Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DT536_RS03130 Genome accession   NZ_CP031011
Coordinates   651686..653398 (+) Length   570 a.a.
NCBI ID   WP_114392504.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain LXL_C1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 646686..658398
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DT536_RS03090 (DT536_03090) nusA 647414..648898 (-) 1485 WP_004692706.1 transcription termination factor NusA -
  DT536_RS03095 (DT536_03095) rimP 648938..649462 (-) 525 WP_058868797.1 ribosome maturation factor RimP -
  DT536_RS03120 (DT536_03120) secG 650272..650601 (-) 330 WP_058868796.1 preprotein translocase subunit SecG -
  DT536_RS03125 (DT536_03125) tpiA 650615..651406 (-) 792 WP_058868795.1 triose-phosphate isomerase -
  DT536_RS03130 (DT536_03130) pilB 651686..653398 (+) 1713 WP_114392504.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DT536_RS03135 (DT536_03135) pilC 653429..654655 (+) 1227 WP_010325767.1 type II secretion system F family protein Machinery gene
  DT536_RS03140 (DT536_03140) pilD 654655..655515 (+) 861 WP_058868793.1 prepilin peptidase Machinery gene
  DT536_RS03145 (DT536_03145) coaE 655524..656123 (+) 600 WP_058868792.1 dephospho-CoA kinase -
  DT536_RS03150 (DT536_03150) - 656137..657054 (-) 918 WP_058868791.1 DMT family transporter -
  DT536_RS03155 (DT536_03155) rlmB 657087..657836 (-) 750 WP_004692717.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  DT536_RS03160 (DT536_03160) - 657979..658302 (-) 324 WP_004692718.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63617.92 Da        Isoelectric Point: 4.9493

>NTDB_id=302821 DT536_RS03130 WP_114392504.1 651686..653398(+) (pilB) [Acinetobacter johnsonii strain LXL_C1]
MSVSNGLPKFTGFIRRLVEDGRVTAENMQQAMLSAKKAQQDIVPYLIENYKLSPQMIAETISLEFGEPVFDLSVYDSVQI
LRDLVEDKLITKHRVLPIYRRGNILYIATSNPTNMDAMDAIRFNSKMNIEAIIVEHPQLEKLIEQNFAQADSFEFSDEDI
DLDLEQDHQNSNDEEDDSPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRHIASPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKDLFMQALDKP
QGMLLITGPTGSGKTVSLYTGLNILNKEDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKAAVDIPEQSLLEMGF
TSTDIKDPDFKVYQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAEASAKLGFNNLRRSGLLKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=302821 DT536_RS03130 WP_114392504.1 651686..653398(+) (pilB) [Acinetobacter johnsonii strain LXL_C1]
ATGTCAGTATCAAATGGTTTGCCTAAGTTCACAGGCTTCATTCGACGCTTAGTCGAAGACGGACGCGTGACTGCTGAAAA
TATGCAGCAGGCAATGCTCTCTGCAAAAAAGGCTCAGCAAGACATCGTTCCTTATCTAATTGAAAATTATAAACTCTCCC
CACAAATGATCGCTGAGACGATTTCTTTGGAGTTTGGCGAGCCTGTTTTTGACTTAAGTGTCTATGATTCGGTACAAATT
CTACGGGATCTGGTCGAAGACAAACTCATCACCAAGCATCGTGTGTTGCCGATTTACCGTCGTGGCAATATCCTCTATAT
CGCCACCAGCAATCCAACCAACATGGATGCAATGGACGCGATTCGTTTCAATTCTAAAATGAACATTGAAGCGATTATTG
TTGAACATCCTCAGCTTGAAAAACTAATCGAACAAAATTTCGCGCAAGCCGATAGCTTTGAATTTTCTGATGAAGATATT
GATTTAGACTTAGAGCAAGACCATCAGAACTCCAATGACGAAGAAGATGACTCGCCTCAAGGGGATGAAGCGCCGATCGT
CAAATACATCAATAAATTACTGATCGATGCGATTCGTATGGGCGCATCAGACTTGCACTTTGAACCTTATGAAAAATCTT
ACCGTGTCCGTTATCGAGTAGATGGGGTTCTGCGTCATATTGCCAGTCCTCCACTACAACTGGCGACTCGTCTTGCCTCA
CGTTTAAAAGTTATGTCACAAATGGACATCTCTGAAAAGCGCGTCCCGCAAGATGGCCGGATTAAATTAAAGCTGTCGAA
AAGTAAAGCCATTGACTTTCGTGTCAACTCACTTCCAACCTTGTTTGGTGAAAAACTGGTTCTGCGTATTCTCGATCCAT
CCAGTGCTATGCTGGGGATTGATGCTTTAGGTTATGAACCAGATCAAAAAGACCTATTTATGCAGGCTTTAGACAAACCG
CAAGGCATGTTGTTAATTACTGGACCGACAGGTTCAGGTAAAACGGTATCGCTGTATACAGGTTTGAATATTCTAAATAA
AGAAGACACCAATATCTCAACTGCAGAAGACCCCGTTGAGATTAACTTAGAAGGCATTAATCAGGTTAACGTGAACAATA
AAGTCGGTCTTACCTTCTCTGCTGCGCTGAAGTCATTCTTACGTCAAGATCCTGATATTGTAATGGTGGGTGAGATTCGT
GACCTTGAAACGGCTGAAATCGCGATTAAAGCCGCACAGACAGGCCACATGGTCATGTCGACACTCCACACCAATAGTGC
ACCTGAAACTCTGACACGCTTACGCAATATGGGGGTTCCATCTTTTAACATTGCGACATCGGTCAATTTGGTAATTGCAC
AGCGTTTGGCACGCCGTTTATGTAGCCAATGCAAAGCCGCTGTCGATATTCCTGAACAAAGCTTGCTCGAAATGGGCTTT
ACTTCAACAGATATCAAAGATCCAGACTTCAAAGTTTATCAACCGGTGGGATGTACTGAGTGCCGTGAAGGCTATAAAGG
ACGTGTTGGTATTTATGAAGTCATGAAAGTCACACCAGAAATTTCAAAAATTATTATGGAAGATGGCAATGCCTTACAGA
TTGCGGAAGCATCTGCAAAATTAGGATTTAACAATTTACGTCGTTCAGGTTTACTCAAGGTTATGCAGGGGGTCACTTCC
TTACAAGAAGTGAATCGTGTAACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.404

100

0.814
  pilB Acinetobacter baylyi ADP1

76.842

100

0.768
  pilB Legionella pneumophila strain ERS1305867

53.933

93.684

0.505
  pilF Neisseria gonorrhoeae MS11

50

98.596

0.493
  pilB Vibrio cholerae strain A1552

48.677

99.474

0.484
  pilB Vibrio campbellii strain DS40M4

47.67

97.895

0.467
  pilB Vibrio parahaemolyticus RIMD 2210633

50.677

90.702

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

34.572

100

0.375
  pilF Thermus thermophilus HB27

36.602

100

0.367

Multiple sequence alignment