Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   soil367_RS11585 Genome accession   NZ_CP031093
Coordinates   2600476..2602191 (+) Length   571 a.a.
NCBI ID   WP_136549248.1    Uniprot ID   A0A4P7XHL9
Organism   Hydrocarboniclastica marina strain soil36-7     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2595476..2607191
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  soil367_RS11550 (soil367_11645) - 2596622..2597623 (+) 1002 WP_136549244.1 integrase domain-containing protein -
  soil367_RS11555 (soil367_11650) - 2597620..2597970 (+) 351 WP_136549245.1 hypothetical protein -
  soil367_RS11560 (soil367_11655) - 2598109..2598408 (+) 300 WP_136549246.1 MbcA/ParS/Xre antitoxin family protein -
  soil367_RS11570 (soil367_11665) - 2598701..2598955 (-) 255 WP_136549247.1 hypothetical protein -
  soil367_RS11580 (soil367_11675) - 2599555..2600055 (-) 501 WP_281283906.1 pilin -
  soil367_RS11585 (soil367_11680) pilB 2600476..2602191 (+) 1716 WP_136549248.1 type IV-A pilus assembly ATPase PilB Machinery gene
  soil367_RS11590 (soil367_11685) pilC 2602557..2603777 (+) 1221 WP_136549249.1 type II secretion system F family protein Machinery gene
  soil367_RS11595 (soil367_11690) pilD 2603889..2604767 (+) 879 WP_136549250.1 A24 family peptidase Machinery gene
  soil367_RS11600 (soil367_11695) coaE 2604958..2605557 (+) 600 WP_136549251.1 dephospho-CoA kinase -
  soil367_RS11605 (soil367_11700) yacG 2605608..2605817 (+) 210 WP_136549252.1 DNA gyrase inhibitor YacG -
  soil367_RS11610 (soil367_11705) - 2605978..2606421 (+) 444 WP_136549253.1 HIT family protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62702.70 Da        Isoelectric Point: 4.9736

