Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DJ533_RS17385 Genome accession   NZ_CP029397
Coordinates   3109701..3111416 (+) Length   571 a.a.
NCBI ID   WP_065993783.1    Uniprot ID   A0A2S2FIM7
Organism   Acinetobacter defluvii strain WCHA30     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3104701..3116416
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DJ533_RS17345 (DJ533_17385) nusA 3105253..3106737 (-) 1485 WP_065993779.1 transcription termination factor NusA -
  DJ533_RS17350 (DJ533_17390) rimP 3106778..3107302 (-) 525 WP_065993780.1 ribosome maturation factor RimP -
  DJ533_RS17375 (DJ533_17415) secG 3108112..3108444 (-) 333 WP_065993781.1 preprotein translocase subunit SecG -
  DJ533_RS17380 (DJ533_17420) tpiA 3108454..3109248 (-) 795 WP_065993782.1 triose-phosphate isomerase -
  DJ533_RS17385 (DJ533_17425) pilB 3109701..3111416 (+) 1716 WP_065993783.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DJ533_RS17390 (DJ533_17430) pilC 3111443..3112669 (+) 1227 WP_065993784.1 type II secretion system F family protein Machinery gene
  DJ533_RS17395 (DJ533_17435) pilD 3112669..3113529 (+) 861 WP_065993785.1 A24 family peptidase Machinery gene
  DJ533_RS17400 (DJ533_17440) coaE 3113542..3114138 (+) 597 WP_065993786.1 dephospho-CoA kinase -
  DJ533_RS17405 (DJ533_17445) - 3114202..3115122 (-) 921 WP_065993787.1 DMT family transporter -
  DJ533_RS17410 (DJ533_17450) rlmB 3115163..3115915 (-) 753 WP_065993788.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  DJ533_RS17415 (DJ533_17455) - 3116055..3116378 (-) 324 WP_065993789.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63537.05 Da        Isoelectric Point: 4.9519

>NTDB_id=292242 DJ533_RS17385 WP_065993783.1 3109701..3111416(+) (pilB) [Acinetobacter defluvii strain WCHA30]
MVLQQGSPKFSGFIRRLVDDGVLSAQQMQSALDNAKKNKLNIVAYLIEYANLKPLQLAETIALEFAEPLFDLDSFDSVQF
PKEVIESNLLHKHLMLPLVQHGNVLYIAISDPTNIEALDAARFNSRLNIEVVIVEHHKLLKCLEKYFTEETNFDFGGDDI
DVDIISTDESNTPSDQDDQPSEDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRSITNPPLQLATRLA
SRLKVMSQMDISEKRIPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALEK
PQGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSVALKSFLRQDPDIIMVGEI
RDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPHCKAPIEIPKNSLLEMG
FTEEDIAHPDFQIFQPVGCPECREGYKGRVGVYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLIKVMNGLT
SLQEINRVTSE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=292242 DJ533_RS17385 WP_065993783.1 3109701..3111416(+) (pilB) [Acinetobacter defluvii strain WCHA30]
ATGGTGTTACAACAAGGTTCGCCGAAGTTTTCGGGATTTATTCGTCGCTTAGTCGATGACGGTGTTCTTTCTGCGCAGCA
AATGCAGTCTGCCTTAGACAATGCCAAAAAAAACAAGCTTAATATTGTGGCTTATTTAATCGAATATGCAAATTTAAAAC
CTTTACAGTTGGCTGAGACCATTGCTTTAGAGTTTGCAGAACCGTTATTTGACTTAGATAGCTTTGATAGTGTGCAATTT
CCCAAAGAGGTCATTGAAAGTAATCTCTTACATAAACACTTAATGTTACCCTTGGTTCAACATGGCAATGTTTTATATAT
CGCAATCAGCGACCCAACCAATATTGAAGCGCTTGATGCAGCACGTTTTAATAGCCGCTTAAATATCGAAGTGGTGATTG
TAGAGCATCATAAACTTCTAAAATGTTTAGAAAAATATTTTACAGAAGAAACCAATTTTGATTTTGGTGGAGATGATATT
GATGTTGATATTATTTCAACAGATGAATCCAACACGCCTAGCGATCAAGATGATCAACCCTCTGAAGATGAAGCGCCTAT
TGTCAAATATATCAATAAGTTACTTATTGATGCAATTCGTATGGGTGCCTCTGATTTACATTTTGAACCTTATGAAAAAT
CTTATCGAGTTCGTTACCGTGTAGATGGTGTACTCAGAAGCATTACCAATCCTCCACTACAATTGGCGACACGTTTGGCA
TCACGTTTAAAAGTCATGTCACAAATGGACATATCAGAGAAGCGTATTCCTCAAGATGGTCGTATTAAGCTCAAGCTCTC
TAAAACCAAAGCCATTGACTTTCGTGTTAACTCACTTCCTACTTTATTTGGCGAAAAGCTTGTACTGCGTATTTTAGATC
CATCCAGTGCGATGCTAGGGATTGATGCGCTGGGTTATGAGGAAGAGCAAAAAGCTTTGTTTATGGAAGCCTTAGAAAAA
CCTCAAGGCATGTTACTCATTACAGGTCCTACAGGTTCAGGTAAAACGGTTTCCTTATATACGGGTTTAAACATTCTGAA
TACAGAAAGTTCAAATATTTCGACAGCCGAAGACCCTGTGGAAATTAACTTAGAAGGGATTAACCAAGTCAACGTCAATC
CTAAAGTTGGTCTTACCTTCTCGGTAGCTTTAAAATCTTTTTTACGTCAGGATCCAGACATTATCATGGTCGGGGAGATT
CGAGACTTAGAAACTGCAGAAATTGCGATTAAAGCCGCACAAACTGGGCACATGGTGATGTCTACACTACACACCAATAG
TGCGCCAGAAACATTAACACGTTTAAGAAATATGGGAGTTCCCTCCTTTAATATCGCAACATCAGTCAATCTTGTGATCG
CACAGCGTTTAGCACGTCGTCTATGTCCACATTGTAAAGCACCCATTGAAATTCCTAAAAATAGCTTATTGGAAATGGGC
TTTACTGAGGAAGACATTGCCCATCCAGATTTTCAAATTTTTCAACCTGTCGGATGTCCAGAATGCCGTGAAGGCTATAA
AGGACGAGTTGGGGTTTATGAAGTCATGAAAGTAACACCTGAAATTTCAAAAATTATTATGGAGGATGGTAATGCGCTTG
AAATTGCAGCAGCATCTGAAAAACTGGGGTTTAATAATTTGCGACGTTCAGGTCTTATCAAAGTCATGAATGGTCTAACA
TCTCTACAAGAAATTAATCGCGTTACGAGCGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S2FIM7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.708

98.949

0.799
  pilB Acinetobacter baylyi ADP1

76.95

98.774

0.76
  pilF Neisseria gonorrhoeae MS11

49.734

98.599

0.49
  pilB Legionella pneumophila strain ERS1305867

50.926

94.571

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

48.115

97.548

0.469
  pilB Vibrio campbellii strain DS40M4

47.74

96.848

0.462
  pilB Vibrio cholerae strain A1552

47.387

97.198

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

34.967

100

0.375

Multiple sequence alignment