Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA/cilB/dalA   Type   Machinery gene
Locus tag   SPH_RS06685 Genome accession   NC_010380
Coordinates   1280994..1281842 (-) Length   282 a.a.
NCBI ID   WP_000705306.1    Uniprot ID   A0A098Z7D2
Organism   Streptococcus pneumoniae Hungary19A-6     
Function   ssDNA binding; loading RecA onto ssDNA; competence shut-off (predicted from homology)   
DNA processing Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1280821..1327266 1280994..1281842 within 0


Gene organization within MGE regions


Location: 1280821..1327266
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPH_RS06685 (SPH_1382) dprA/cilB/dalA 1280994..1281842 (-) 849 WP_000705306.1 DNA-processing protein DprA Machinery gene
  SPH_RS06690 (SPH_1383) - 1281948..1282637 (-) 690 WP_000643970.1 sugar phosphate nucleotidyltransferase -
  SPH_RS06695 (SPH_1384) - 1282649..1283527 (-) 879 WP_000837609.1 DMT family transporter -
  SPH_RS06700 (SPH_1385) licA 1283517..1284386 (-) 870 WP_000411204.1 choline kinase LicA -
  SPH_RS06705 (SPH_1386) - 1284403..1285425 (-) 1023 WP_000609894.1 ribitol-5-phosphate dehydrogenase -
  SPH_RS06710 (SPH_1387) - 1285430..1286137 (-) 708 WP_000638504.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  SPH_RS06715 (SPH_1388) tacF 1286472..1287959 (+) 1488 WP_000835896.1 type IV teichoic acid flippase TacF -
  SPH_RS06720 (SPH_1389) - 1287969..1288772 (+) 804 WP_000811992.1 phosphorylcholine transferase LicD -
  SPH_RS06725 (SPH_1390) - 1288884..1289582 (+) 699 WP_000900061.1 phosphorylcholine transferase LicD -
  SPH_RS06730 (SPH_1391) carB 1289857..1293033 (-) 3177 WP_012291583.1 carbamoyl-phosphate synthase large subunit -
  SPH_RS06735 (SPH_1392) - 1293346..1294425 (-) 1080 WP_000166701.1 carbamoyl phosphate synthase small subunit -
  SPH_RS06740 (SPH_1393) - 1294475..1295398 (-) 924 WP_001293845.1 aspartate carbamoyltransferase catalytic subunit -
  SPH_RS06745 (SPH_1394) pyrR 1295417..1295938 (-) 522 WP_000850024.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  SPH_RS06750 (SPH_1395) nth 1296149..1296778 (-) 630 WP_000244451.1 endonuclease III -
  SPH_RS06755 (SPH_1396) - 1296778..1297320 (-) 543 WP_000773226.1 DUF177 domain-containing protein -
  SPH_RS06760 (SPH_1397) - 1297490..1297807 (+) 318 WP_000389597.1 hypothetical protein -
  SPH_RS06765 (SPH_1398) - 1298046..1299605 (+) 1560 WP_001812708.1 ATP-binding cassette domain-containing protein -
  SPH_RS06770 (SPH_1399) htpX 1299754..1300653 (-) 900 WP_000895736.1 zinc metalloprotease HtpX -
  SPH_RS06775 (SPH_1400) - 1300655..1301215 (-) 561 WP_000219823.1 LemA family protein -
  SPH_RS06780 (SPH_1401) rsmG 1301309..1302022 (+) 714 WP_000801941.1 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG -
  SPH_RS06785 (SPH_1402) - 1302209..1303426 (-) 1218 WP_001291561.1 tyrosine-type recombinase/integrase -
  SPH_RS06790 (SPH_1403) - 1303508..1303711 (-) 204 WP_000814511.1 excisionase -
  SPH_RS13990 (SPH_1404) - 1303695..1303946 (+) 252 WP_001845478.1 hypothetical protein -
  SPH_RS06795 (SPH_1405) - 1304172..1304402 (-) 231 WP_000857133.1 helix-turn-helix domain-containing protein -
  SPH_RS06800 (SPH_1406) - 1304399..1304821 (-) 423 WP_000804885.1 sigma-70 family RNA polymerase sigma factor -
  SPH_RS06805 (SPH_1408) - 1305326..1305679 (+) 354 WP_001227347.1 helix-turn-helix transcriptional regulator -
  SPH_RS12650 - 1305739..1305906 (-) 168 WP_000336323.1 cysteine-rich KTR domain-containing protein -
  SPH_RS06815 (SPH_1409) tet(M) 1306025..1307944 (-) 1920 WP_000691747.1 tetracycline resistance ribosomal protection protein Tet(M) -
  SPH_RS12655 (SPH_1410) - 1307960..1308076 (-) 117 WP_001814923.1 tetracycline resistance determinant leader peptide -
  SPH_RS06820 (SPH_1411) - 1308321..1309256 (-) 936 WP_001224320.1 conjugal transfer protein -
  SPH_RS06825 (SPH_1412) - 1309253..1310254 (-) 1002 WP_000769864.1 bifunctional lysozyme/C40 family peptidase -
  SPH_RS06830 (SPH_1413) - 1310251..1312428 (-) 2178 WP_012291584.1 membrane protein -
  SPH_RS06835 (SPH_1414) - 1312431..1314878 (-) 2448 WP_000331161.1 ATP-binding protein -
  SPH_RS06840 (SPH_1415) - 1314862..1315254 (-) 393 WP_000723888.1 conjugal transfer protein -
  SPH_RS06845 (SPH_1416) - 1315343..1315840 (-) 498 WP_000342539.1 antirestriction protein ArdA -
  SPH_RS06850 (SPH_1417) - 1315957..1316178 (-) 222 WP_001009056.1 hypothetical protein -
  SPH_RS06855 (SPH_1418) - 1316358..1317605 (+) 1248 WP_000019067.1 ISL3 family transposase -
  SPH_RS06860 (SPH_1420) erm(B) 1317862..1318599 (-) 738 WP_001038795.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
  SPH_RS06865 - 1318724..1318807 (-) 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
  SPH_RS06870 (SPH_1421) - 1318856..1319065 (-) 210 WP_029658041.1 peptide-binding protein -
  SPH_RS06875 (SPH_1422) mobT 1319046..1320275 (-) 1230 WP_012291622.1 MobT family relaxase -
  SPH_RS12660 - 1320298..1320450 (-) 153 WP_000879507.1 hypothetical protein -
  SPH_RS06880 (SPH_1423) - 1320453..1321838 (-) 1386 WP_000813488.1 FtsK/SpoIIIE domain-containing protein -
  SPH_RS06885 (SPH_1424) - 1321867..1322253 (-) 387 WP_000985015.1 YdcP family protein -
  SPH_RS06890 (SPH_1425) - 1322269..1322583 (-) 315 WP_000420682.1 YdcP family protein -
  SPH_RS06895 (SPH_1427) - 1323215..1324498 (+) 1284 WP_012291612.1 solute carrier family 23 protein -
  SPH_RS06900 (SPH_1428) ffh 1324594..1326165 (-) 1572 WP_000863651.1 signal recognition particle protein -
  SPH_RS06905 (SPH_1429) - 1326177..1326509 (-) 333 WP_000402071.1 putative DNA-binding protein -
  SPH_RS06910 (SPH_1430) - 1326600..1326977 (-) 378 WP_000702355.1 DUF1934 domain-containing protein -

