Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   DM292_RS15910 Genome accession   NZ_CP029772
Coordinates   3387071..3389173 (-) Length   700 a.a.
NCBI ID   WP_110778196.1    Uniprot ID   -
Organism   Stutzerimonas frequens strain R2A2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3382071..3394173
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DM292_RS15895 (DM292_15895) - 3383824..3385383 (-) 1560 WP_110778194.1 AAA family ATPase -
  DM292_RS15900 (DM292_15900) aroB 3385394..3386497 (-) 1104 WP_110778195.1 3-dehydroquinate synthase -
  DM292_RS15905 (DM292_15905) aroK 3386520..3387065 (-) 546 WP_063539617.1 shikimate kinase AroK -
  DM292_RS15910 (DM292_15910) pilQ 3387071..3389173 (-) 2103 WP_110778196.1 type IV pilus secretin PilQ Machinery gene
  DM292_RS15915 (DM292_15915) pilP 3389227..3389753 (-) 527 Protein_3132 type 4a pilus biogenesis lipoprotein PilP -
  DM292_RS15920 (DM292_15920) pilO 3389750..3390373 (-) 624 WP_110778197.1 type 4a pilus biogenesis protein PilO -
  DM292_RS15925 (DM292_15925) - 3390370..3390942 (-) 573 WP_063539613.1 PilN domain-containing protein -
  DM292_RS15930 (DM292_15930) comM 3390942..3392006 (-) 1065 WP_110778198.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 700 a.a.        Molecular weight: 75318.23 Da        Isoelectric Point: 4.7734

>NTDB_id=295992 DM292_RS15910 WP_110778196.1 3387071..3389173(-) (pilQ) [Stutzerimonas frequens strain R2A2]
MNSTLSRLGISVLVAFVSPAVLAANLNALDVAALPGDRVELKLAFDEPVAPPRGYTLEQPARIALDLPGVSNNLGAKNRE
LGVGNARSVTVVEAQGRTRLIVNLTSLVPYSTRVDGKNLFVVLGEHAGVSGPAAVSAQPSAVAQPSVTTAPAKAVAVGRA
IDNIDFRRGDDGAGNVIITLSDPSISSTIEEQGGKIRVNFAKAQLPESLRVRLDVQDFATPVRFVDSASQAGGASIAIEP
TGRYDYLAYQTDNKLTISVKPLTEDEAEKRKADRFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVSGNITLRL
QNVPWDQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELESQRQIAELAPLRREVVQVNYAKAADIARLFQSVTDN
GGMAEERGSIAVDDRTNNIIAYQTQERLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGNLGAGDKWSAWG
KNGNLDVEDEEGGGQRITWPMNTPFVDMGAVGSTSGIGLGFVTDNAILDLQLSAMEKTGNGEVVSQPKVVTADKETAKIL
KGSEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDEPDFSQAATTGVPAIRKNEVNAKVLVTDGETI
VIGGVFSNTQSKSVDKVPFLGDLPFLGRLFRRDLVQDRKSELLVFITPRIMNNQAIQASR

