Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   DK843_RS09770 Genome accession   NZ_CP029554
Coordinates   2004567..2006273 (-) Length   568 a.a.
NCBI ID   WP_114073139.1    Uniprot ID   A0A344UH13
Organism   Chromobacterium phragmitis strain IIBBL 274-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1999567..2011273
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DK843_RS09745 (DK843_09745) yacG 2000793..2000984 (-) 192 WP_114063600.1 DNA gyrase inhibitor YacG -
  DK843_RS09750 (DK843_09750) zapD 2000990..2001748 (-) 759 WP_168191805.1 cell division protein ZapD -
  DK843_RS09755 (DK843_09755) coaE 2001785..2002399 (-) 615 WP_114073138.1 dephospho-CoA kinase -
  DK843_RS09760 (DK843_09760) pilD 2002384..2003304 (-) 921 WP_114060249.1 prepilin peptidase Machinery gene
  DK843_RS09765 (DK843_09765) pilC 2003304..2004527 (-) 1224 WP_114060250.1 type II secretion system F family protein Machinery gene
  DK843_RS09770 (DK843_09770) pilF 2004567..2006273 (-) 1707 WP_114073139.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DK843_RS09775 (DK843_09775) - 2006442..2007566 (-) 1125 WP_114073140.1 porin -
  DK843_RS09780 (DK843_09780) - 2007963..2008283 (-) 321 WP_114073141.1 ComEA family DNA-binding protein -
  DK843_RS09785 (DK843_09785) - 2008338..2009159 (-) 822 WP_114073142.1 inner membrane protein YpjD -
  DK843_RS09790 (DK843_09790) ffh 2009271..2010626 (+) 1356 WP_114073143.1 signal recognition particle protein -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62223.83 Da        Isoelectric Point: 5.5538

>NTDB_id=293465 DK843_RS09770 WP_114073139.1 2004567..2006273(-) (pilF) [Chromobacterium phragmitis strain IIBBL 274-1]
MEATAGISGLGRALVQHNMLLQQDADAILKHAAVSHISFVEQLILSKKMTAREVAVFASRVFGYPLLDLASLDMDQLPKG
LVDEDVIASRRLLPLLKRGNKLYVGTSDPTQTSAYHAITFKTGLTVEIVVVEDDKLGRTVGRYQDSSTAALKELESEDLG
DLEFADPEAQVDSGPQVEVDDAPVVKFIHKMLLDAIGVGASDIHFEPYEKYYRIRYRVDGVLKEVAQPPLILKEKIASRI
KVISKLDISEKRLPQDGRLKLVISKTRAIDFRVSTLPTLFGEKIVMRILDSSGASLDVDQLGFEAEQKEHLLKSIARPYG
MVLVTGPTGSGKTVSLYTCLNILNKPDTNISTAEDPVEINLPGINQVNVNEKAGLTFASALKAFLRQDPDVIMVGEIRDF
ETADIAIKAAQTGHMVFSTLHTNDAPATLTRMLNMGVAPFNIASSVLLIMAQRLARKLCNHCKQPVEIPEEALLRAGFSK
DDLAEGWHPYGPVGCEECRNTGYKGRVGIFEVMPISDAMMRLIMKNGTSVDISDLAREEGMVDLRRAGLLKVMRGVTSLA
EVEAVTND

