Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   BTJ40_RS16845 Genome accession   NZ_CP029064
Coordinates   3832681..3833565 (+) Length   294 a.a.
NCBI ID   WP_108734181.1    Uniprot ID   A0A2S1JVD6
Organism   Microbulbifer sp. A4B17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3827681..3838565
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BTJ40_RS16830 (BTJ40_16830) - 3828688..3829167 (-) 480 WP_108734178.1 pilin -
  BTJ40_RS16835 (BTJ40_16835) pilB 3829584..3831296 (+) 1713 WP_108734179.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BTJ40_RS16840 (BTJ40_16840) pilC 3831461..3832675 (+) 1215 WP_108734180.1 type II secretion system F family protein Machinery gene
  BTJ40_RS16845 (BTJ40_16845) pilD 3832681..3833565 (+) 885 WP_108734181.1 A24 family peptidase Machinery gene
  BTJ40_RS16850 (BTJ40_16850) coaE 3833671..3834279 (+) 609 WP_108734182.1 dephospho-CoA kinase -
  BTJ40_RS16855 (BTJ40_16855) yacG 3834332..3834547 (+) 216 WP_108734183.1 DNA gyrase inhibitor YacG -
  BTJ40_RS16860 (BTJ40_16860) mutT 3834874..3835272 (-) 399 WP_108734184.1 8-oxo-dGTP diphosphatase MutT -
  BTJ40_RS16865 (BTJ40_16865) argJ 3835282..3836484 (-) 1203 WP_108734185.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 294 a.a.        Molecular weight: 32352.39 Da        Isoelectric Point: 6.6919

>NTDB_id=289801 BTJ40_RS16845 WP_108734181.1 3832681..3833565(+) (pilD) [Microbulbifer sp. A4B17]
MLEVLSTYPALLIGSAFILGLLIGSFLNVVILRLPVMMEREYKRDFYSYFGTEPSAEEQKELEKPFNLILPHSHCPKCQV
EIKAWQNIPIISYLLLRGKCGSCGTPISKRYPMVELVTGILTAIVVWQLGFTWQALAGCFFTWALVALTGIDFDKQLLPD
SITLPLLWAGLLINIWGVFTSLQDAVIGAIAGYLSLWLVFHIFKIVTGKEGMGAGDFKILAAIGAWFGWQMLPLVILLSA
AVGAVAGISWSIAVGRDRNLPIAFGPYLAGAGWIAMLWGDQIMGWYFNISGLTS

Nucleotide


Download         Length: 885 bp        

>NTDB_id=289801 BTJ40_RS16845 WP_108734181.1 3832681..3833565(+) (pilD) [Microbulbifer sp. A4B17]
ATGTTAGAAGTATTATCAACATACCCAGCGCTGCTCATAGGTAGCGCTTTTATTTTAGGCCTCCTTATAGGCAGCTTCCT
CAACGTCGTAATCCTGCGCCTCCCCGTTATGATGGAGCGGGAATATAAGCGGGATTTCTACAGCTACTTTGGCACAGAGC
CATCTGCCGAAGAGCAGAAAGAGTTAGAAAAGCCTTTTAACCTGATACTTCCACACTCCCATTGTCCAAAATGCCAGGTT
GAGATCAAAGCCTGGCAAAATATCCCCATCATTAGCTACCTTTTACTTCGCGGCAAGTGCGGCTCCTGCGGTACCCCGAT
TTCCAAGCGCTACCCAATGGTGGAATTGGTTACCGGTATTTTGACCGCTATTGTTGTCTGGCAGCTGGGTTTCACCTGGC
AGGCACTAGCTGGATGTTTCTTTACCTGGGCTCTAGTCGCCCTGACAGGTATTGATTTTGACAAACAATTACTCCCAGAC
AGCATCACCTTGCCTTTGCTCTGGGCCGGTTTACTGATCAATATTTGGGGAGTATTTACCTCTTTGCAGGATGCAGTGAT
CGGCGCTATCGCCGGTTATCTGTCACTTTGGCTGGTCTTCCATATCTTTAAGATTGTTACCGGTAAGGAAGGCATGGGAG
CCGGGGACTTTAAAATCCTGGCGGCTATTGGAGCCTGGTTTGGCTGGCAAATGCTGCCGCTGGTGATACTGCTTTCCGCT
GCTGTTGGCGCAGTTGCTGGCATTTCTTGGAGTATCGCAGTTGGGCGGGATCGCAACCTGCCCATTGCTTTTGGCCCCTA
TCTCGCTGGTGCCGGCTGGATCGCAATGCTCTGGGGTGATCAAATCATGGGTTGGTATTTCAATATCTCTGGACTTACCA
GCTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(18-128)

Type IV leader peptidase family (Peptidase_A24)

(140-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S1JVD6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

51.601

95.578

0.493
  pilD Acinetobacter baumannii D1279779

51.439

94.558

0.486
  pilD Acinetobacter nosocomialis M2

51.079

94.558

0.483
  pilD Vibrio campbellii strain DS40M4

48.611

97.959

0.476
  pilD Vibrio cholerae strain A1552

47.766

98.98

0.473

Multiple sequence alignment