Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DM558_RS02340 Genome accession   NZ_CP029822
Coordinates   476052..477719 (-) Length   555 a.a.
NCBI ID   WP_127161877.1    Uniprot ID   A0A3Q9JHL4
Organism   Entomomonas moraniae strain QZS01     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 471052..482719
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DM558_RS02320 (DM558_02330) yacG 472943..473161 (-) 219 WP_127161873.1 DNA gyrase inhibitor YacG -
  DM558_RS02325 (DM558_02335) coaE 473154..473768 (-) 615 WP_127161874.1 dephospho-CoA kinase -
  DM558_RS02330 (DM558_02340) - 473820..474686 (-) 867 WP_127161875.1 A24 family peptidase -
  DM558_RS02335 (DM558_02345) pilC 474691..475899 (-) 1209 WP_127161876.1 type II secretion system F family protein Machinery gene
  DM558_RS02340 (DM558_02350) pilB 476052..477719 (-) 1668 WP_127161877.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DM558_RS02345 (DM558_02355) - 477896..479413 (-) 1518 WP_127161878.1 fumarate hydratase -
  DM558_RS02350 (DM558_02360) - 479814..482045 (+) 2232 WP_228411792.1 TonB-dependent siderophore receptor -
  DM558_RS02355 (DM558_02365) - 482049..482339 (+) 291 WP_109703230.1 iron transporter -

Sequence

Protein


Download         Length: 555 a.a.        Molecular weight: 61251.36 Da        Isoelectric Point: 6.5165

>NTDB_id=296197 DM558_RS02340 WP_127161877.1 476052..477719(-) (pilB) [Entomomonas moraniae strain QZS01]
MNASPQALTGLAKIFADSNLLSESDLHKAVEQAKVEHLSLVNYLVQNKKASAKALAAIISERFGLPLFDLSSLSIDTALR
TIITEKLARKHRVIPLWRRGNNLFIGISDPANQQGLKDIQFNTGLPLDAILIEDDKLTSLLDRFFDEGAEQVTTTNLTKT
MAAGDEEKEDESIVNFVNQMLLMAIRSGASDLHFEPYEKSYRVRFRTDGILQIKSRPPLELASRISARLKVMSNMDISER
RKPQDGRIKLKVSERKSIDFRVNSCPTLWGEKIVLRILDSSSAQLGIDQLGYEPDQKQLYLDALGHPQGMILVTGPTGSG
KTVSLYTGLNILNTPDINISTAEDPVEINLEGINQVNVNPRQGLDFSSALRAFLRQDPDIIMVGEIRDLETAEIAIKAAQ
TGHMVMSTLHTNSAPETLTRLMNMGVAAFNLATSVNLIIAQRLARRLCGHCKEELELPKEVLIKAGFPPEKIGTFSVFGP
KGCDRCNNGYKGRVGIYEVVKITPALQRIIMAEGNSIEIADEARKEGFDDLRTSGLKKVMAGLTSLEEVNRVTSD

Nucleotide


Download         Length: 1668 bp        

>NTDB_id=296197 DM558_RS02340 WP_127161877.1 476052..477719(-) (pilB) [Entomomonas moraniae strain QZS01]
ATGAATGCATCTCCTCAAGCCCTCACTGGGCTTGCAAAAATATTTGCTGACAGCAATCTTTTATCAGAAAGCGATCTCCA
TAAAGCTGTGGAGCAAGCAAAAGTAGAACATTTATCGCTGGTTAATTATCTCGTTCAAAACAAAAAAGCGAGCGCAAAAG
CGTTAGCCGCAATTATTTCTGAACGGTTTGGTTTACCTCTTTTTGACTTATCAAGCCTATCCATTGATACAGCTTTAAGA
ACAATTATTACAGAGAAGCTAGCCAGAAAACACCGTGTTATCCCTTTATGGAGACGAGGCAATAACCTATTCATTGGTAT
TTCAGACCCTGCCAATCAACAAGGTCTCAAAGATATTCAGTTTAATACTGGATTACCTCTCGATGCAATTTTAATTGAAG
ACGACAAACTGACCTCGTTGCTTGATCGTTTTTTTGATGAGGGTGCCGAGCAAGTTACTACCACCAACTTAACAAAAACG
ATGGCGGCAGGTGATGAGGAAAAAGAAGATGAATCAATTGTTAACTTCGTCAATCAAATGCTTCTTATGGCTATTCGTTC
AGGCGCGTCTGACTTACATTTCGAGCCCTATGAAAAAAGTTATCGAGTCCGTTTTCGTACAGATGGTATTTTACAAATAA
AATCACGTCCACCTTTAGAACTTGCTTCACGTATTTCTGCGCGCCTAAAGGTCATGTCAAATATGGATATTTCTGAGCGT
AGAAAACCTCAAGATGGACGTATTAAATTAAAAGTTTCAGAAAGAAAATCTATCGATTTTCGGGTTAACTCATGCCCTAC
CCTATGGGGTGAAAAGATTGTATTACGTATTTTAGACTCTTCCAGTGCCCAATTAGGAATTGATCAGCTTGGTTATGAAC
CTGATCAAAAACAACTTTATTTGGATGCTTTAGGTCATCCTCAAGGAATGATTCTAGTAACTGGCCCCACAGGATCAGGT
AAAACGGTATCTCTTTATACAGGTCTTAATATTTTAAATACTCCTGATATCAATATTTCAACCGCTGAAGACCCTGTAGA
AATCAACCTTGAAGGGATCAACCAAGTTAACGTTAATCCTCGACAGGGTTTAGACTTCTCCTCAGCACTCCGGGCATTCT
TACGTCAAGACCCTGACATTATCATGGTGGGGGAAATTCGTGACCTTGAAACAGCAGAAATTGCTATTAAAGCAGCACAA
ACAGGTCATATGGTAATGTCTACGCTGCATACCAACAGTGCTCCTGAAACATTAACACGTTTAATGAATATGGGGGTCGC
AGCCTTTAACTTAGCAACGTCTGTAAACCTTATCATTGCTCAGCGGTTAGCTAGAAGACTGTGTGGTCATTGTAAAGAAG
AGTTAGAGCTCCCTAAAGAAGTATTAATAAAAGCAGGATTTCCTCCTGAGAAAATAGGTACTTTTAGCGTGTTCGGCCCC
AAAGGCTGCGATCGCTGTAATAATGGCTATAAGGGTCGTGTAGGTATTTATGAGGTCGTTAAAATAACACCTGCATTACA
ACGTATTATTATGGCAGAGGGCAACTCTATCGAGATTGCCGATGAAGCAAGAAAAGAAGGGTTTGATGACTTACGCACAT
CAGGACTCAAAAAAGTTATGGCAGGCTTAACGAGTCTTGAGGAAGTCAATCGAGTGACATCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(179-446)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q9JHL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.053

100

0.591
  pilB Acinetobacter baumannii D1279779

57.746

100

0.591
  pilB Legionella pneumophila strain ERS1305867

51.937

100

0.532
  pilB Vibrio parahaemolyticus RIMD 2210633

48.759

100

0.495
  pilB Vibrio campbellii strain DS40M4

48.577

100

0.492
  pilB Vibrio cholerae strain A1552

48.75

100

0.492
  pilF Neisseria gonorrhoeae MS11

48.837

100

0.492
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.241

99.64

0.391
  pilB/pilB1 Synechocystis sp. PCC 6803

37.838

100

0.378
  pilF Thermus thermophilus HB27

37.251

99.64

0.371

Multiple sequence alignment