Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EHC66_RS03310 Genome accession   NZ_CP034294
Coordinates   637024..637482 (-) Length   152 a.a.
NCBI ID   WP_029847971.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain 20140829008-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 632024..642482
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EHC66_RS03290 (EHC66_03355) coaE 632545..633159 (-) 615 WP_031847490.1 dephospho-CoA kinase -
  EHC66_RS03295 (EHC66_03360) pilD 633160..634029 (-) 870 WP_015297252.1 A24 family peptidase Machinery gene
  EHC66_RS03300 (EHC66_03365) pilC 634094..635317 (-) 1224 WP_140103791.1 type II secretion system F family protein Machinery gene
  EHC66_RS03305 (EHC66_03370) pilB 635342..637027 (-) 1686 WP_140103792.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EHC66_RS03310 (EHC66_03375) pilA 637024..637482 (-) 459 WP_029847971.1 pilin Machinery gene
  EHC66_RS03315 (EHC66_03385) nadC 637748..638635 (-) 888 WP_140103793.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EHC66_RS03320 (EHC66_03390) ampD 638728..639279 (+) 552 WP_005462574.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EHC66_RS03325 (EHC66_03395) pdhR 639685..640452 (+) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 16193.62 Da        Isoelectric Point: 9.0377

>NTDB_id=288500 EHC66_RS03310 WP_029847971.1 637024..637482(-) (pilA) [Vibrio parahaemolyticus strain 20140829008-1]
MKTNKQKKQQGFTLIELMIVVAVIGVLAAIAMPQYQKYVAKSEVASVLATLTGAKTNVEAYTVENGLFPNNVSDASPTAL
GVPDMPLGSVVFKRDDNTDGGTIEFTFTSVTSGASSLVATKNLTLTRTKEKGSWACSSTNLENELLPKTCKK

Nucleotide


Download         Length: 459 bp        

>NTDB_id=288500 EHC66_RS03310 WP_029847971.1 637024..637482(-) (pilA) [Vibrio parahaemolyticus strain 20140829008-1]
ATGAAAACGAATAAACAGAAGAAGCAGCAAGGTTTTACGCTGATTGAATTAATGATTGTGGTGGCAGTGATTGGTGTCTT
GGCGGCGATTGCAATGCCACAGTACCAAAAATACGTTGCCAAAAGCGAAGTAGCATCTGTTTTAGCCACACTTACTGGTG
CAAAAACTAACGTAGAAGCATACACAGTAGAAAACGGCTTATTTCCAAACAATGTCTCTGACGCATCACCGACGGCTTTG
GGTGTACCAGACATGCCGCTGGGAAGTGTAGTGTTTAAAAGGGATGATAATACTGATGGTGGTACAATAGAATTTACTTT
TACCTCAGTAACTAGCGGAGCAAGCTCCTTAGTAGCGACCAAAAATTTAACACTCACTAGAACAAAAGAAAAAGGGTCTT
GGGCTTGTTCATCTACCAACCTAGAAAATGAACTTCTCCCAAAAACCTGCAAAAAATGA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(40-151)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae strain A1552

46.753

100

0.474
  pilA Vibrio cholerae C6706

46.753

100

0.474
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

46.753

100

0.474
  pilA Pseudomonas aeruginosa PAK

42.763

100

0.428
  pilA Acinetobacter baumannii strain A118

42.657

94.079

0.401
  pilA Vibrio parahaemolyticus RIMD 2210633

46.721

80.263

0.375
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.356

96.053

0.368