Detailed information NTG0294056

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: C1M51_RS11775
• Genome accession: NZ_CP029606
• Coordinates: 2444984..2445835 (-)
• Length: 283 a.a.
• NCBI ID: WP_129450003.1
• Uniprot ID: A0A4P6H0C2
• Organism: Methylibium sp. Pch-M
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2439984..2450835

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C1M51_RS11740 (C1M51_11665)	-	2440539..2441270 (+)	732	WP_129449991.1	pyrimidine 5'-nucleotidase	-
C1M51_RS11745 (C1M51_11670)	slmA	2441248..2441910 (+)	663	WP_011828101.1	nucleoid occlusion factor SlmA	-
C1M51_RS11750 (C1M51_11675)	-	2441962..2442873 (+)	912	WP_129449993.1	ATP-binding protein	-
C1M51_RS11755 (C1M51_11680)	-	2442936..2443295 (+)	360	WP_242500044.1	NUDIX domain-containing protein	-
C1M51_RS11760 (C1M51_11685)	-	2443318..2443512 (-)	195	WP_129449997.1	DNA gyrase inhibitor YacG	-
C1M51_RS11765 (C1M51_11690)	zapD	2443532..2444284 (-)	753	WP_129449999.1	cell division protein ZapD	-
C1M51_RS11770 (C1M51_11695)	coaE	2444354..2444977 (-)	624	WP_129450001.1	dephospho-CoA kinase	-
C1M51_RS11775 (C1M51_11700)	pilD	2444984..2445835 (-)	852	WP_129450003.1	A24 family peptidase	Machinery gene
C1M51_RS11780 (C1M51_11705)	pilC	2445850..2447076 (-)	1227	WP_129450005.1	type II secretion system F family protein	Machinery gene
C1M51_RS11785 (C1M51_11710)	pilB	2447110..2448828 (-)	1719	WP_129450007.1	type IV-A pilus assembly ATPase PilB	Machinery gene
C1M51_RS11795 (C1M51_11720)	ispB	2449091..2450020 (-)	930	WP_036229611.1	octaprenyl diphosphate synthase	-
C1M51_RS11800 (C1M51_11725)	rplU	2450348..2450602 (+)	255	WP_129452175.1	50S ribosomal protein L21	-

Sequence

Protein

• Length: 283 a.a.
• Molecular weight: 30386.15 Da
• Isoelectric Point: 8.4919

>NTDB_id=294056 C1M51_RS11775 WP_129450003.1 2444984..2445835(-) (pilD) [Methylibium sp. Pch-M]
MSSDLQGALLSPWALGLLGLCIGSFLNVVIHRLPQMMERAWRLESAELLGVAAPESPALTLAKPASRCPQCGHRIRWFEN
VPVLSWLLLRGRCSACKTPISPRYPLFEIATGLLFALLAWRFGAQPTVLAWCAFVAVLLALSGIDWDTTLLPDSLTLPLL
WAGLVAAALGWTLPLSTAVWGAVFGYLSLWSVYWLFKLTTGKEGMGAGDFKLLAALGAWLGWQMLLPIVLGASLIGAAVG
IAMKLSGALHEGRYVPFGPFLAGAGLVVLYAGSERVMGWLGWA

Nucleotide

• Length: 852 bp

>NTDB_id=294056 C1M51_RS11775 WP_129450003.1 2444984..2445835(-) (pilD) [Methylibium sp. Pch-M]
ATGTCGTCCGACCTGCAGGGCGCGCTGCTGTCGCCCTGGGCGCTGGGGCTGCTCGGGCTGTGCATCGGCAGCTTTCTCAA
TGTCGTCATCCATCGCCTGCCACAGATGATGGAGCGGGCCTGGCGACTCGAAAGCGCCGAGCTGCTCGGGGTCGCGGCCC
CAGAATCACCCGCGCTCACGCTGGCGAAACCCGCCTCGCGCTGCCCACAATGCGGTCATCGCATCCGCTGGTTCGAGAAT
GTCCCGGTGCTGAGCTGGCTGCTGTTGCGCGGGCGCTGCTCAGCCTGCAAGACACCGATCTCGCCGCGCTATCCTCTCTT
CGAGATCGCGACCGGGCTGTTGTTCGCCTTGCTGGCTTGGCGTTTCGGCGCGCAACCGACGGTGCTGGCGTGGTGCGCCT
TCGTCGCCGTCCTGCTGGCACTGTCCGGCATCGACTGGGACACGACGCTCCTGCCCGACAGCCTCACGTTACCGCTGCTC
TGGGCCGGGCTGGTGGCTGCCGCGCTCGGCTGGACGCTGCCGCTCTCGACGGCCGTCTGGGGCGCGGTGTTCGGCTACCT
GTCGCTGTGGTCGGTGTACTGGCTGTTCAAACTCACCACAGGCAAGGAAGGCATGGGCGCGGGCGACTTCAAGCTGCTGG
CCGCCCTCGGCGCCTGGCTCGGATGGCAGATGCTGCTGCCCATCGTGCTCGGGGCCTCCTTGATCGGTGCCGCCGTCGGC
ATCGCCATGAAGCTGAGCGGCGCTCTGCACGAAGGGCGCTACGTGCCCTTCGGCCCCTTCCTGGCCGGTGCCGGACTGGT
CGTCCTGTACGCAGGCTCCGAGCGTGTGATGGGCTGGCTCGGCTGGGCCTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-122)

• Type IV leader peptidase family (Peptidase_A24) (133-241)

3D structure

• AlphaFold DB: A0A4P6H0C2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.933	94.346	0.509
pilD	Vibrio campbellii strain DS40M4	51.685	94.346	0.488
pilD	Neisseria gonorrhoeae MS11	47.535	100	0.477
pilD	Acinetobacter baumannii D1279779	48.708	95.76	0.466
pilD	Acinetobacter nosocomialis M2	48.339	95.76	0.463