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=293465 DK843_RS09770 WP_114073139.1 2004567..2006273(-) (pilF) [Chromobacterium phragmitis strain IIBBL 274-1]
ATGGAAGCTACGGCGGGGATTTCCGGCCTGGGCAGAGCCCTGGTTCAGCACAATATGCTGCTGCAGCAGGATGCGGACGC
CATTCTGAAACATGCGGCGGTTTCCCATATCAGCTTTGTCGAGCAGCTTATCCTCAGCAAGAAAATGACGGCCAGGGAAG
TGGCGGTATTCGCTTCCCGCGTTTTCGGCTATCCGTTGCTGGACCTGGCTTCGCTCGACATGGACCAATTGCCCAAGGGG
CTGGTGGACGAGGATGTGATCGCTAGCCGCAGGCTGCTGCCCTTGCTCAAGCGCGGCAACAAGCTATATGTCGGCACTTC
GGACCCAACCCAGACTTCCGCCTACCACGCGATCACCTTCAAGACCGGGCTGACCGTAGAGATCGTCGTGGTGGAGGACG
ACAAGCTGGGGAGGACGGTCGGGCGTTATCAGGATAGTTCCACTGCGGCGCTGAAGGAGCTGGAAAGCGAAGATTTGGGC
GATCTCGAGTTTGCCGATCCCGAAGCGCAGGTGGATAGCGGGCCGCAGGTCGAAGTGGATGACGCCCCTGTGGTCAAGTT
CATCCACAAGATGTTGCTGGACGCGATAGGCGTCGGCGCGTCCGACATCCACTTCGAGCCGTATGAGAAGTATTACCGCA
TCCGCTACCGGGTGGATGGGGTGCTGAAGGAGGTGGCGCAACCGCCGTTGATCCTGAAGGAAAAGATCGCTTCGCGCATC
AAGGTGATTTCCAAGCTGGATATCTCGGAAAAGCGGTTGCCGCAGGATGGGCGGCTGAAGCTGGTGATTTCCAAGACCCG
GGCGATAGATTTTCGCGTCAGCACGCTGCCCACGCTGTTCGGCGAGAAGATCGTGATGCGTATTCTGGACTCCTCGGGCG
CGTCGCTGGATGTCGACCAGCTTGGCTTCGAGGCGGAGCAGAAAGAGCATTTGCTGAAGTCGATCGCCCGCCCTTACGGC
ATGGTGCTGGTCACCGGCCCCACCGGCAGCGGCAAGACGGTGTCGCTGTACACTTGTCTCAACATCCTTAATAAACCGGA
TACCAATATCTCCACCGCGGAGGACCCGGTGGAAATCAATTTGCCCGGCATCAATCAGGTCAACGTCAATGAAAAGGCTG
GACTGACTTTCGCATCGGCGCTGAAGGCTTTCCTTAGACAGGACCCGGACGTGATCATGGTGGGGGAAATCCGCGACTTT
GAAACCGCCGACATCGCGATCAAGGCGGCGCAGACGGGGCACATGGTGTTTTCCACGCTGCATACCAACGACGCGCCCGC
CACGCTGACCCGGATGTTGAACATGGGCGTGGCGCCGTTCAATATCGCCAGTTCGGTTTTGCTGATCATGGCGCAGCGCC
TGGCGCGCAAGCTGTGCAATCATTGCAAGCAGCCGGTGGAGATTCCGGAGGAGGCCTTGCTGCGCGCCGGGTTTTCCAAG
GACGACCTGGCGGAGGGATGGCATCCCTATGGTCCTGTCGGCTGTGAGGAGTGCCGCAATACCGGGTACAAGGGACGGGT
CGGCATATTCGAAGTGATGCCCATCAGCGATGCGATGATGCGGCTGATCATGAAGAACGGGACCTCGGTGGATATCTCCG
ACTTGGCGCGGGAGGAAGGCATGGTCGACTTGCGCCGCGCCGGCTTGCTGAAAGTGATGCGCGGCGTGACCTCGCTGGCC
GAGGTCGAGGCGGTGACCAACGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-148)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A344UH13

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

57.522

99.472

0.572
  pilB Acinetobacter baumannii D1279779

53.78

100

0.551
  pilB Acinetobacter baylyi ADP1

54.174

99.12

0.537
  pilB Legionella pneumophila strain ERS1305867

53.345

100

0.533
  pilB Vibrio cholerae strain A1552

49.372

98.063

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

48.49

99.12

0.481
  pilB Vibrio campbellii strain DS40M4

47.247

99.12

0.468
  pilF Thermus thermophilus HB27

38.078

98.944

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.634

98.239

0.37

Multiple sequence alignment