Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   DN399_RS05975 Genome accession   NZ_CP031062
Coordinates   1144694..1146520 (+) Length   608 a.a.
NCBI ID   WP_000003348.1    Uniprot ID   -
Organism   Bacillus sp. AR4-2     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1139694..1151520
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DN399_RS05955 (DN399_05940) - 1139997..1140671 (-) 675 WP_000362607.1 TerC family protein -
  DN399_RS05960 (DN399_05945) mecA 1141017..1141700 (+) 684 WP_000350713.1 adaptor protein MecA Regulator
  DN399_RS05965 (DN399_05950) - 1141773..1143317 (+) 1545 WP_000799194.1 cardiolipin synthase -
  DN399_RS05970 (DN399_05955) - 1143398..1144642 (+) 1245 WP_000628315.1 competence protein CoiA family protein -
  DN399_RS05975 (DN399_05960) pepF 1144694..1146520 (+) 1827 WP_000003348.1 oligoendopeptidase F Regulator
  DN399_RS27995 - 1146650..1146823 (-) 174 WP_000750355.1 hypothetical protein -
  DN399_RS05980 (DN399_05965) - 1147053..1147946 (-) 894 WP_000360645.1 ClpXP adapter SpxH family protein -
  DN399_RS05985 (DN399_05970) - 1147946..1148344 (-) 399 WP_000043386.1 hypothetical protein -
  DN399_RS05990 (DN399_05975) - 1148513..1149091 (-) 579 WP_000191121.1 CYTH domain-containing protein -
  DN399_RS05995 (DN399_05980) - 1149236..1149607 (+) 372 WP_001180003.1 hypothetical protein -
  DN399_RS06000 (DN399_05985) - 1149638..1150276 (+) 639 WP_001081482.1 GTP pyrophosphokinase family protein -
  DN399_RS06005 (DN399_05990) - 1150295..1151092 (+) 798 WP_000673190.1 NAD kinase -

Sequence

Protein


Download         Length: 608 a.a.        Molecular weight: 70199.57 Da        Isoelectric Point: 4.6443

>NTDB_id=303056 DN399_RS05975 WP_000003348.1 1144694..1146520(+) (pepF) [Bacillus sp. AR4-2]
MSEQNKAKALPDRNEIEEASTWRLEDIFQTDAEWEKEFQAIKELLPKLTEFKGKLGDSADNLLEALQYEDEISMRLGKLY
TYAHMRYDQDTTNSVYQALNDRATNLYSQVSSSTAYIVPEILSISEDTLQTFLKENRDLSVYEHALEEITRQRPHVLSEA
EEALLAEASEVMSSSSNTFGMLNNADLKFPSIKGEDGEEIEITHGRYIQFLESDDPRVRKDAFKAVYETYGKFKNTFAST
LSGAVKRNNFNARVRKYDSARQAALSNNNIPEAVYDQLVETVNDNLHLLHRYIDIRKRALGLDELHMYDLYTPLVPEVKM
NVKYEEAQDLLLKSLNVLGDEYVDILKEAYENRWVDVYENKGKRSGAYSSGAYGTNPYILMNWHDNVNNLFTLAHEFGHS
VHSYYTRKTQPHVYGDYSIFVAEVASTCNEALLNDYLLKTTEDKKERLYLLNHYLEGFRGTVFRQTMFAEFEHIIHKKVQ
EGHAVTPDMLTEIYYDLNKKYFGDALVIDEEIGLEWSRIPHFYYNYYVYQYATGFSAATALSKQILEEGQPAVERYINEF
LKAGSSDYPIEVLKKAGVDMASPEPVKEALQVFEEKLNELEALLFEEK

