Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   SPEA_RS02225 Genome accession   NC_009901
Coordinates   505266..506972 (-) Length   568 a.a.
NCBI ID   WP_012153679.1    Uniprot ID   A8GZK4
Organism   Shewanella pealeana ATCC 700345     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 500266..511972
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPEA_RS02195 (Spea_0407) mutT 500954..501343 (-) 390 WP_012153673.1 8-oxo-dGTP diphosphatase MutT -
  SPEA_RS02200 (Spea_0408) yacG 501340..501579 (-) 240 WP_012153674.1 DNA gyrase inhibitor YacG -
  SPEA_RS02205 (Spea_0409) zapD 501639..502373 (-) 735 WP_012153675.1 cell division protein ZapD -
  SPEA_RS02210 (Spea_0410) coaE 502382..502987 (-) 606 WP_012153676.1 dephospho-CoA kinase -
  SPEA_RS02215 (Spea_0411) pilD 503007..503918 (-) 912 WP_012153677.1 A24 family peptidase Machinery gene
  SPEA_RS02220 (Spea_0412) pilC 503951..505219 (-) 1269 WP_012153678.1 type II secretion system F family protein Machinery gene
  SPEA_RS02225 (Spea_0413) pilB 505266..506972 (-) 1707 WP_012153679.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SPEA_RS23470 (Spea_0414) - 507007..507453 (-) 447 WP_012153680.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  SPEA_RS02235 (Spea_0415) nadC 508211..509095 (-) 885 WP_012153681.1 carboxylating nicotinate-nucleotide diphosphorylase -
  SPEA_RS02240 (Spea_0416) ampD 509338..509880 (+) 543 WP_012153682.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  SPEA_RS02245 (Spea_0417) ampE 509947..510795 (+) 849 WP_012153683.1 beta-lactamase regulator AmpE -
  SPEA_RS02250 (Spea_0418) pdhR 511158..511910 (+) 753 WP_012153684.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62800.26 Da        Isoelectric Point: 5.4755

>NTDB_id=29351 SPEA_RS02225 WP_012153679.1 505266..506972(-) (pilB) [Shewanella pealeana ATCC 700345]
MPTTGLHLGLSTLFIRKGLLNEKQLSEAVSKSRKLRQGLVATLVKDNLISAREIAELCYEEYGTPLLDLNEFEIGSIPEE
FLNKKLIEKHKCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEDDKLSKAIDSILEDDISGLDLSAIDEEA
LAGIEVTDTDKRDEEENSGKDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVNLSGRISAR
LKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGIEKLGYEDDQRLLYEEMLQKPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLSRRLCPTCKAPEAIPAEELLKLGFN
QQQVDAGIIPHKPVGCDLCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQILKQAKLQGMRDLRDSGLLKVIQGVTSIA
EINRVTSF

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=29351 SPEA_RS02225 WP_012153679.1 505266..506972(-) (pilB) [Shewanella pealeana ATCC 700345]
ATGCCAACAACCGGCCTTCACTTAGGGTTATCGACCCTCTTTATTCGCAAAGGTTTGCTTAATGAAAAGCAGCTAAGTGA
AGCCGTGTCTAAAAGTCGCAAGCTCCGTCAGGGGCTTGTGGCTACTTTAGTAAAGGATAACTTGATTAGCGCTCGTGAAA
TCGCAGAGTTATGCTACGAAGAATACGGCACACCGCTATTAGACTTAAACGAGTTCGAGATAGGCTCCATCCCCGAAGAA
TTCCTCAATAAGAAACTGATAGAGAAGCACAAATGCTTGCCACTTTTTAAGCGCGGTAATCGTCTCTATATCGCCACATC
CGACCCTACAAACATAGCAGCTTTAGAAGACTTTCAGTTTAGTGCCGGTTTGCATGCAGAAGCGATTCTAGTCGAGGACG
ATAAGCTCAGTAAAGCTATAGACTCTATTCTCGAAGACGATATTAGCGGCCTAGACTTAAGTGCGATAGACGAAGAAGCC
TTGGCAGGTATAGAAGTTACAGATACAGACAAACGTGATGAGGAAGAAAACAGTGGTAAAGACGACGCGCCGATCGTTAT
CTATATCAATAAAATCCTCACCGATGCCATTCGCAAAGGCGCATCAGATCTGCATTTTGAGCCCTATGAAAAGCGTTACC
GCATCCGTTTTCGCATCGACGGTATTTTACATGAGGTCTCCGAGCCACCAGTAAATCTTTCCGGTCGCATCTCTGCCCGC
TTAAAGGTGATGTCAAAACTTGATATTGCCGAACGCCGAGTGCCGCAAGATGGTCGTATCAAGATGAAGCTATCGCGCAC
CAAATCTATCGACTTTCGTGTCAGCACCTTGCCTACCATCTGGGGCGAGAAGATTGTAATGCGTATCTTGGACTCATCAT
CCGCTCAGCTTGGCATCGAAAAACTCGGCTATGAAGATGACCAAAGATTACTGTATGAAGAGATGCTGCAAAAACCGCAA
GGGATGATCTTGGTAACAGGCCCTACAGGCTCGGGTAAAACGGTATCGCTTTATACTGGCCTTAATATTCTTAACACCGA
AGAGCGTAATATCTCTACCGCCGAAGATCCGGTTGAGATTAATCTCGAAGGGGTCAACCAGGTTCATATCAATCTTAAGG
CAGGTCTAACCTTCGCATCAGCGCTACGTTCATTCCTGCGACAAGACCCCGATGTGGTCATGGTCGGTGAAATTCGAGAT
TTGGAAACTGCTGAAATTGCCATTAAAGCGGCGCAAACCGGGCACTTGGTTCTTTCGACTTTGCATACTAACTCCGCTGC
AGAAACACTCACCCGCTTAATCAACATGGGCGTCCCCGGTTACAACATCGCTAGCTCGGTCAACCTGATTATCGCTCAGC
GGCTATCGAGGCGCCTCTGCCCAACCTGTAAAGCGCCTGAAGCGATCCCAGCAGAAGAGTTATTAAAGCTCGGCTTTAAC
CAGCAACAAGTCGATGCAGGCATTATCCCCCACAAACCCGTGGGTTGTGACCTATGCTCCGGCGGTTATAAAGGCCGAGT
CGGTATCTATGAGGTAATGAAGATGTCGGATGAAATTGCTCGCACTATTATGGAAGGCGGTAATTCACTGCAAATCCTTA
AGCAAGCCAAACTTCAAGGCATGCGCGACTTACGAGATTCAGGCCTGCTAAAAGTCATCCAAGGCGTCACCAGTATTGCA
GAAATAAACCGCGTCACTAGCTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A8GZK4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.259

99.12

0.577
  pilB Acinetobacter baumannii D1279779

57.726

99.12

0.572
  pilB Legionella pneumophila strain ERS1305867

56.94

98.944

0.563
  pilB Vibrio parahaemolyticus RIMD 2210633

52.205

99.824

0.521
  pilB Vibrio campbellii strain DS40M4

51.418

99.296

0.511
  pilB Vibrio cholerae strain A1552

54.356

92.958

0.505
  pilF Neisseria gonorrhoeae MS11

47.719

100

0.479
  pilF Thermus thermophilus HB27

37.478

99.12

0.371
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.212

93.838

0.368

Multiple sequence alignment