Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   DKY63_RS21735 Genome accession   NZ_CP029693
Coordinates   4631337..4633427 (+) Length   696 a.a.
NCBI ID   WP_110965968.1    Uniprot ID   A0A2Z4RMT7
Organism   Pseudomonas putida strain JBC17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4626337..4638427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DKY63_RS21715 (DKY63_21715) comM 4628552..4629613 (+) 1062 WP_110965964.1 pilus assembly protein PilM Machinery gene
  DKY63_RS21720 (DKY63_21720) - 4629613..4630179 (+) 567 WP_110965965.1 PilN domain-containing protein -
  DKY63_RS21725 (DKY63_21725) pilO 4630176..4630799 (+) 624 WP_110965966.1 type 4a pilus biogenesis protein PilO -
  DKY63_RS21730 (DKY63_21730) - 4630796..4631323 (+) 528 WP_110965967.1 pilus assembly protein PilP -
  DKY63_RS21735 (DKY63_21735) pilQ 4631337..4633427 (+) 2091 WP_110965968.1 type IV pilus secretin PilQ Machinery gene
  DKY63_RS21740 (DKY63_21740) aroK 4633432..4633950 (+) 519 WP_056725695.1 shikimate kinase AroK -
  DKY63_RS21745 (DKY63_21745) aroB 4634102..4635202 (+) 1101 WP_110965969.1 3-dehydroquinate synthase -
  DKY63_RS21750 (DKY63_21750) - 4635213..4636813 (+) 1601 Protein_4180 AAA family ATPase -

Sequence

Protein


Download         Length: 696 a.a.        Molecular weight: 75282.48 Da        Isoelectric Point: 5.2966

>NTDB_id=295388 DKY63_RS21735 WP_110965968.1 4631337..4633427(+) (pilQ) [Pseudomonas putida strain JBC17]
MNRTFSTLGMSLWIALLSPMVQAANLKALDVAALPGDRVELKLAFDQPPPAPRGYTTESPARIALDLPGVASQLASKTRD
LGSGNARSATVVETTDRTRLIINLTQLSPYSTRVEGNNLFVEVGQGSKMAPAKAPASRAPAAAVSIPAPARQSVASSKAI
RGVDFQRGTQGEGNVVIDLSDPSIAPDIQEREGKIVLGFARTQLPEPLRVRLDVKDFATPVQFVNAAATGDRATITIEPS
GAFDYSTYQTDNKLTVSIRPMTVDDLQKRNADRYAYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQ
NVPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAE
AKVDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGV
SNGANGSTTIGTPGSTSTNSPFVDMGAAGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGT
EIPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDFLNKVQDVPPIKKNEVNAKVLVNDGETIVIGG
VFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAFAVSR

