Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BTJ40_RS16835 Genome accession   NZ_CP029064
Coordinates   3829584..3831296 (+) Length   570 a.a.
NCBI ID   WP_108734179.1    Uniprot ID   A0A2S1JVC6
Organism   Microbulbifer sp. A4B17     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3824584..3836296
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BTJ40_RS16820 (BTJ40_16820) - 3825420..3826586 (-) 1167 WP_108734176.1 isoprenylcysteine carboxylmethyltransferase family protein -
  BTJ40_RS16825 (BTJ40_16825) - 3827423..3828598 (-) 1176 WP_108734177.1 serine hydrolase -
  BTJ40_RS16830 (BTJ40_16830) - 3828688..3829167 (-) 480 WP_108734178.1 pilin -
  BTJ40_RS16835 (BTJ40_16835) pilB 3829584..3831296 (+) 1713 WP_108734179.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BTJ40_RS16840 (BTJ40_16840) pilC 3831461..3832675 (+) 1215 WP_108734180.1 type II secretion system F family protein Machinery gene
  BTJ40_RS16845 (BTJ40_16845) pilD 3832681..3833565 (+) 885 WP_108734181.1 A24 family peptidase Machinery gene
  BTJ40_RS16850 (BTJ40_16850) coaE 3833671..3834279 (+) 609 WP_108734182.1 dephospho-CoA kinase -
  BTJ40_RS16855 (BTJ40_16855) yacG 3834332..3834547 (+) 216 WP_108734183.1 DNA gyrase inhibitor YacG -
  BTJ40_RS16860 (BTJ40_16860) mutT 3834874..3835272 (-) 399 WP_108734184.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62257.16 Da        Isoelectric Point: 5.0831

