Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   DN409_RS06135 Genome accession   NZ_CP031071
Coordinates   1187237..1189063 (+) Length   608 a.a.
NCBI ID   WP_002134970.1    Uniprot ID   A0A0B5S4T9
Organism   Bacillus mycoides strain BPN401     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1182237..1194063
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DN409_RS06115 (DN409_06110) - 1182448..1183122 (-) 675 WP_002030376.1 TerC family protein -
  DN409_RS06120 (DN409_06115) mecA 1183559..1184242 (+) 684 WP_002030378.1 adaptor protein MecA Regulator
  DN409_RS06125 (DN409_06120) - 1184315..1185859 (+) 1545 WP_061689041.1 cardiolipin synthase -
  DN409_RS06130 (DN409_06125) - 1185951..1187186 (+) 1236 WP_061674936.1 competence protein CoiA -
  DN409_RS06135 (DN409_06130) pepF 1187237..1189063 (+) 1827 WP_002134970.1 oligoendopeptidase F Regulator
  DN409_RS30795 - 1189197..1189370 (-) 174 WP_002086970.1 hypothetical protein -
  DN409_RS06140 (DN409_06135) - 1189601..1190494 (-) 894 WP_002140839.1 ClpXP adapter SpxH family protein -
  DN409_RS06145 (DN409_06140) - 1190494..1190892 (-) 399 WP_000043388.1 hypothetical protein -
  DN409_RS06150 (DN409_06145) - 1191078..1191656 (-) 579 WP_002064261.1 CYTH domain-containing protein -
  DN409_RS06155 (DN409_06150) - 1191801..1192172 (+) 372 WP_002030390.1 hypothetical protein -
  DN409_RS06160 (DN409_06155) - 1192203..1192841 (+) 639 WP_002125951.1 GTP pyrophosphokinase family protein -
  DN409_RS06165 (DN409_06160) - 1192860..1193657 (+) 798 WP_002011365.1 NAD kinase -

Sequence

Protein


Download         Length: 608 a.a.        Molecular weight: 70331.69 Da        Isoelectric Point: 4.6480

>NTDB_id=303206 DN409_RS06135 WP_002134970.1 1187237..1189063(+) (pepF) [Bacillus mycoides strain BPN401]
MSEQNKAKTLPDRNEIEEASTWRLEDIFQTDAEWEKEFQAIKELLPKLTEFKGKLGDSADNLLEALQYEDEISMRLGKLY
TYAHMRYDQDTTNSVYQALNDRATNLYSQVSSSTAYIVPEILSISEDTLQTFLQENRELSVYEHALEEITRQRPHVLSEA
EEALLAEASEVMSASSNTFGMLNNADLKFPSIKGEDGEEVEITHGRYIQFLESDDRRVREDAFKAVFETYGKFKNTFAST
LSGAVKRNNFNARIRKYDSARQAALSNNNIPEAVYDQLVETVNDNLHLLHRYIDIRKRALGLDELHMYDLYTPLVQEVKM
NVKYEEAQDLLLKSLNVLGDEYVEILKEAYENRWVDVYENKGKRSGAYSSGAYGTNPYILMNWHDNVNNLYTLAHEFGHS
VHSYYTRKTQPHVYGDYSIFVAEVASTCNEALLNDYLLKTTEDKKERLYLLNHYLEGFRGTVFRQTMFAEFEHIIHKKVQ
EGHAVTPDMLTEIYYDLNKKYFGDALVIDKEIGLEWSRIPHFYYNYYVYQYATGFSAATALSKQILEEGQPAVERYINEF
LKAGSSDYPIEVLKKAGVDMASPEPVKEALQVFEEKLNELEALLFEEK

