Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   SPEA_RS02215 Genome accession   NC_009901
Coordinates   503007..503918 (-) Length   303 a.a.
NCBI ID   WP_012153677.1    Uniprot ID   A8GZK2
Organism   Shewanella pealeana ATCC 700345     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 498007..508918
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPEA_RS02190 (Spea_0406) - 499518..500534 (-) 1017 WP_012153491.1 IS110-like element ISSpe2 family transposase -
  SPEA_RS02195 (Spea_0407) mutT 500954..501343 (-) 390 WP_012153673.1 8-oxo-dGTP diphosphatase MutT -
  SPEA_RS02200 (Spea_0408) yacG 501340..501579 (-) 240 WP_012153674.1 DNA gyrase inhibitor YacG -
  SPEA_RS02205 (Spea_0409) zapD 501639..502373 (-) 735 WP_012153675.1 cell division protein ZapD -
  SPEA_RS02210 (Spea_0410) coaE 502382..502987 (-) 606 WP_012153676.1 dephospho-CoA kinase -
  SPEA_RS02215 (Spea_0411) pilD 503007..503918 (-) 912 WP_012153677.1 A24 family peptidase Machinery gene
  SPEA_RS02220 (Spea_0412) pilC 503951..505219 (-) 1269 WP_012153678.1 type II secretion system F family protein Machinery gene
  SPEA_RS02225 (Spea_0413) pilB 505266..506972 (-) 1707 WP_012153679.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SPEA_RS23470 (Spea_0414) - 507007..507453 (-) 447 WP_012153680.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 303 a.a.        Molecular weight: 33496.87 Da        Isoelectric Point: 7.5994

>NTDB_id=29349 SPEA_RS02215 WP_012153677.1 503007..503918(-) (pilD) [Shewanella pealeana ATCC 700345]
MTELISVFSQNPWLFIALSFVFAAVIGSFLNVVIHRMPVMMKREWQQECNHYLSEYKKQVFDSNKQQLEAPIDAFPIKYN
LVVPGSACPKCKTNIKAIHNLPMLGWLMLRGKCAACSNPISARYPIVEFITAGLVAFLAWHFGPTLEFALTALLTFCLIA
LTGIDLDEMLLPDQITLPLLWLGLIINLGGTFATTSDALIGAAAGYLSLWCVFWLFKLLTGKDGMGYGDFKLLAVFGAWF
GWQVLPLVILLSSLVGAVVGIALIVFKKLNHGNPIPFGPYIAAAGWIAMIWGESITNWYLSTL

Nucleotide


Download         Length: 912 bp        

>NTDB_id=29349 SPEA_RS02215 WP_012153677.1 503007..503918(-) (pilD) [Shewanella pealeana ATCC 700345]
ATGACTGAACTAATTTCTGTTTTTAGCCAGAATCCTTGGCTTTTTATCGCGTTAAGCTTTGTGTTTGCTGCTGTTATCGG
TAGCTTTTTAAACGTGGTGATCCACCGTATGCCGGTGATGATGAAACGTGAATGGCAGCAAGAGTGTAATCACTACCTGA
GTGAATACAAAAAGCAGGTGTTCGATAGTAATAAACAGCAACTTGAAGCGCCAATTGATGCGTTTCCTATAAAATACAAC
CTAGTCGTTCCCGGTTCTGCCTGCCCTAAATGTAAAACCAATATCAAGGCTATTCATAACCTGCCAATGCTTGGCTGGTT
GATGCTAAGAGGCAAGTGCGCAGCATGTAGTAACCCGATTTCGGCTCGCTACCCTATTGTCGAGTTTATCACTGCCGGCT
TAGTCGCTTTCTTAGCCTGGCATTTTGGCCCAACTTTAGAGTTTGCTCTAACCGCTTTACTGACTTTTTGTCTTATCGCC
TTAACAGGCATAGATCTAGACGAGATGCTATTACCCGACCAAATTACCTTGCCCCTGTTATGGCTGGGGCTGATCATTAA
CTTAGGCGGCACCTTTGCAACAACATCCGATGCCTTAATTGGCGCCGCAGCCGGTTACCTAAGTTTATGGTGCGTGTTCT
GGCTGTTTAAACTGCTTACTGGCAAGGATGGTATGGGCTATGGCGACTTTAAGTTACTAGCCGTATTTGGCGCCTGGTTC
GGCTGGCAAGTACTGCCTCTTGTTATCTTACTTTCATCGCTCGTAGGCGCAGTAGTGGGTATAGCACTTATTGTGTTCAA
GAAATTAAACCATGGCAACCCAATTCCATTTGGACCTTACATCGCTGCTGCAGGCTGGATAGCCATGATCTGGGGCGAGA
GCATTACCAACTGGTATCTGTCCACTTTATAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-142)

Type IV leader peptidase family (Peptidase_A24)

(153-261)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A8GZK2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

49.333

99.01

0.488
  pilD Vibrio campbellii strain DS40M4

49

99.01

0.485
  pilD Acinetobacter nosocomialis M2

46.844

99.34

0.465
  pilD Acinetobacter baumannii D1279779

46.512

99.34

0.462
  pilD Neisseria gonorrhoeae MS11

44.483

95.71

0.426

Multiple sequence alignment