Nucleotide


Download         Length: 2103 bp        

>NTDB_id=295992 DM292_RS15910 WP_110778196.1 3387071..3389173(-) (pilQ) [Stutzerimonas frequens strain R2A2]
ATGAACAGTACTCTCTCGCGTCTCGGCATTTCAGTGCTTGTGGCGTTCGTCTCGCCGGCTGTACTGGCAGCCAATCTCAA
TGCCTTGGATGTGGCCGCTCTGCCGGGCGATCGAGTCGAACTCAAGCTGGCTTTCGACGAGCCCGTTGCGCCGCCGCGTG
GTTACACCCTTGAGCAGCCTGCTCGTATTGCCCTGGATCTGCCGGGTGTATCGAACAACCTGGGGGCGAAGAATCGTGAG
CTCGGGGTTGGTAATGCGCGTAGCGTTACGGTCGTTGAGGCGCAAGGACGTACTCGGTTGATCGTGAACCTCACTTCGCT
GGTGCCCTATTCCACTCGAGTGGATGGCAAGAATCTGTTCGTCGTACTGGGCGAACATGCCGGAGTGAGTGGCCCGGCAG
CGGTCTCAGCGCAGCCGAGCGCAGTGGCGCAGCCATCTGTTACGACGGCTCCCGCGAAGGCGGTGGCTGTTGGTAGGGCC
ATTGACAACATTGACTTTCGGCGTGGCGATGATGGAGCCGGTAATGTGATTATTACGCTATCCGATCCATCAATCAGCTC
GACCATTGAGGAGCAGGGCGGAAAAATTCGCGTAAATTTCGCCAAGGCTCAGTTGCCGGAATCGCTGCGTGTGCGCCTGG
ATGTCCAAGACTTCGCTACGCCTGTCAGGTTCGTTGATTCCGCTTCTCAGGCAGGTGGTGCGAGCATCGCAATCGAGCCG
ACCGGGCGATATGACTATTTGGCCTACCAGACCGACAACAAGCTGACTATCAGCGTAAAACCTCTTACGGAAGACGAAGC
GGAAAAGCGTAAGGCTGATCGATTTGCTTATTCTGGCGAGAAGCTGTCGCTTAATTTCCAAGACATCGACGTTCGCTCGG
TATTGCAGCTAATCGCTGATTTCACGGATCTAAATCTGGTCGCAAGCGATACCGTTAGCGGCAATATCACGCTGCGTCTG
CAGAACGTGCCGTGGGATCAGGCTCTGGACTTGGTGCTTAAGACCAAGGGGTTAGACAAGCGCCAGGTCGGTAACGTGCT
GCTCGTGGCTCCTGCCGACGAAATTGCCGCACGTGAACGTCAGGAGCTTGAGTCTCAGCGGCAGATTGCCGAACTGGCTC
CGCTTCGCCGTGAGGTGGTGCAGGTTAACTATGCCAAGGCCGCTGACATAGCCAGGCTATTTCAGTCGGTAACGGATAAT
GGCGGGATGGCGGAAGAGCGGGGATCTATTGCGGTTGATGACCGCACCAACAATATCATTGCTTACCAGACTCAGGAGCG
TCTCGATGAGTTGCGTCGTATCGTGGCTCAACTGGATATTCCGGTTCGGCAGGTAATGATTGAGGCGCGCATAGTTGAGG
CAAACGTCGACTACGATAAGAGCCTTGGTGTTCGTTGGGGCGGCAATCTCGGCGCTGGTGATAAGTGGAGTGCCTGGGGT
AAGAACGGCAATCTTGATGTTGAAGATGAGGAAGGGGGCGGTCAGCGAATCACCTGGCCAATGAATACGCCATTTGTCGA
CATGGGCGCGGTAGGCTCAACATCGGGCATTGGGCTTGGATTCGTGACCGATAACGCGATCTTGGATCTACAGCTATCCG
CAATGGAAAAAACCGGCAACGGCGAGGTTGTCTCGCAGCCTAAGGTAGTCACCGCAGACAAAGAAACTGCAAAGATCCTC
AAGGGCTCCGAAGTTCCTTATCAGGAGGCGAGCTCCAGTGGTGCAACGAGCACGTCGTTCAAGGAGGCAGCTCTCTCTCT
GGAAGTGACTCCGCAGATCACACCGGATAATCGCATCATCATGGAGGTCAAGGTCACTAAGGATGAGCCAGACTTCTCTC
AGGCAGCGACTACCGGAGTACCGGCGATTCGCAAGAATGAGGTTAACGCAAAGGTTCTGGTAACTGATGGTGAGACGATC
GTGATCGGTGGCGTTTTCTCCAATACGCAGAGCAAGTCAGTGGATAAGGTGCCGTTCCTAGGGGATCTCCCGTTCTTGGG
TCGCCTGTTCCGTCGTGACCTGGTGCAGGATCGAAAGTCTGAACTGCTTGTGTTCATCACGCCGAGGATCATGAATAATC
AGGCGATTCAAGCCAGTCGCTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(305-351)

AMIN domain (AMIN)

(38-123)

Bacterial type II/III secretion system short domain (Secretin_N)

(378-444)

Bacterial type II and III secretion system protein (Secretin)

(534-691)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

74.131

100

0.761
  pilQ Acinetobacter baumannii strain A118

39.054

100

0.413
  pilQ Acinetobacter baumannii D1279779

39.054

100

0.413
  comQ Acinetobacter baylyi ADP1

41.026

100

0.411
  pilQ Legionella pneumophila strain ERS1305867

37.865

97.714

0.37
  pilQ Legionella pneumophila strain Lp02

37.573

97.714

0.367

Multiple sequence alignment