Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   DJ564_RS03030 Genome accession   NZ_CP029482
Coordinates   645934..648009 (+) Length   691 a.a.
NCBI ID   WP_109627575.1    Uniprot ID   A0A2U8TK94
Organism   Pseudomonas sp. 31-12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 640934..653009
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DJ564_RS03010 (DJ564_03010) comM 643146..644210 (+) 1065 WP_109627569.1 pilus assembly protein PilM Machinery gene
  DJ564_RS03015 (DJ564_03015) - 644210..644776 (+) 567 WP_109627570.1 PilN domain-containing protein -
  DJ564_RS03020 (DJ564_03020) pilO 644773..645396 (+) 624 WP_109627572.1 type 4a pilus biogenesis protein PilO -
  DJ564_RS03025 (DJ564_03025) - 645393..645920 (+) 528 WP_109627573.1 pilus assembly protein PilP -
  DJ564_RS03030 (DJ564_03030) pilQ 645934..648009 (+) 2076 WP_109627575.1 type IV pilus secretin PilQ Machinery gene
  DJ564_RS03035 (DJ564_03035) aroK 648014..648532 (+) 519 WP_010464421.1 shikimate kinase AroK -
  DJ564_RS03040 (DJ564_03040) aroB 648684..649784 (+) 1101 WP_109627577.1 3-dehydroquinate synthase -
  DJ564_RS03045 (DJ564_03045) - 649795..651393 (+) 1599 WP_109627578.1 AAA family ATPase -

Sequence

Protein


Download         Length: 691 a.a.        Molecular weight: 74871.10 Da        Isoelectric Point: 5.7826

>NTDB_id=293078 DJ564_RS03030 WP_109627575.1 645934..648009(+) (pilQ) [Pseudomonas sp. 31-12]
MNRIFSTLGISLWIALLSPMVQAANLKALDVAALPGDRVELKLSFDSPPPQPHGYTTESPARIALDLPGVVSQLKSKNRE
LGGGNARSATVVEAKDRTRLIINLTQLTPYSARVEGNNLIVVVGQGATNKASRPPASAPRAATATPAPTKAYAPVGKAIR
GVDFQRGTQGEGNVVIDLSDPSIAPDIQERDGKIILGFARTQLPEPLRVRLDVKDFATPVQFVNASATGDRATISIEPSG
AFDYSTYQTDNKLTVSIRPMTVDDLQKRNAERFAYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQISELAPLRRELLQVNYAKAADIAKLFTSVTSAEA
KIDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQKGNWNTSGVNG
SSTTIGTPGSTSTNSPFVDLGTSANTSGIGIAFITDNVLLDLELSAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQ
EASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNT
QSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2076 bp        

