Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DT536_RS01230 Genome accession   NZ_CP031011
Coordinates   248913..250034 (+) Length   373 a.a.
NCBI ID   WP_004984301.1    Uniprot ID   N9BCS7
Organism   Acinetobacter johnsonii strain LXL_C1     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 243913..255034
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DT536_RS01215 (DT536_01215) - 246119..246853 (-) 735 WP_058869039.1 hypothetical protein -
  DT536_RS01220 (DT536_01220) - 247037..247726 (-) 690 WP_058869038.1 YggS family pyridoxal phosphate-dependent enzyme -
  DT536_RS01225 (DT536_01225) pilT 247849..248886 (+) 1038 WP_004694862.1 type IV pilus twitching motility protein PilT Machinery gene
  DT536_RS01230 (DT536_01230) pilU 248913..250034 (+) 1122 WP_004984301.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DT536_RS01235 (DT536_01235) fur 250094..250531 (-) 438 WP_004694866.1 ferric iron uptake transcriptional regulator -
  DT536_RS01240 (DT536_01240) - 250644..251042 (+) 399 WP_004694868.1 outer membrane protein assembly factor BamE -
  DT536_RS01245 (DT536_01245) - 251089..251412 (-) 324 WP_058869037.1 RnfH family protein -
  DT536_RS01250 (DT536_01250) - 251412..252494 (-) 1083 WP_058869036.1 hypothetical protein -
  DT536_RS01255 (DT536_01255) - 252637..253092 (-) 456 WP_005400160.1 bacteriohemerythrin -
  DT536_RS01260 (DT536_01260) - 253367..254215 (-) 849 WP_058869035.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41849.12 Da        Isoelectric Point: 6.6662

>NTDB_id=302815 DT536_RS01230 WP_004984301.1 248913..250034(+) (pilU) [Acinetobacter johnsonii strain LXL_C1]
MDFNDLLNYMIQQKASDLFITADVEPSMKINGQIVPVAKGKLTGDVAGQLLNSIMSDKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETQIPTMDELKLPPVLKELAMTKRGIIIFVGATGTGKSTSLASIIGYRNQNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHSQLFMDLSLNLKAMVAQQLIPTPDGNSRRAAIEILINSPLISDLIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAKQITYKDALKHADSPNDLRLQIKLSEEGGDRLLHASANITFDGQA

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=302815 DT536_RS01230 WP_004984301.1 248913..250034(+) (pilU) [Acinetobacter johnsonii strain LXL_C1]
ATGGATTTTAACGACTTACTCAATTATATGATTCAACAAAAAGCGTCGGATTTATTTATCACCGCTGATGTTGAACCCTC
CATGAAAATTAACGGTCAAATTGTGCCTGTGGCTAAGGGTAAGCTGACGGGTGATGTCGCGGGGCAACTGCTCAACTCGA
TTATGAGTGACAAGCAGCGTAAAGAATTTGCAGACACACGTGAGTGCAACTTCGCGATTATGAATCGGGATAAAACTGCA
CGTTTTCGTGTCAGTGCCTTTCAACAGCGTGATATGCCAGGTATGGTTTTGCGTCGAATTGAGACGCAAATTCCAACCAT
GGATGAGTTAAAACTCCCGCCTGTACTCAAAGAGTTGGCCATGACCAAACGTGGCATCATCATCTTTGTGGGTGCAACAG
GTACGGGTAAATCGACCTCTTTAGCTTCGATCATTGGTTACCGTAACCAAAACTCAAAAGGTCATATCATTACCATTGAA
GACCCAATTGAATTTATTCATGAGCATGCAGGCTGTATCATTACCCAGCGTGAAGTGGGCATTGATACGGACTCTTTTGA
AATCGCACTGAAAAACACTTTACGTCAGGCACCCGATGTAATTCTGATTGGTGAGATTCGTTCCCGTGAAACCATGGATT
ATGCGATTGCCTTTGCTGAAACAGGTCACTTGGTATTTGCAACGCTGCACGCCAACAACGCCAACCAAGCCATTGACCGT
ATTATTCACTTCTTTGAAGCAGACCGTCATAGCCAATTGTTTATGGACTTGTCTTTAAACCTTAAAGCGATGGTTGCTCA
GCAGTTAATTCCAACACCTGACGGTAATTCACGTCGCGCTGCAATTGAGATTTTGATTAACTCACCGTTAATTTCCGATT
TGATCCGTAAAGGTGAAATCCACGAAATTAAAGACCTGATGAAGCGTTCTCGTGAACTCGGTATGCAAACCTTTGACCAA
GCCTTATTCGATTTGTACAAAGCCAAGCAGATTACCTATAAAGATGCGCTGAAACACGCCGACTCTCCAAACGACTTACG
CTTACAAATCAAGTTGTCTGAAGAAGGCGGTGATCGTCTATTGCATGCCAGTGCAAACATCACGTTTGACGGACAAGCTT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9BCS7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.72

100

0.777
  pilU Pseudomonas stutzeri DSM 10701

67.925

99.464

0.676
  pilU Vibrio cholerae strain A1552

55.405

99.196

0.55
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.197

92.761

0.391
  pilT Legionella pneumophila strain Lp02

41.42

90.617

0.375
  pilT Legionella pneumophila strain ERS1305867

41.42

90.617

0.375
  pilT Pseudomonas aeruginosa PAK

39.306

92.761

0.365

Multiple sequence alignment