Detailed information NTG0290455

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: CSC28_RS29820
• Genome accession: NZ_CP029093
• Coordinates: 5964402..5965466 (-)
• Length: 354 a.a.
• NCBI ID: WP_003110888.1
• Uniprot ID: -
• Organism: Pseudomonas paraeruginosa strain AR441
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5959402..5970466

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CSC28_RS29795 (CSC28_5910)	aroK	5959937..5960455 (-)	519	WP_003148769.1	shikimate kinase AroK	-
CSC28_RS29800 (CSC28_5911)	pilQ	5960467..5962611 (-)	2145	WP_003148771.1	type 4a pilus secretin PilQ	Machinery gene
CSC28_RS29805 (CSC28_5912)	pilP	5962665..5963189 (-)	525	WP_034082521.1	type 4a pilus biogenesis lipoprotein PilP	-
CSC28_RS29810 (CSC28_5913)	pilO	5963186..5963809 (-)	624	WP_003148775.1	type 4a pilus biogenesis protein PilO	-
CSC28_RS29815 (CSC28_5914)	pilN	5963806..5964402 (-)	597	WP_003148777.1	type 4a pilus biogenesis protein PilN	-
CSC28_RS29820 (CSC28_5915)	pilM	5964402..5965466 (-)	1065	WP_003110888.1	type IV pilus assembly protein PilM	Machinery gene
CSC28_RS29825 (CSC28_5916)	-	5965651..5968122 (+)	2472	WP_012077723.1	penicillin-binding protein 1A	-
CSC28_RS29830 (CSC28_5917)	-	5968227..5969495 (-)	1269	WP_003148779.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37983.48 Da
• Isoelectric Point: 4.5405

>NTDB_id=290455 CSC28_RS29820 WP_003110888.1 5964402..5965466(-) (pilM) [Pseudomonas paraeruginosa strain AR441]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=290455 CSC28_RS29820 WP_003110888.1 5964402..5965466(-) (pilM) [Pseudomonas paraeruginosa strain AR441]
GTGCTAGGGCTCATAAAAAAGAAAGCGAATACCTTACTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAACGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAATCGGCCGTGGTG
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACGATCGAGATGGAGGCCGGGCTTTCCGAGGACGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAGGTCGCCATCGACTTCGAGGTCCAGGGTCTTTCCGCGC
GCAATCCCGAGCGCGTGGACGTGCTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTGCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGTTCCTATGCGCTGCTGAGCAGTCAGTTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTGGTGGACATCGGCGCGACCATGACCACGCTCAGCGTCCTGCACAACGGCC
GCACCATCTATACCCGCGAGCAGTTGTTCGGCGGACGCCAGCTCACCGAGGAGATCCAGCGGCGCTACGGGCTTTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGTGGTCTTCCGGATGACTACGACAGCGAAGTCCTGCGTCCGTTCAAGGACGC
CGTGGTCCAGCAGGTTTCCCGTTCGCTGCAGTTCTTCTTCGCCGCCGGCCAGTTCAATGACGTCGACTACATCGTCCTGG
CGGGCGGCACGGCGTCGATCCAGGACCTCGACCGGCTGATTCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAACGCCGGCGCCCTGGCCAGCGACGCGCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.899	100	0.562
comM	Acinetobacter nosocomialis M2	55.899	100	0.562
comM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466