>NTDB_id=293078 DJ564_RS03030 WP_109627575.1 645934..648009(+) (pilQ) [Pseudomonas sp. 31-12]
ATGAACAGGATTTTCTCAACCCTCGGTATCTCGCTATGGATAGCGTTGCTGTCGCCGATGGTACAAGCGGCCAACCTCAA
AGCGCTGGATGTCGCCGCGCTACCGGGTGATCGTGTCGAGTTGAAGTTGTCTTTCGATAGCCCGCCCCCGCAACCCCACG
GTTACACGACCGAGTCGCCCGCACGCATTGCGCTGGATCTGCCCGGCGTTGTCAGTCAGCTCAAAAGCAAAAATCGTGAA
CTGGGCGGCGGAAATGCCCGTAGCGCCACAGTGGTGGAAGCCAAGGACCGTACGCGACTTATCATCAATCTGACTCAGTT
AACCCCTTACAGCGCACGGGTTGAGGGCAACAATCTGATCGTGGTGGTCGGGCAAGGCGCCACCAACAAAGCGTCCCGCC
CGCCCGCCAGCGCACCCCGCGCCGCGACTGCAACGCCAGCCCCGACGAAAGCCTACGCACCCGTGGGCAAGGCCATCCGT
GGTGTGGATTTCCAGCGCGGCACGCAGGGTGAAGGCAATGTGGTGATTGATCTGTCGGATCCTTCCATCGCCCCGGATAT
TCAGGAACGCGACGGCAAGATCATCCTCGGTTTTGCCAGAACCCAATTGCCAGAGCCATTGCGTGTGCGCCTGGACGTCA
AGGATTTCGCCACCCCGGTGCAGTTCGTCAATGCCAGCGCCACGGGCGACCGGGCCACAATCAGCATTGAGCCCAGCGGC
GCGTTCGATTATTCGACCTACCAGACTGACAACAAGCTGACGGTCAGCATCCGGCCGATGACGGTCGATGACCTGCAAAA
GCGCAACGCCGAGCGTTTTGCCTATACCGGTGAAAAGCTCTCGCTGAATTTCCAGGACATCGATGTGCGTTCGGTGCTGC
AACTGATCGCCGATTTCACCAACCTCAATCTGGTGGCCAGCGACACGGTGCAGGGCGGCATCACCTTGCGTTTGCAGAAC
GTGCCGTGGGACCAGGCGCTGGACCTGGTGCTAAAAACCAAAGGCCTGGACAAGCGCAAGATCGGCAACGTCCTGCTGGT
GGCGCCGGCCGATGAAATCGCGGCGCGCGAGCGTCAGGAACTGGAGTCGCAGAAGCAAATCTCCGAGCTTGCGCCCCTGC
GTCGTGAGTTGTTGCAGGTCAACTACGCGAAGGCTGCCGATATCGCCAAGCTGTTCACCTCGGTGACCAGTGCCGAAGCG
AAAATCGACGAGCGGGGTTCTATCACGGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCAGGACCGACTCGA
CGAACTTCGTCGTATCGTCGCGCAACTGGATATCCCGGTGCGCCAGGTCATGATCGAGGCGCGAATCGTCGAAGCCAACG
TCGACTACGACAAGAGCCTCGGCGTGCGCTGGGGTGGTTCGGTGCAGAAGGGCAACTGGAACACGTCTGGGGTCAACGGC
TCTTCGACCACCATTGGTACGCCGGGCAGCACCAGTACCAATTCGCCGTTCGTCGACTTGGGGACCTCTGCCAATACTTC
GGGGATCGGCATCGCCTTCATTACCGACAATGTGCTGCTGGACCTTGAGCTTTCGGCCATGGAGAAAACCGGCAACGGGG
AAATCGTTTCGCAGCCCAAGGTGGTCACGTCCGACAAGGAGACCGCGAAAATCCTCAAGGGCACCGAGATTCCCTATCAG
GAGGCCAGCTCCAGCGGTGCCACGTCGGTGTCGTTCAAGGAGGCGTCGCTGTCCCTTGAGGTCACGCCACAGATCACCCC
GGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGACTATCTGAACAAGGTCCAGGATGTGCCGCCGA
TCAAGAAAAACGAGGTCAATGCCAAGGTCCTGGTCAATGATGGCGAGACCATCGTCATTGGCGGGGTTTTCTCAAATACT
CAGAGCAAGGTTGTAGATAAGGTGCCATTTCTCGGTGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGTTTC
GGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCATTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

Secretin and TonB N terminus short domain (STN)

(303-349)

AMIN domain (AMIN)

(38-122)

Bacterial type II and III secretion system protein (Secretin)

(526-682)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2U8TK94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.669

100

0.761
  pilQ Acinetobacter baumannii strain A118

38.911

100

0.424
  pilQ Acinetobacter baumannii D1279779

38.911

100

0.424
  comQ Acinetobacter baylyi ADP1

42.197

100

0.423
  pilQ Legionella pneumophila strain Lp02

38.088

98.408

0.375
  pilQ Legionella pneumophila strain ERS1305867

37.962

97.974

0.372

Multiple sequence alignment