Detailed information NTG0028879

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: RBAM_RS12075
• Genome accession: NC_009725
• Coordinates: 2510024..2510638 (-)
• Length: 204 a.a.
• NCBI ID: WP_012118026.1
• Uniprot ID: A7Z6X3
• Organism: Bacillus velezensis FZB42
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2505024..2515638

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RBAM_RS12045 (RBAM_023850)	rpsT	2505327..2505593 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
RBAM_RS12050 (RBAM_023860)	holA	2505610..2506651 (-)	1042	Protein_2346	DNA polymerase III subunit delta	-
RBAM_RS12055 (RBAM_38745)	-	2506691..2506842 (-)	152	Protein_2347	hypothetical protein	-
RBAM_RS12060 (RBAM_38750)	-	2506883..2507017 (+)	135	WP_003152870.1	YqzM family protein	-
RBAM_RS12065 (RBAM_023870)	comEC	2507036..2509387 (-)	2352	WP_012118025.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
RBAM_RS12070 (RBAM_023880)	-	2509388..2509957 (-)	570	WP_003152868.1	ComE operon protein 2	-
RBAM_RS12075 (RBAM_023890)	comEA	2510024..2510638 (-)	615	WP_012118026.1	helix-hairpin-helix domain-containing protein	Machinery gene
RBAM_RS12080 (RBAM_023900)	comER	2510697..2511518 (+)	822	WP_012118027.1	late competence protein ComER	-
RBAM_RS12085 (RBAM_023910)	-	2511587..2512324 (-)	738	WP_012118028.1	class I SAM-dependent DNA methyltransferase	-
RBAM_RS12090 (RBAM_023920)	rsfS	2512321..2512677 (-)	357	WP_007408260.1	ribosome silencing factor	-
RBAM_RS12095 (RBAM_023930)	yqeK	2512694..2513254 (-)	561	WP_012118029.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
RBAM_RS12100 (RBAM_023940)	-	2513244..2513813 (-)	570	WP_012118030.1	nicotinate-nucleotide adenylyltransferase	-
RBAM_RS12105 (RBAM_023950)	yhbY	2513824..2514114 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
RBAM_RS12110 (RBAM_023960)	aroE	2514108..2514950 (-)	843	WP_012118031.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22195.40 Da
• Isoelectric Point: 6.4888

>NTDB_id=28879 RBAM_RS12075 WP_012118026.1 2510024..2510638(-) (comEA) [Bacillus velezensis FZB42]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINMASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=28879 RBAM_RS12075 WP_012118026.1 2510024..2510638(-) (comEA) [Bacillus velezensis FZB42]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATATGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

3D structure

• AlphaFold DB: A7Z6X3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA	Latilactobacillus sakei subsp. sakei 23K	32.328	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363