Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C1M51_RS11785 Genome accession   NZ_CP029606
Coordinates   2447110..2448828 (-) Length   572 a.a.
NCBI ID   WP_129450007.1    Uniprot ID   A0A4V0YN51
Organism   Methylibium sp. Pch-M     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2442110..2453828
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C1M51_RS11755 (C1M51_11680) - 2442936..2443295 (+) 360 WP_242500044.1 NUDIX domain-containing protein -
  C1M51_RS11760 (C1M51_11685) - 2443318..2443512 (-) 195 WP_129449997.1 DNA gyrase inhibitor YacG -
  C1M51_RS11765 (C1M51_11690) zapD 2443532..2444284 (-) 753 WP_129449999.1 cell division protein ZapD -
  C1M51_RS11770 (C1M51_11695) coaE 2444354..2444977 (-) 624 WP_129450001.1 dephospho-CoA kinase -
  C1M51_RS11775 (C1M51_11700) pilD 2444984..2445835 (-) 852 WP_129450003.1 A24 family peptidase Machinery gene
  C1M51_RS11780 (C1M51_11705) pilC 2445850..2447076 (-) 1227 WP_129450005.1 type II secretion system F family protein Machinery gene
  C1M51_RS11785 (C1M51_11710) pilB 2447110..2448828 (-) 1719 WP_129450007.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C1M51_RS11795 (C1M51_11720) ispB 2449091..2450020 (-) 930 WP_036229611.1 octaprenyl diphosphate synthase -
  C1M51_RS11800 (C1M51_11725) rplU 2450348..2450602 (+) 255 WP_129452175.1 50S ribosomal protein L21 -
  C1M51_RS11805 (C1M51_11730) rpmA 2450629..2450886 (+) 258 WP_011828112.1 50S ribosomal protein L27 -
  C1M51_RS11810 (C1M51_11735) cgtA 2450990..2452102 (+) 1113 WP_129450009.1 Obg family GTPase CgtA -
  C1M51_RS11815 (C1M51_11740) proB 2452139..2453257 (+) 1119 WP_129450011.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62328.47 Da        Isoelectric Point: 5.8320

>NTDB_id=294058 C1M51_RS11785 WP_129450007.1 2447110..2448828(-) (pilB) [Methylibium sp. Pch-M]
MDTLADAPQSSLSGVARVLVHAGKLNVKTAEDLVRSAKEQKRSFISAVLSAGAVNPSDLAHTLSSVLAMPLLDLAAVDPQ
RLPRNVIDAKLATQYQVLVLGKRGNRLFIAGADPTDQEAAERIKFATQLTPEWVIVEHDKLSRLVDAATTTASESLESLV
AGDFEFDVTEEDTSASEAADVTTDVEDAPVVRFLQKMLIDAINARASDLHFEPFEYNYRVRFRVDGELREITQPPIAIKD
KLASRIKVISRLDIAEKRVPQDGRMKLKFGSKAIDFRVSTLPTLFGEKIVIRILDPSSAKLGIEALGYEKVEKDRLLDAI
KRPYGMVLVTGPTGSGKTVSLYTCLNILNQPGVNISTVEDPAEINLPGVNQVNVNDKAGLNFSVALKAFLRQDPDVIMVG
EIRDLDTADIAIKAAQTGHMVMSTLHTNDAPTTLTRLMNMGVAPFNIASSVILITAQRLARKLCENCKAPADYPREALLR
AGFKEAELDGSWKPYRAVGCSSCSNGYRGRVGIYQVMPISEEIQRIILTQGNAVDIAKQAQQEGVRDLRQSGLVKVRAGV
TTLEEVISVTNE

