Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   CSC28_RS29800 Genome accession   NZ_CP029093
Coordinates   5960467..5962611 (-) Length   714 a.a.
NCBI ID   WP_003148771.1    Uniprot ID   A0A2R3IXQ2
Organism   Pseudomonas paraeruginosa strain AR441     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5955467..5967611
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSC28_RS29785 (CSC28_5908) - 5957128..5958774 (-) 1647 WP_053814715.1 AAA family ATPase -
  CSC28_RS29790 (CSC28_5909) aroB 5958781..5959887 (-) 1107 WP_012077719.1 3-dehydroquinate synthase -
  CSC28_RS29795 (CSC28_5910) aroK 5959937..5960455 (-) 519 WP_003148769.1 shikimate kinase AroK -
  CSC28_RS29800 (CSC28_5911) pilQ 5960467..5962611 (-) 2145 WP_003148771.1 type 4a pilus secretin PilQ Machinery gene
  CSC28_RS29805 (CSC28_5912) pilP 5962665..5963189 (-) 525 WP_034082521.1 type 4a pilus biogenesis lipoprotein PilP -
  CSC28_RS29810 (CSC28_5913) pilO 5963186..5963809 (-) 624 WP_003148775.1 type 4a pilus biogenesis protein PilO -
  CSC28_RS29815 (CSC28_5914) pilN 5963806..5964402 (-) 597 WP_003148777.1 type 4a pilus biogenesis protein PilN -
  CSC28_RS29820 (CSC28_5915) pilM 5964402..5965466 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 714 a.a.        Molecular weight: 77412.06 Da        Isoelectric Point: 5.3173

>NTDB_id=290454 CSC28_RS29800 WP_003148771.1 5960467..5962611(-) (pilQ) [Pseudomonas paraeruginosa strain AR441]
MNSGLSRLGIALLAAMFAPALLAADLEKLDVAALPGDRVELKLQFDQPVATPRGYTIEQPARIALDLPGVQNKLGTKNRE
LSVGNTRSVTVVEAKDRTRLIINLTTLSSYTTRVEGNNLFVVVGNSPAGASVASAAPVKASPAPASYAQPIKPKPYVPAG
RAIRNIDFQRGEKGEGNVVIDLSDPTLSPDIQEQGGKIRLDFAKTQLPDALRVRLDVKDFATPVQFVNASAQSDRTSITI
EPSGLYDYLVYQTDNRLTVSIKPMTADDAERRKKDNFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITL
RLQNVPWDQALDLVLKTKGLDKRKLGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKAADIAKLFQSVT
SEGGAEGKEGGRGSITVDDRTNSIIAYQPQDRLDELRRIVSQLDIPVRQVMIEARIVEANVGYDKSLGVRWGGAYHKGNW
NAYGKDGNMGIKDEEGLNCGPIAGNCSFPTVGTNKSPSPFVDIGAKDATSGIGIGFITDNIILDLQLSAMEKTGNGEIIS
QPKVVTSDKETAKILKGSEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIVEVKVTKDAPDYQNMLNGVPPINKN
EVNAKILVNDGETIVIGGVFSNEQTKAVDKVPFLGDLPYLGRLFRRDTVVDRKNELLVFLTPRIMNNQAIAIGR

