATCCAACTAGAATTCA
AGAGTTTCAAAAAGTCGTTCTTTCTTCCGGATTTTCAATAGATTTGGTGATTGTTCGTCATGATCAGTTGATGACGTTAG
TTGAGTGGCTGGGGCAGAGATCTACTACTATCTTGCGTGAGATGTCCCAAGATCAGTTGGATGTTGGAGCATCGCAATCA
CTATACATTGATAATGAAGAGGCCGAGGATGGGCCTATTGCGCGATTTATTCATAAAACTTTATCTGATGCATTAAATGC
AGGTGCTTCTGATATCCATTTTGAGTTTTATGAGCAGATGGCACGTGTGCGGTTCCGTGTGGATGGGCAATTGCGAGAAG
TTGTGCAACCTCCTGTTGCAATACGTGGGCAATTGGCGTCACGTATTAAGGTGATGGCAAGATTAGATATTTCTGAAAAA
CGAATTCCTCAAGATGGACGCATCCAAATTGCTTTTTCTAAACATGGTAGACCCACAGATTTTCGTGTGAGTACATTGCC
AACGTTGTTTGGTGAAAAAGTTGTGATGCGTATTCTTAATTCGGATGCAGCATCATTGAATATTGATCAGCTTGGATTTG
AAGATTTCCAAAAAGAATTATTATTGGAGGCAATTCATCGACCGTATGGGATGGTCTTAGTAACTGGGCCTACAGGTTCT
GGTAAAACTGTTTCGCTGTATACTTGTTTGAATATTCTAAATACTGAAGGGGTAAATATTTCTACGGCAGAAGATCCTGC
AGAGATTAATTTACCTGGAATTAATCAGGTAAATGTAAATGACAAACAAGGGTTAACTTTTGCTGCTGCACTAAAATCGT
TTCTGCGCCAAGATCCGGATATTATTATGGTTGGTGAGATTCGTGATTTGGAAACTGCAGATATTGCTATTAAAGCATCG
CAGACAGGGCATATGGTGTTTTCTACATTGCATACCAATAATGCTCCTGCAACATTGTCTCGTATGTTGAATATGGGTGT
TGCACCATTTAATATAGCCAGTTCCGTTAGTCTCATTATGGCGCAAAGGTTATTGCGTAGATTGTGTTCAAATTGTAAGG
AACCAATGGAAAGACCTCCTGAGCATGCCTTGAGAAAGGCAGGTTTTTCAGATGAAGATTTAGCTAAAGATTGGACAATG
TATCGAGCGGTAGGTTGTGATACATGCAGAGGTAAAGGTTATAAGGGGCGTGCTGGTGTATATGAAGTAATGCCAATTAC
AGATGAAATGCAAAGAGTGATTATGGAAGGTGGAACAGAAGTAGATATTCAAAAAATGGCATATCAAGAGGGAATGGTAG
ATCTACGTCGAGCAGGTTTATTGAAGGTAATGAGCGGTATTACCTCTCTAGAAGAAGTATTAGCTCATACCAATGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(179-447)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

75.671

100

0.757
  pilB Acinetobacter baumannii D1279779

51.957

100

0.522
  pilB Acinetobacter baylyi ADP1

50.984

100

0.51
  pilB Vibrio cholerae strain A1552

46.864

100

0.481
  pilB Legionella pneumophila strain ERS1305867

47.772

100

0.479
  pilB Vibrio parahaemolyticus RIMD 2210633

47.249

94.275

0.445
  pilB Vibrio campbellii strain DS40M4

45.541

94.275

0.429
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.076

93.739

0.376
  pilF Thermus thermophilus HB27

38.258

94.454

0.361

Multiple sequence alignment