Nucleotide


Download         Length: 1827 bp        

>NTDB_id=303206 DN409_RS06135 WP_002134970.1 1187237..1189063(+) (pepF) [Bacillus mycoides strain BPN401]
ATGTCTGAACAAAATAAAGCAAAAACATTACCAGATCGCAATGAGATTGAAGAAGCAAGCACATGGCGATTAGAAGATAT
TTTCCAAACAGATGCAGAATGGGAAAAAGAATTCCAAGCTATTAAAGAGCTATTGCCAAAGTTAACTGAATTTAAAGGGA
AGCTTGGCGACTCTGCAGACAATTTGCTTGAGGCATTGCAATATGAAGATGAAATTTCAATGCGATTAGGTAAGCTATAT
ACATATGCACATATGCGTTACGATCAAGATACAACAAACTCTGTGTATCAAGCACTGAATGATCGCGCAACAAATTTATA
CTCACAAGTATCTAGTAGCACAGCGTATATCGTACCTGAAATTTTATCTATTTCAGAAGATACATTGCAAACATTTTTAC
AAGAAAATCGAGAACTAAGTGTATATGAGCATGCATTAGAAGAAATTACACGTCAACGCCCGCACGTATTATCTGAGGCA
GAGGAAGCGTTATTAGCAGAAGCATCTGAAGTGATGAGTGCATCAAGTAATACATTCGGTATGTTGAATAATGCGGATTT
GAAATTCCCATCTATTAAAGGTGAGGATGGAGAAGAGGTAGAAATAACACATGGCCGTTACATTCAGTTTTTAGAAAGTG
ATGATCGTCGTGTTCGCGAAGATGCATTCAAAGCTGTATTTGAAACGTATGGGAAATTTAAGAACACATTTGCAAGTACG
TTAAGCGGGGCAGTGAAACGTAATAATTTCAATGCACGTATTCGTAAATATGACTCTGCTCGCCAAGCAGCATTAAGCAA
TAATAATATTCCTGAAGCAGTATACGATCAACTCGTTGAAACGGTAAATGACAACTTACATTTATTACACCGTTACATTG
ATATTCGTAAGCGTGCACTAGGACTTGATGAGCTTCATATGTACGATTTATATACACCACTTGTACAAGAAGTGAAAATG
AATGTGAAGTATGAAGAAGCGCAAGACCTGTTATTAAAATCTTTAAACGTACTTGGTGATGAATATGTTGAAATTTTAAA
AGAAGCATATGAAAATCGCTGGGTAGATGTGTACGAGAATAAAGGCAAACGAAGCGGAGCATATTCATCTGGTGCATACG
GAACAAATCCGTATATTTTAATGAACTGGCATGATAATGTAAATAATTTATATACACTTGCTCATGAGTTTGGTCATTCG
GTGCATAGTTATTACACAAGAAAGACACAGCCACACGTATACGGTGATTATTCAATCTTCGTTGCAGAGGTAGCATCAAC
TTGTAATGAAGCGCTTCTAAATGATTATTTATTAAAAACGACAGAAGATAAAAAAGAGCGTCTATATTTATTAAATCATT
ATTTAGAAGGATTCCGTGGTACTGTATTCCGTCAAACAATGTTTGCAGAGTTCGAGCATATCATTCATAAAAAAGTACAA
GAAGGACATGCGGTTACGCCAGATATGTTAACGGAGATCTACTACGATTTAAATAAGAAATATTTCGGGGATGCTTTAGT
AATTGATAAAGAAATCGGTTTAGAATGGTCTCGTATTCCGCACTTCTATTACAACTATTACGTATATCAATACGCAACAG
GATTTAGTGCAGCGACTGCGTTATCTAAACAAATTTTAGAAGAAGGACAACCTGCAGTAGAACGTTACATTAACGAATTC
TTAAAAGCGGGAAGCTCCGATTATCCAATTGAAGTGTTGAAAAAAGCAGGAGTTGATATGGCATCTCCTGAACCAGTGAA
AGAGGCACTTCAAGTATTTGAAGAGAAATTAAATGAATTAGAAGCGTTATTATTTGAAGAAAAGTAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(120-189)

Peptidase family M3 (Peptidase_M3)

(211-591)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B5S4T9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

49.747

97.533

0.485

Multiple sequence alignment