Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   DKY63_RS21715 Genome accession   NZ_CP029693
Coordinates   4628552..4629613 (+) Length   353 a.a.
NCBI ID   WP_110965964.1    Uniprot ID   A0A2Z4RN52
Organism   Pseudomonas putida strain JBC17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4623552..4634613
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DKY63_RS21705 (DKY63_21705) - 4624392..4625660 (+) 1269 WP_110965962.1 malic enzyme-like NAD(P)-binding protein -
  DKY63_RS21710 (DKY63_21710) - 4625893..4628337 (-) 2445 WP_110965963.1 penicillin-binding protein 1A -
  DKY63_RS21715 (DKY63_21715) comM 4628552..4629613 (+) 1062 WP_110965964.1 pilus assembly protein PilM Machinery gene
  DKY63_RS21720 (DKY63_21720) - 4629613..4630179 (+) 567 WP_110965965.1 PilN domain-containing protein -
  DKY63_RS21725 (DKY63_21725) pilO 4630176..4630799 (+) 624 WP_110965966.1 type 4a pilus biogenesis protein PilO -
  DKY63_RS21730 (DKY63_21730) - 4630796..4631323 (+) 528 WP_110965967.1 pilus assembly protein PilP -
  DKY63_RS21735 (DKY63_21735) pilQ 4631337..4633427 (+) 2091 WP_110965968.1 type IV pilus secretin PilQ Machinery gene
  DKY63_RS21740 (DKY63_21740) aroK 4633432..4633950 (+) 519 WP_056725695.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 37788.35 Da        Isoelectric Point: 4.7970

>NTDB_id=295387 DKY63_RS21715 WP_110965964.1 4628552..4629613(+) (comM) [Pseudomonas putida strain JBC17]
MPGLFNKKANTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPPSAVTEKNIAELEGVGQALARVVTKARTSLKNVAV
AVAGSAVITKTIEMDAGLSDDEMESQLKIEADQYIPYPLDEVAIDFEVQGASARNPERVNVLLAACRKENVEVREAALTL
AGLTARVVDVEAYALERSFGLLANHLMVAPERLVAVVDIGATMTTLSVLLGGRIIYTREQLFGGRQLTEEIQRRYGLTVE
QAGLAKKQGGLPSDYVSAVLQPFREALVQQVSRSLQFFFASGQYHAVDQIVLAGGTASVSGLDRLIEQQLGTPTLVANPF
ADMTLNGKVNAAALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=295387 DKY63_RS21715 WP_110965964.1 4628552..4629613(+) (comM) [Pseudomonas putida strain JBC17]
GTGCCAGGACTCTTCAACAAAAAGGCCAATACGCTTCTGGGTATCGACATCAGCTCAACTTCGGTGAAATTGCTGGAGTT
GAGCCGCCAGGGCGACCGCTATCGGGTCGAGGCTTACGCGGTCGAACCGCTGCCCCCCAGCGCGGTGACCGAGAAAAACA
TCGCCGAACTCGAAGGCGTGGGCCAGGCCCTCGCGCGCGTGGTGACCAAGGCCAGAACCAGCCTCAAGAACGTGGCGGTG
GCTGTCGCCGGCTCGGCGGTAATCACCAAGACCATCGAGATGGATGCCGGACTTTCCGATGACGAAATGGAAAGCCAACT
GAAGATCGAGGCCGATCAGTACATTCCCTATCCGCTGGATGAAGTCGCCATCGATTTCGAAGTCCAGGGTGCGTCCGCGC
GTAATCCCGAACGGGTCAACGTGCTGCTCGCCGCCTGCCGCAAGGAAAACGTCGAAGTGCGTGAGGCCGCCCTGACCCTG
GCTGGCCTCACGGCCCGAGTGGTCGATGTAGAGGCCTATGCCCTGGAGCGCTCCTTTGGCCTGCTGGCAAACCACCTGAT
GGTCGCCCCGGAGCGTTTGGTGGCGGTGGTCGACATTGGCGCGACCATGACGACTCTCAGCGTGCTGCTGGGTGGACGGA
TCATCTATACCCGCGAACAGTTGTTCGGTGGTCGCCAGTTAACCGAGGAGATTCAGCGTCGATATGGCCTGACTGTCGAG
CAGGCCGGGTTGGCCAAGAAGCAGGGCGGCTTGCCCAGCGACTACGTCAGCGCAGTCTTGCAACCCTTTCGCGAGGCGTT
GGTGCAGCAGGTCTCGCGTTCATTGCAGTTTTTCTTTGCTTCCGGTCAGTACCACGCCGTCGACCAAATTGTGCTGGCCG
GCGGCACGGCGTCGGTCTCCGGCCTGGATCGGCTGATCGAACAGCAATTGGGCACCCCGACCCTGGTGGCCAACCCGTTT
GCCGACATGACTCTCAATGGCAAGGTCAACGCCGCGGCGCTGGCCAGCGACGCCCCCGCCCTGATGATCGCCTGCGGGCT
GGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2Z4RN52

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

54

99.15

0.535
  pilM Acinetobacter baumannii D1279779

53.714

99.15

0.533
  comM Acinetobacter baylyi ADP1

52.691

100

0.527
  pilM Legionella pneumophila strain ERS1305867

47.74

100

0.479

Multiple sequence alignment