Nucleotide


Download         Length: 2091 bp        

>NTDB_id=295388 DKY63_RS21735 WP_110965968.1 4631337..4633427(+) (pilQ) [Pseudomonas putida strain JBC17]
ATGAACAGGACTTTCTCCACCCTCGGGATGTCGCTATGGATAGCGCTGCTTTCGCCGATGGTACAAGCGGCCAACCTCAA
GGCGCTGGATGTCGCCGCGCTGCCCGGAGACCGCGTCGAGTTGAAGCTGGCGTTCGATCAGCCACCGCCGGCGCCGCGGG
GCTACACCACGGAGTCGCCCGCGCGAATTGCGCTGGATTTGCCCGGCGTCGCCAGTCAATTGGCGAGCAAGACCCGTGAT
CTGGGCAGCGGCAATGCCCGGAGCGCGACGGTGGTCGAAACCACTGATCGTACGCGGCTGATCATCAACCTGACTCAATT
GAGTCCCTACAGCACGCGAGTCGAAGGAAACAACCTGTTCGTGGAAGTAGGCCAGGGAAGCAAGATGGCGCCTGCCAAGG
CCCCGGCTTCCAGGGCGCCAGCCGCTGCGGTGTCGATACCCGCCCCGGCGCGTCAATCCGTGGCGAGCTCCAAGGCGATT
CGCGGGGTGGATTTTCAGCGCGGCACCCAGGGCGAAGGCAACGTGGTGATCGATTTGTCGGACCCCTCCATTGCTCCGGA
CATTCAGGAGCGCGAAGGCAAGATCGTGCTCGGTTTCGCCAGGACCCAATTGCCCGAGCCTTTGCGTGTGCGCCTGGACG
TCAAGGATTTCGCCACCCCGGTGCAATTCGTCAATGCCGCCGCCACAGGTGACAGGGCCACGATCACCATCGAGCCCAGC
GGTGCATTCGATTACTCCACCTACCAGACCGACAACAAGTTGACCGTCAGCATCCGTCCGATGACCGTCGACGACCTGCA
AAAGCGCAACGCCGATCGATACGCCTACAGCGGCGAGAAGCTATCGCTGAATTTCCAGGACATCGATGTGCGTTCGGTGC
TGCAACTGATTGCCGACTTCACCAACCTCAATCTGGTGGCCAGCGATACGGTGCAAGGCGGGATCACGTTGCGTCTGCAA
AACGTGCCCTGGGATCAAGCGCTGGATCTGGTGCTGAAAACCAAGGGTCTGGACAAGCGCAAGATCGGCAACGTGTTGCT
GGTGGCGCCGGCCGATGAAATCGCCGCCCGGGAGCGCCAGGAACTGGAGTCGCAAAAGCAGATTGCTGAACTGGCGCCCT
TGCGGCGTGAGCTGTTGCAGGTCAACTACGCCAAGGCGGCCGATATCGCCAAGTTGTTCCAGTCGGTGACCAGCGCCGAG
GCGAAAGTTGACGAACGGGGTTCGATCACCGTGGATGAGCGGACCAACAACATCATCGCCTACCAGACCCAGGACCGGCT
CGATGAACTGCGCCGGATCGTGGCGCAACTGGATATTCCGGTGCGTCAGGTGATGATCGAGGCACGAATCGTCGAGGCCA
ACGTGGATTACGACAAGAGTCTCGGGGTGCGCTGGGGTGGTTCGATCCAGAACAAGGGCAACTGGAACACCTCTGGCGTC
AGCAACGGTGCCAATGGATCAACCACCATCGGCACGCCCGGCAGTACCAGCACCAACTCACCGTTCGTCGACATGGGGGC
CGCAGGAAACACGTCGGGAATCGGTATCGCGTTCATTACCGACAACGTTTTGCTCGATCTTGAACTGACGGCCATGGAGA
AGACCGGCAACGGCGAAATCGTCTCGCAACCCAAGGTGGTCACGTCCGACAAGGAAACCGCGAAGATCCTCAAGGGCACC
GAGATTCCCTATCAGGAAGCAAGCTCCAGCGGCGCGACATCCGTGTCTTTCAAGGAGGCGTCGCTGTCGCTGGAGGTCAC
GCCGCAGATCACACCGGACAACCGGATCATCATGGAAGTGAAGGTTACCAAGGACGAACCCGACTTCTTGAACAAGGTGC
AGGATGTACCGCCGATCAAGAAAAACGAGGTCAATGCCAAGGTCTTGGTCAATGATGGCGAGACCATCGTAATTGGCGGC
GTTTTCTCAAATACTCAAAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCG
GCGTGACGTGGTTTCGGAGAAAAAATCCGAGCTGTTGGTGTTTCTCACTCCGCGTATCATGAATAACCAGGCGTTTGCTG
TGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(304-350)

AMIN domain (AMIN)

(38-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(378-443)

Bacterial type II and III secretion system protein (Secretin)

(531-687)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2Z4RMT7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.184

100

0.753
  comQ Acinetobacter baylyi ADP1

41.439

99.856

0.414
  pilQ Acinetobacter baumannii D1279779

38.318

100

0.412
  pilQ Acinetobacter baumannii strain A118

38.318

100

0.412
  pilQ Legionella pneumophila strain Lp02

38.881

97.557

0.379
  pilQ Legionella pneumophila strain ERS1305867

38.815

96.983

0.376

Multiple sequence alignment