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=294058 C1M51_RS11785 WP_129450007.1 2447110..2448828(-) (pilB) [Methylibium sp. Pch-M]
GTGGATACCCTTGCCGATGCCCCGCAGAGTTCGCTGTCCGGCGTCGCGCGCGTGCTCGTGCACGCGGGCAAGCTCAACGT
CAAGACCGCCGAAGATCTGGTCCGCAGCGCCAAGGAGCAGAAGCGCAGCTTCATCTCGGCGGTCCTGAGCGCCGGTGCCG
TCAACCCTTCGGATCTTGCCCACACGCTGTCCAGCGTGCTGGCGATGCCCTTGCTCGACTTGGCTGCGGTCGATCCGCAG
CGCCTGCCGCGCAACGTCATCGATGCCAAGCTGGCCACTCAGTATCAAGTATTGGTGCTGGGCAAGCGTGGCAACCGCCT
CTTCATCGCCGGGGCCGACCCTACCGACCAGGAAGCCGCTGAACGCATCAAGTTCGCCACGCAGCTGACGCCCGAGTGGG
TGATCGTCGAGCACGACAAGCTCTCCCGGCTCGTCGATGCCGCCACAACGACGGCCAGCGAATCCCTCGAGTCGCTGGTC
GCCGGTGATTTCGAATTCGACGTCACCGAGGAAGATACCAGCGCATCCGAGGCCGCCGACGTCACCACCGACGTCGAGGA
CGCGCCGGTCGTTCGTTTCCTGCAGAAGATGCTGATCGATGCGATCAACGCACGCGCCTCCGATCTGCACTTCGAGCCGT
TCGAATACAACTACCGCGTCCGCTTCCGCGTCGATGGCGAACTGCGCGAGATCACGCAGCCGCCGATTGCCATCAAGGAC
AAGCTCGCCTCCCGCATCAAGGTGATTTCCCGGCTCGACATTGCCGAGAAGCGTGTGCCGCAGGACGGCCGCATGAAGCT
GAAGTTCGGCAGCAAGGCGATCGATTTCCGGGTCAGCACCCTGCCCACCCTGTTCGGCGAGAAGATCGTGATCCGTATCC
TCGACCCGTCGAGCGCCAAGCTCGGTATCGAGGCCCTCGGCTACGAGAAGGTCGAGAAGGATCGCCTGCTCGACGCGATC
AAGCGGCCGTACGGCATGGTGCTGGTCACCGGCCCCACGGGCTCCGGCAAGACGGTGTCTCTGTACACCTGCCTTAACAT
CCTGAATCAGCCCGGCGTCAACATCTCCACCGTCGAGGACCCAGCCGAAATCAACCTCCCGGGCGTCAACCAGGTCAATG
TCAACGACAAGGCGGGGCTGAATTTCTCGGTCGCCCTGAAGGCCTTCCTGCGGCAGGATCCGGACGTCATCATGGTCGGC
GAAATTCGCGACCTCGACACGGCAGACATCGCCATCAAGGCCGCGCAGACCGGGCACATGGTGATGAGCACACTGCACAC
CAATGACGCGCCGACGACCCTGACGCGGCTGATGAACATGGGCGTCGCGCCGTTCAACATCGCCTCCAGCGTGATCCTGA
TCACGGCGCAGCGGCTGGCGCGCAAGCTCTGCGAGAACTGCAAGGCGCCGGCCGATTACCCTCGCGAGGCGCTGCTTCGT
GCAGGCTTCAAGGAAGCTGAACTCGATGGCAGCTGGAAACCGTATCGTGCCGTCGGCTGCTCCAGTTGCAGCAACGGCTA
CCGCGGGCGCGTCGGCATCTACCAGGTCATGCCGATCAGCGAAGAGATCCAGCGCATCATCCTGACGCAGGGCAATGCGG
TCGACATCGCCAAACAGGCACAGCAAGAGGGCGTGCGTGATCTGCGTCAATCCGGTCTGGTCAAGGTGCGAGCGGGCGTG
ACCACCCTGGAAGAAGTCATCTCGGTCACCAACGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4V0YN51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

52.962

100

0.531
  pilF Neisseria gonorrhoeae MS11

53.464

98.427

0.526
  pilB Acinetobacter baylyi ADP1

52.575

98.427

0.517
  pilB Legionella pneumophila strain ERS1305867

50.794

99.126

0.503
  pilB Vibrio parahaemolyticus RIMD 2210633

46.441

98.252

0.456
  pilB Vibrio campbellii strain DS40M4

46.085

98.252

0.453
  pilB Vibrio cholerae strain A1552

45.406

98.951

0.449
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.658

92.133

0.365
  pilF Thermus thermophilus HB27

36.46

98.776

0.36

Multiple sequence alignment