Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DGN21_RS15045 Genome accession   NZ_CP029263
Coordinates   3580569..3582305 (-) Length   578 a.a.
NCBI ID   WP_126966294.1    Uniprot ID   -
Organism   Xanthomonas sp. MLO165     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3575569..3587305
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DGN21_RS15030 (DGN21_15390) sucC 3575747..3576916 (-) 1170 WP_039519379.1 ADP-forming succinate--CoA ligase subunit beta -
  DGN21_RS15035 (DGN21_15395) - 3577148..3578761 (+) 1614 WP_016901263.1 HAMP domain-containing sensor histidine kinase -
  DGN21_RS15040 (DGN21_15400) pilR 3578954..3580348 (+) 1395 WP_016901261.1 sigma-54 dependent transcriptional regulator Regulator
  DGN21_RS15045 (DGN21_15405) pilB 3580569..3582305 (-) 1737 WP_126966294.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DGN21_RS20320 (DGN21_15415) - 3582937..3583389 (-) 453 WP_104600101.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  DGN21_RS15060 (DGN21_15420) pilC 3583743..3584996 (+) 1254 WP_104608920.1 type II secretion system F family protein Machinery gene
  DGN21_RS15065 (DGN21_15425) - 3585004..3585867 (+) 864 WP_016849610.1 A24 family peptidase -
  DGN21_RS15070 (DGN21_15430) coaE 3585881..3586504 (+) 624 WP_126966295.1 dephospho-CoA kinase -
  DGN21_RS15075 (DGN21_15435) - 3586604..3586819 (+) 216 Protein_2971 SymE family type I addiction module toxin -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62679.88 Da        Isoelectric Point: 5.5986

>NTDB_id=291467 DGN21_RS15045 WP_126966294.1 3580569..3582305(-) (pilB) [Xanthomonas sp. MLO165]
MSIAISTNLVGITGLARRLVQDGALDEMAARAAMDQAAQAKVPLPQWFADRKLVSAAQLAAANAVEFGMPLMDVSVFDAS
QNAIKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSLG
DDDEGMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPAQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQSIGIRDLRQSALV
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=291467 DGN21_RS15045 WP_126966294.1 3580569..3582305(-) (pilB) [Xanthomonas sp. MLO165]
ATGAGCATCGCAATCAGCACCAACTTGGTCGGTATCACTGGGCTGGCGCGTCGTCTTGTGCAAGATGGTGCTCTTGATGA
AATGGCGGCCCGTGCTGCAATGGATCAGGCTGCGCAAGCGAAGGTGCCCCTCCCGCAATGGTTCGCGGACAGGAAGCTGG
TGTCCGCGGCACAACTTGCGGCGGCTAATGCAGTCGAGTTCGGCATGCCTCTAATGGACGTTTCCGTCTTCGACGCCAGC
CAAAACGCCATTAAGCTAGTTAGTGAAGAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
ATTCGTGGGGGTGAGCAACCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCATACCAACCTGGTGGTCGAGCCGA
TCCTGGTCGACGAGGACCAGATCCGTCGCACCTTGGAGCAATGGCAAGCCAGCAATGCGGCGCTTGGCTCGTCGCTCGGG
GACGATGATGAGGGGATGGGCGACCTGGACGTGTCGGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACA
TCCACTTCGAGCCGTACGAAGACGACTATCGGGTGCGCTTGCGCATCGATGGGCTGCTGAAGAATGTTGCCAAGGCACCG
GTAAAGCTGAACCAGCGCATCGCGGCGCGTCTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCTCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACTAAGCAGATCGACTTCCGCGTGAGCACCTTGCCGACGTTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGAGAAGCTGGGCTACGAGGCGGACCAGCAGAAG
CTGTTCCTGGAGGCGATCCACAAACCCTACGGGATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTATACGGCACTCGGCATCCTCAACGACGAGACGCGCAACATCTCCACCGCCGAGGACCCCGTTGAAATTCGCTTGCCTG
GCGTCAATCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTTGCCGCGGCGCTGCGTTCGTTCTTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCCGCGCAGACCGGTCACATGGT
GCTGTCCACGCTGCACACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCA
CCTCGTCGGTGACCCTGGTGATCGCGCAGCGTCTGGCGCGGCGCCTGTGCAACAACTGCAAGCGCAAATCGACCTTGCCC
GAGCACGCGTTGCTGGCCGAAGGCTTCACGCCGGCGCAGATCGCTGCCGGTATCGAGTTGTATGAGGCGGTCGGCTGCGA
CGAATGCACCGAAGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAAGGCGGTAACGCGATGCAGATCGCCGAGGCGGCACAGAGTATCGGCATCCGCGACCTGCGCCAATCGGCGTTGGTC
AAGGCCGCGCACGGGGTAACCAGCCTGGCCGAGATCAACCGAGTGACGAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.81

98.27

0.548
  pilB Acinetobacter baylyi ADP1

55.634

98.27

0.547
  pilB Legionella pneumophila strain ERS1305867

51.557

100

0.516
  pilB Vibrio cholerae strain A1552

48.789

100

0.488
  pilF Neisseria gonorrhoeae MS11

48.592

98.27

0.478
  pilB Vibrio campbellii strain DS40M4

45.694

98.443

0.45
  pilB Vibrio parahaemolyticus RIMD 2210633

45.76

97.924

0.448
  pilF Thermus thermophilus HB27

40.192

89.965

0.362

Multiple sequence alignment