Nucleotide


Download         Length: 1827 bp        

>NTDB_id=303056 DN399_RS05975 WP_000003348.1 1144694..1146520(+) (pepF) [Bacillus sp. AR4-2]
ATGTCTGAACAAAACAAAGCGAAAGCATTACCAGATCGCAACGAGATTGAAGAAGCAAGTACGTGGCGATTAGAAGATAT
TTTCCAAACGGATGCAGAATGGGAAAAAGAATTCCAAGCTATTAAAGAGCTATTACCGAAGTTAACTGAATTTAAAGGGA
AACTTGGTGACTCTGCGGACAATTTACTTGAGGCATTGCAATATGAAGATGAAATATCAATGCGATTAGGTAAGTTATAT
ACATATGCTCATATGCGTTACGATCAAGATACAACAAACTCTGTATATCAAGCATTAAATGATCGTGCAACAAATTTATA
TTCACAAGTATCTAGTAGCACAGCGTATATTGTGCCTGAAATTTTATCGATTTCAGAAGATACATTGCAAACATTCTTGA
AGGAAAATAGAGATTTAAGTGTATATGAACATGCATTAGAAGAAATTACACGTCAACGCCCGCACGTATTATCAGAAGCT
GAGGAAGCTTTATTAGCGGAAGCATCTGAAGTAATGAGTTCATCAAGCAATACATTTGGTATGTTGAATAACGCGGATTT
AAAATTCCCATCTATTAAAGGGGAAGACGGAGAAGAAATAGAAATTACACATGGCCGTTACATTCAGTTCTTAGAAAGTG
ATGATCCTCGTGTTCGCAAAGATGCATTTAAAGCTGTATATGAAACGTACGGAAAATTTAAAAACACATTCGCAAGTACG
TTAAGCGGAGCAGTAAAACGTAATAATTTCAATGCGCGTGTTCGTAAATATGATTCTGCTCGCCAAGCTGCACTGAGCAA
TAATAATATTCCGGAAGCAGTATACGATCAACTCGTTGAAACAGTAAATGACAACTTACATTTATTACACCGTTACATCG
ATATTCGTAAGCGTGCACTAGGACTTGATGAGCTTCATATGTATGATTTATATACACCACTTGTACCAGAAGTGAAAATG
AATGTGAAATACGAAGAAGCGCAAGACCTTTTATTAAAATCTTTAAACGTACTTGGTGATGAATATGTTGATATTTTGAA
AGAGGCATATGAAAATCGCTGGGTAGATGTGTATGAGAATAAAGGAAAACGAAGCGGGGCATATTCATCTGGTGCATATG
GAACAAATCCGTATATTTTAATGAACTGGCATGATAATGTAAATAATTTATTTACACTTGCTCATGAGTTTGGTCATTCG
GTGCATAGTTACTATACAAGAAAAACACAACCGCACGTATATGGTGATTATTCCATCTTCGTAGCAGAAGTGGCATCTAC
TTGTAATGAAGCACTTCTAAATGATTATTTATTAAAAACGACAGAAGATAAGAAAGAGCGTCTATATTTATTAAATCATT
ATTTAGAAGGGTTCCGTGGTACTGTATTCCGTCAAACGATGTTTGCAGAGTTTGAACATATTATTCATAAGAAAGTACAA
GAAGGACATGCTGTTACGCCAGACATGTTAACGGAAATTTACTATGATTTAAATAAGAAATATTTCGGTGACGCTTTAGT
AATCGACGAAGAGATTGGTTTAGAATGGTCTCGTATCCCACACTTCTACTACAACTATTACGTATATCAATACGCAACAG
GATTTAGTGCTGCGACAGCTCTATCTAAACAGATTTTAGAAGAAGGACAACCAGCAGTAGAACGTTATATTAATGAGTTC
TTAAAAGCAGGAAGCTCTGATTATCCAATTGAAGTGCTGAAAAAAGCAGGAGTAGATATGGCATCTCCTGAACCAGTAAA
AGAGGCGCTACAAGTATTTGAAGAGAAATTAAATGAATTAGAAGCATTATTATTTGAAGAAAAGTAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(211-591)

Oligopeptidase F (Peptidase_M3_N)

(120-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

50.084

97.533

0.488

Multiple sequence alignment