Nucleotide


Download         Length: 2145 bp        

>NTDB_id=290454 CSC28_RS29800 WP_003148771.1 5960467..5962611(-) (pilQ) [Pseudomonas paraeruginosa strain AR441]
ATGAACAGTGGCCTCTCGCGCCTCGGGATCGCTTTGCTGGCCGCCATGTTCGCGCCGGCCTTGCTCGCCGCGGACCTGGA
GAAACTCGACGTGGCGGCGCTGCCCGGAGACCGGGTCGAGCTCAAGCTGCAGTTCGACCAGCCCGTCGCCACGCCGCGCG
GCTATACCATCGAGCAGCCGGCGCGGATCGCCCTCGACCTGCCGGGGGTGCAGAACAAGCTCGGCACCAAGAACCGCGAA
CTGAGCGTGGGCAATACACGCAGCGTCACGGTGGTCGAGGCGAAGGACCGCACACGGCTGATCATCAACCTGACCACGCT
GTCGTCCTACACCACCCGGGTGGAGGGCAACAACCTGTTCGTGGTGGTCGGCAATTCGCCGGCCGGCGCCAGCGTCGCTT
CCGCCGCTCCGGTCAAGGCGAGCCCGGCTCCGGCCAGCTACGCCCAGCCGATCAAGCCCAAGCCGTACGTGCCGGCGGGC
CGCGCGATCCGCAATATCGACTTCCAGCGCGGCGAGAAGGGCGAGGGCAACGTGGTCATCGACCTCTCCGACCCGACGCT
GAGCCCGGATATCCAGGAGCAGGGCGGCAAGATCCGCCTGGACTTCGCCAAGACCCAGCTGCCCGACGCGCTGCGGGTTC
GCCTGGACGTCAAGGACTTCGCCACCCCGGTGCAGTTCGTCAACGCCAGTGCGCAATCCGACCGTACCAGCATCACCATC
GAGCCGAGCGGGCTCTACGACTACCTGGTCTACCAGACCGACAATCGCCTGACCGTCAGCATCAAGCCGATGACCGCGGA
CGACGCCGAGCGGCGCAAGAAGGACAACTTCGCCTACACCGGCGAGAAGCTGTCGCTGAACTTCCAGGACATCGACGTGC
GGTCGGTGCTGCAATTGATCGCCGACTTCACCGATCTGAACCTGGTGGCCTCGGATACCGTGCAGGGCAACATCACCCTG
CGCCTGCAGAATGTGCCGTGGGACCAGGCGCTGGACCTCGTGCTCAAGACCAAGGGCCTGGACAAGCGCAAGCTCGGCAA
CGTGCTGCTGGTGGCGCCGGCCGACGAGATCGCTGCACGCGAGCGTCAGGAGCTGGAAGCGCAGAAGCAGATCGCCGAAC
TGGCGCCGCTGCGTCGCGAGCTGATCCAGGTGAACTACGCCAAGGCGGCGGACATCGCCAAGCTGTTCCAGTCGGTGACC
AGCGAGGGCGGTGCGGAAGGCAAGGAAGGCGGGCGCGGCTCGATCACCGTGGACGACCGCACCAACAGCATCATCGCCTA
CCAGCCGCAAGATCGCCTGGATGAGCTGCGGCGGATCGTTTCGCAACTGGACATCCCGGTACGCCAGGTGATGATCGAGG
CGCGCATCGTCGAAGCCAACGTCGGCTACGACAAGAGCCTCGGCGTGCGCTGGGGCGGTGCCTACCACAAGGGCAACTGG
AATGCCTACGGCAAGGACGGCAACATGGGGATCAAGGACGAGGAAGGCCTGAACTGCGGTCCCATCGCCGGCAACTGTAG
CTTCCCCACCGTGGGCACCAACAAGTCGCCGTCGCCGTTCGTCGATATTGGCGCGAAGGATGCCACTTCCGGGATCGGTA
TCGGCTTCATCACCGACAACATCATCCTCGACCTGCAATTGTCGGCGATGGAAAAGACCGGCAACGGTGAAATCATTTCC
CAGCCGAAGGTGGTCACTTCCGACAAGGAAACCGCGAAGATCCTCAAAGGCTCCGAGGTTCCCTATCAGGAGGCCAGTTC
CAGCGGCGCCACCAGTACCTCCTTCAAGGAGGCTGCGCTGTCCCTCGAAGTGACCCCGCAGATCACGCCGGACAACCGGA
TCATCGTCGAGGTCAAGGTCACCAAGGATGCGCCGGACTACCAGAACATGCTGAATGGCGTACCGCCGATCAACAAGAAC
GAGGTCAACGCCAAGATCCTGGTCAACGATGGCGAAACCATCGTCATCGGCGGGGTGTTCAGCAACGAGCAGACCAAGGC
GGTGGACAAGGTGCCGTTCCTCGGCGACCTGCCCTACCTGGGCCGCCTGTTCCGGCGTGACACGGTGGTCGACAGGAAAA
ACGAACTTCTGGTTTTCCTGACACCGCGGATCATGAATAATCAGGCCATCGCAATCGGTCGCTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-122)

Bacterial type II and III secretion system protein (Secretin)

(549-705)

Bacterial type II/III secretion system short domain (Secretin_N)

(381-449)

Secretin and TonB N terminus short domain (STN)

(307-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2R3IXQ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

96.639

100

0.966
  comQ Acinetobacter baylyi ADP1

40.782

100

0.409
  pilQ Acinetobacter baumannii D1279779

40.278

100

0.406
  pilQ Acinetobacter baumannii strain A118

40.278

100

0.406
  pilQ Legionella pneumophila strain Lp02

37.662

97.059

0.366
  pilQ Legionella pneumophila strain ERS1305867

37.662

97.059

0.366

Multiple sequence alignment