>NTDB_id=289799 BTJ40_RS16835 WP_108734179.1 3829584..3831296(+) (pilB) [Microbulbifer sp. A4B17]
MSTPPLSGLAKKLVADNILDETTALSTLKAAQRARQTFAQHVIETKLVKSKELAVIASQAFGSPLMDLSSFNFEQLPRDV
LDEKLINKHFALPLYQRGNRLFIAVADPTNLAGLDEINFNTGLNTDAVLVEADKLSKAIESYLSNGEDIGAGLEGIDDEE
LDSLDVSNGEDNQDNDDAPGGDEAPVVRFVNKVLLDAIRTGASDIHFEPYEKSYRVRLRTDGVLHEVAKPPIQLAPRISA
RLKVMSKMDISERRVPQDGRIKMKLSKTKAIDFRVNSLPTLWGEKIVLRILDPSSAKLGIDALGYEDEQKKIYMDALAQP
QGMILVTGPTGSGKTVSLYTGLNILNTPERNISTAEDPVEINLEGINQVNVSSKVGLDFAEALRSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLMNMGVPSFNIATSVSVIIAQRLARRLCGECKKPTTLPSEVLKAEGF
EAVTIPEKEWTIFQPVGCEHCSKGYKGRVGVYEVVRITDGIARIIMEGGNSIQIADQAKSEGFNNLRVSALRKVVMGVTS
LEEANRVTKD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=289799 BTJ40_RS16835 WP_108734179.1 3829584..3831296(+) (pilB) [Microbulbifer sp. A4B17]
ATGAGCACCCCCCCCTTAAGCGGCCTTGCCAAAAAATTGGTGGCAGATAATATTCTCGATGAGACAACTGCCCTATCGAC
ACTAAAGGCAGCGCAAAGGGCCAGGCAGACTTTTGCCCAGCATGTTATAGAGACCAAGCTCGTAAAGAGTAAAGAGCTCG
CTGTTATTGCATCACAGGCCTTTGGCTCACCTCTGATGGACCTCAGCAGCTTTAATTTTGAGCAGCTTCCCAGGGATGTT
CTAGATGAAAAATTGATCAATAAACATTTTGCTTTACCTCTCTACCAAAGGGGAAATCGGCTTTTTATTGCTGTTGCGGA
CCCCACTAACCTGGCGGGTCTGGATGAAATCAACTTTAATACCGGCCTGAATACCGATGCCGTTCTGGTGGAGGCCGATA
AACTCAGCAAGGCGATAGAGAGCTATCTTTCCAACGGAGAGGATATTGGAGCGGGTCTTGAAGGTATAGACGATGAGGAG
CTGGACTCTTTAGATGTCAGCAATGGTGAAGATAACCAGGACAATGACGATGCCCCGGGTGGCGATGAGGCGCCGGTGGT
CCGTTTTGTCAATAAAGTACTGCTCGATGCCATCCGTACCGGCGCATCTGATATTCATTTTGAGCCCTACGAAAAATCGT
ACCGCGTACGACTGCGCACCGACGGGGTTCTCCACGAAGTGGCCAAGCCCCCGATTCAGCTAGCACCCCGTATTTCTGCG
CGCCTAAAGGTGATGTCGAAAATGGATATCTCCGAGCGACGCGTGCCGCAGGATGGCCGTATCAAAATGAAGCTGTCTAA
AACCAAGGCCATTGACTTCCGAGTAAACAGCCTCCCAACGTTATGGGGAGAGAAAATTGTACTGCGTATCCTGGATCCAT
CGTCAGCCAAACTGGGGATTGATGCTCTCGGCTATGAAGATGAGCAAAAAAAGATCTACATGGATGCACTGGCTCAACCT
CAAGGAATGATTCTGGTTACAGGCCCTACAGGGTCAGGAAAAACGGTATCCCTCTATACAGGCCTAAATATTCTCAACAC
GCCAGAACGCAATATATCAACCGCCGAGGACCCAGTAGAAATCAACCTGGAAGGCATCAACCAGGTTAACGTGTCTTCCA
AGGTAGGACTGGACTTCGCTGAAGCCCTTCGCTCCTTCTTACGACAGGACCCCGATATCGTCATGGTGGGAGAGATTCGG
GACTTAGAGACAGCCGAGATCGCCATCAAGGCCGCACAAACCGGCCACTTGGTTCTTTCAACTCTCCACACCAATTCTGC
ACCTGAAACTCTCACTCGCCTTATGAATATGGGTGTACCCAGCTTCAATATTGCAACCTCTGTTAGCGTTATTATTGCCC
AGCGCCTAGCGAGACGACTCTGTGGTGAATGTAAAAAACCAACGACTCTTCCAAGTGAAGTATTGAAAGCCGAAGGCTTT
GAAGCTGTGACTATTCCTGAAAAAGAGTGGACAATCTTTCAACCTGTCGGTTGTGAGCACTGCTCCAAAGGGTACAAAGG
GCGTGTTGGTGTGTATGAAGTGGTTCGCATCACCGATGGCATAGCAAGAATTATAATGGAAGGAGGCAATTCAATTCAGA
TCGCTGACCAGGCAAAATCAGAGGGGTTCAATAACCTGAGGGTGTCCGCACTACGCAAGGTTGTTATGGGAGTAACCAGC
CTAGAGGAAGCTAACAGGGTTACCAAAGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-147)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S1JVC6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.595

100

0.607
  pilB Acinetobacter baylyi ADP1

58.916

100

0.591
  pilB Legionella pneumophila strain ERS1305867

54.497

99.474

0.542
  pilB Vibrio campbellii strain DS40M4

51.499

99.474

0.512
  pilB Vibrio parahaemolyticus RIMD 2210633

54.82

92.807

0.509
  pilB Vibrio cholerae strain A1552

53.962

92.982

0.502
  pilF Neisseria gonorrhoeae MS11

46.996

99.298

0.467
  pilB/pilB1 Synechocystis sp. PCC 6803

43.555

89.825

0.391

Multiple sequence alignment