>NTDB_id=303305 soil367_RS11585 WP_136549248.1 2600476..2602191(+) (pilB) [Hydrocarboniclastica marina strain soil36-7]
MAQLTSNVVLTGLARRFVDDGLLEESVARDAFVQANQNKIPLIAYLVQKDLANSRALAYSAGMEFGVPVLDLAAYLPESM
PEGLVDDKLIRKHNALPLFKRGNRLFIAVSDPTNVQGLDEIKFNTGLSTDAVLVDERGLGEAISRYLDSQDTSMEGMDDA
DLEDVETSGGDEEDESAAIKPSDVDDAPIVKYVNKMLLDAIRQGSSDVHFEPYEKIYRVRYRTDGILREASKPSVKLGSK
IAARVKIMAQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAKLGIDALGYEEDQKELYLNAL
KQPQGMILVTGPTGSGKTVSLYTGINILNTHEINISTAEDPAEINLEGVNQVNVNNRVGLTFAEALRSFLRQDPDVIMVG
EIRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMINMGVPAFNIATSVSLIIAQRLGRRLCSCKEKADIPRDVLLKE
GFTEAQLDAGVTIYRPKGCEKCVGGYKGRVGIYEVVKITPQMSSMIMEEASSIEIAQQAKSEGFRNLRESALRKVMEGVT
SLEEANRVTKD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=303305 soil367_RS11585 WP_136549248.1 2600476..2602191(+) (pilB) [Hydrocarboniclastica marina strain soil36-7]
ATGGCTCAACTAACATCCAACGTCGTTCTCACCGGTCTGGCCCGTCGCTTTGTTGACGATGGTCTGCTGGAGGAGTCTGT
TGCTAGGGATGCCTTTGTTCAGGCGAACCAGAACAAGATTCCGCTCATTGCCTACCTGGTGCAAAAGGATCTGGCAAACA
GCCGTGCGTTGGCTTACTCAGCGGGCATGGAGTTTGGAGTGCCGGTGCTGGACCTGGCGGCGTATTTGCCAGAGAGCATG
CCCGAAGGCTTGGTCGACGATAAGCTGATACGCAAACATAACGCATTGCCTCTCTTTAAGCGGGGCAATCGCCTGTTTAT
TGCTGTGTCCGATCCTACCAACGTGCAGGGCCTGGACGAGATAAAGTTCAACACCGGCCTGAGTACGGATGCGGTGCTCG
TTGACGAACGCGGCCTCGGAGAGGCCATTTCCCGGTACCTCGACTCTCAAGATACTTCCATGGAAGGCATGGACGATGCC
GATCTGGAGGATGTGGAAACGTCCGGTGGGGATGAGGAAGACGAGTCGGCCGCAATAAAGCCTAGCGATGTTGACGATGC
GCCGATCGTTAAATACGTCAACAAGATGTTGCTCGACGCGATTCGCCAGGGGTCGTCGGATGTTCACTTTGAGCCTTACG
AGAAAATCTACCGAGTACGCTACCGGACTGATGGCATTCTGCGGGAGGCTTCCAAGCCGTCCGTTAAACTGGGCAGCAAG
ATTGCGGCCCGAGTAAAGATCATGGCGCAATTGGACATTTCCGAACGTCGGGTGCCCCAGGACGGTCGGATCAAGATGAA
GCTGTCCAAGACCAAGGCCATCGATTTTCGGGTGAACACCCTGCCGACGCTATGGGGTGAGAAAATCGTGCTGCGGATTC
TGGACCCCAGCCAAGCCAAATTGGGCATTGACGCGCTAGGGTACGAGGAAGATCAGAAAGAGCTTTATCTCAACGCTCTT
AAACAGCCCCAGGGTATGATTCTTGTTACGGGACCAACGGGGTCAGGTAAGACCGTTTCCCTTTATACCGGTATAAACAT
TCTCAATACGCACGAAATCAATATATCCACGGCGGAAGACCCGGCGGAGATCAACCTGGAGGGAGTAAACCAGGTCAATG
TGAATAACCGGGTGGGCCTGACCTTTGCTGAAGCACTGCGGTCGTTCCTGCGGCAGGATCCGGATGTGATCATGGTCGGG
GAGATCCGGGACCTGGAGACGGCTAACATCGCCATCAAGGCCGCGCAAACCGGCCACTTGGTGCTTTCTACCCTGCACAC
GAATAGCGCCGCGGAAACGCTGACCCGCATGATCAATATGGGCGTTCCTGCGTTTAACATTGCCACGTCTGTGAGCCTGA
TCATCGCCCAGCGCCTGGGCCGCCGGCTCTGTAGTTGTAAGGAGAAGGCGGATATTCCGCGTGACGTCCTGCTCAAGGAA
GGGTTCACCGAGGCACAGCTGGACGCAGGCGTGACGATATACAGGCCCAAAGGCTGTGAGAAGTGTGTCGGAGGCTACAA
AGGCCGTGTAGGGATCTATGAGGTGGTAAAGATCACGCCGCAGATGTCTTCCATGATTATGGAAGAAGCCAGTTCAATTG
AAATTGCCCAGCAGGCGAAATCTGAAGGCTTCCGTAACCTCCGGGAGTCTGCCTTAAGGAAAGTTATGGAGGGGGTTACG
AGCCTTGAGGAGGCCAACCGGGTTACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)

Type II/IV secretion system protein (T2SSE)

(195-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4P7XHL9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.014

100

0.583
  pilB Acinetobacter baylyi ADP1

56.021

100

0.562
  pilB Legionella pneumophila strain ERS1305867

53.993

100

0.545
  pilB Vibrio parahaemolyticus RIMD 2210633

50.995

96.848

0.494
  pilB Vibrio cholerae strain A1552

55.098

89.317

0.492
  pilB Vibrio campbellii strain DS40M4

49.911

98.599

0.492
  pilF Neisseria gonorrhoeae MS11

48.155

94.921

0.457
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.279

92.294

0.363
  pilB/pilB1 Synechocystis sp. PCC 6803

39.884

90.893

0.363

Multiple sequence alignment