Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   DJ564_RS03010 Genome accession   NZ_CP029482
Coordinates   643146..644210 (+) Length   354 a.a.
NCBI ID   WP_109627569.1    Uniprot ID   A0A2U8TLI1
Organism   Pseudomonas sp. 31-12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 638146..649210
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DJ564_RS03000 (DJ564_03000) - 638947..640215 (+) 1269 WP_010464435.1 malic enzyme-like NAD(P)-binding protein -
  DJ564_RS03005 (DJ564_03005) - 640484..642928 (-) 2445 WP_371922070.1 penicillin-binding protein 1A -
  DJ564_RS03010 (DJ564_03010) comM 643146..644210 (+) 1065 WP_109627569.1 pilus assembly protein PilM Machinery gene
  DJ564_RS03015 (DJ564_03015) - 644210..644776 (+) 567 WP_109627570.1 PilN domain-containing protein -
  DJ564_RS03020 (DJ564_03020) pilO 644773..645396 (+) 624 WP_109627572.1 type 4a pilus biogenesis protein PilO -
  DJ564_RS03025 (DJ564_03025) - 645393..645920 (+) 528 WP_109627573.1 pilus assembly protein PilP -
  DJ564_RS03030 (DJ564_03030) pilQ 645934..648009 (+) 2076 WP_109627575.1 type IV pilus secretin PilQ Machinery gene
  DJ564_RS03035 (DJ564_03035) aroK 648014..648532 (+) 519 WP_010464421.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38057.51 Da        Isoelectric Point: 4.6091

>NTDB_id=293077 DJ564_RS03010 WP_109627569.1 643146..644210(+) (comM) [Pseudomonas sp. 31-12]
MLGLFNKKANTLLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPANAVVEKNIAELEGVGQALSRVLLKAKTNLRNVAV
AVAGSAVITKTIEMDAGLSDDEMENQLKIEADQYIPYPLDEVAIDFEVQGASARNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFSLLAGQLAVSQEGLTVAVVDIGATMTTLSVLYNGRIIYTREQLFGGRQLTEEIQRRYGLTI
EQAGLAKKQGGLPDDYVSEVLQPFRDALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLGTPTQVANP
FSDMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=293077 DJ564_RS03010 WP_109627569.1 643146..644210(+) (comM) [Pseudomonas sp. 31-12]
GTGCTAGGACTCTTCAATAAAAAAGCCAACACGCTTCTGGGGATCGACATCAGCTCCACGTCGGTGAAGCTGCTTGAGCT
AAGTCGCCAGGGCGAGCGTTATCGGGTCGAGGCCTATGCGGTCGAACCCTTGCCCGCCAACGCAGTGGTCGAAAAAAATA
TCGCCGAGCTCGAAGGCGTGGGTCAGGCTCTCTCTCGCGTGTTGCTCAAGGCCAAAACCAATCTCAGAAATGTCGCCGTA
GCTGTCGCCGGATCGGCAGTCATCACCAAAACCATTGAGATGGATGCAGGTCTTTCCGACGACGAGATGGAAAACCAGCT
GAAGATCGAGGCCGATCAGTACATCCCCTATCCGTTGGATGAAGTCGCCATCGACTTCGAAGTCCAGGGCGCGTCGGCGC
GTAACCCCGAGCGGGTCAATGTGCTGCTGGCGGCCTGTCGCAAGGAAAACGTCGAAGTGCGTGAAGCCGCCCTGGCGCTG
GCCGGCCTGACGGCGCGCGTGGTGGACGTCGAGGCTTATGCGCTGGAGCGATCGTTCTCGTTACTGGCCGGGCAACTGGC
GGTTTCGCAGGAGGGGCTGACCGTGGCGGTGGTCGACATCGGCGCGACCATGACCACCCTGAGCGTGCTGTACAACGGGC
GGATCATCTACACCCGCGAACAATTGTTCGGCGGTCGGCAGCTGACGGAGGAAATCCAGCGTCGTTATGGCCTGACCATC
GAGCAGGCGGGGCTGGCGAAGAAGCAGGGCGGGTTGCCGGACGATTACGTCAGCGAAGTCTTGCAGCCGTTTCGCGATGC
GCTGGTGCAGCAGGTTTCCCGGTCGTTGCAGTTCTTCTTCGCCTCCGGTCAGTACAACGCGGTCGATCACATCCTGCTGG
CGGGCGGTACCGCGTCAGTCCCGGGGCTTGATCGCTTGATCGAACAGCGGCTCGGCACACCGACCCAGGTGGCCAACCCG
TTCTCCGACATGGCGTTGAGCAGCAAGGTCAACGCCGGCGCCCTGGCAAGCGACGCGCCAGCACTGATGATCGCCTGCGG
GCTTGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2U8TLI1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

57.627

100

0.576
  pilM Acinetobacter baumannii D1279779

57.345

100

0.573
  comM Acinetobacter baylyi ADP1

55.085

100

0.551
  pilM Legionella pneumophila strain ERS1305867

48.87

100

0.489

Multiple sequence alignment