Sequence

Protein


Download         Length: 282 a.a.        Molecular weight: 31062.72 Da        Isoelectric Point: 5.4047

>NTDB_id=30014 SPH_RS06685 WP_000705306.1 1280994..1281842(-) (dprA/cilB/dalA) [Streptococcus pneumoniae Hungary19A-6]
MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADISGCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDC
YPWDLSEIYDAPVLLFYKGNLDLLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRGVIVAEAKMRSGSLITCERAMEE
GRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDVLAEFEF

Nucleotide


Download         Length: 849 bp        

>NTDB_id=30014 SPH_RS06685 WP_000705306.1 1280994..1281842(-) (dprA/cilB/dalA) [Streptococcus pneumoniae Hungary19A-6]
ATGAAAATCACAAACTATGAAATCTATAAGTTAAAAAAATCAGGTTTGACCAATCAACAGATTTTGAAAGTGCTAGAATA
CGGTGAAAATGTTGATCAGGAGCTTTTGTTGGGTGATATTGCAGATATATCAGGTTGCCGTAATCCAGCCGTTTTTATGG
AACGTTATTTTCAGATAGACGATGCGCATTTGTCGAAAGAGTTTCAAAAATTTCCATCTTTCTCTATTTTAGATGACTGT
TATCCTTGGGATTTGAGTGAAATATATGATGCGCCTGTACTTTTATTTTACAAGGGAAATCTTGACCTCCTGAAATTCCC
GAAGGTAGCGGTCGTGGGCAGTCGTGCTTGTAGCAAACAGGGAGCTAAGTCAGTTGAAAAAGTCATTCAAGGCTTGGAAA
ATGAACTGGTTATTGTCAGTGGTCTGGCCAAGGGCATTGACACAGCAGCTCATATGGCAGCTCTTCAGAATGGCGGAAAA
ACCATTGCAGTGATTGGAACAGGACTGGATGTGTTTTATCCTAAAGCCAATAAACGCTTGCAAGACTACATCGGCAATGA
CCATCTGGTTCTAAGTGAATATGGACCTGGCGAACAACCTCTGAAATTTCATTTTCCTGCCCGTAATCGCATCATTGCTG
GACTTTGTCGTGGTGTGATTGTAGCAGAGGCTAAGATGCGTTCAGGTAGTCTCATTACGTGTGAGCGAGCAATGGAAGAA
GGACGCGATGTCTTTGCTATTCCTGGTAGCATTTTAGATGGACTATCAGACGGTTGCCATCATTTGATTCAAGAAGGAGC
AAAATTGGTCACCAGTGGGCAAGATGTTCTTGCGGAATTTGAATTTTAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(75-271)

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(3-64)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A098Z7D2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

100

100

1
  dprA/cilB/dalA Streptococcus pneumoniae D39

100

100

1
  dprA/cilB/dalA Streptococcus pneumoniae R6

100

100

1
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

100

100

1
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

98.582

100

0.986
  dprA/cilB/dalA Streptococcus mitis SK321

97.872

100

0.979
  dprA Streptococcus mutans UA159

63.214

99.291

0.628
  dprA Lactococcus lactis subsp. cremoris KW2

55.714

99.291

0.553
  dprA Staphylococcus aureus N315

37.809

100

0.379
  dprA Haemophilus influenzae Rd KW20

38.202

94.681

0.362

Multiple sequence alignment